Effect of occupational exposure to epichlorohydrin on the frequency of chromosome aberrations in peripheral lymphocytes

Šrám, Radìm J.; Landa, Leoš; Samková, I

doi:10.1016/0165-7992(83)90143-4

Cited by 15 publications

(1 citation statement)

References 6 publications

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“…Measuring the frequency of chromosomal damage in humans exposed to occupational and environmental clastogens has been a priority in public health studies for decades, and an increased level of CAs in population groups is currently interpreted as evidence of genotoxic exposure and early biologic effects on DNA (Albertini et al 2000; Šrám 1981; Šrám et al 1983; Waters et al 1999). However, before using CAs as a marker of cancer risk, it is essential to establish not only the presence of an association with exposure but also the link with cancer occurrence (WHO 2001).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in Lymphocytes of Healthy Subjects and Risk of Cancer

Rössner

Boffetta

Ceppi³

et al. 2005

Environ Health Perspect

165

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There is evidence that increased frequency of chromosomal aberration (CA) in peripheral blood lymphocytes is a predictor of cancer, but further data are needed to better characterize CA as marker of cancer risk. From the archives of 15 laboratories we gathered cytogenetic records of 11,834 subjects who were free of cancer at the moment of blood drawing and who underwent cytogenetic examination for preventive purposes in the Czech Republic during 1975–2000. We linked these records to the national cancer registry, revealing a total of 485 cancer cases. Subjects were classified according to the percentiles of CA distribution within each laboratory as low (0–33rd percentile), medium (34–66th percentile), and high (66–100th percentile). Subjects were further classified by occupational exposure and by subclass of CA. We found a significant association between the overall cancer incidence and the presence of chromosome-type aberrations [relative risk (RR) for high vs. low CA level = 1.24; 95% confidence interval (CI), 1.03–1.50] but not chromatid-type aberrations. Stomach cancer showed a strong association with frequency of total CA (RR = 7.79; 95% CI, 1.01–60.0). The predictivity of CA observed in subjects exposed to various classes of carcinogens did not significantly differ from the group of nonexposed subjects. This study contributes to validation of CA as a predictive marker of cancer risk, in particular, of stomach cancer; the association between CA frequency and cancer risk might be limited to chromosome-type aberrations.

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Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in Lymphocytes of Healthy Subjects and Risk of Cancer

Rössner

Boffetta

Ceppi³

et al. 2005

Environ Health Perspect

165

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Mass spectrometric identification of a candidate biomarker peptide from thein vitro interaction of epichlorohydrin with red blood cells

et al. 2001

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The reaction products of epichlorohydrin with human alpha- and beta- globins, obtained through in vitro incubation of these compounds and red blood cells, were determined by using reversed-phase high-performance liquid chromatography (RP-HPLC), electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization tandem mass spectrometry. The alpha-globin was much more reactive than the beta-globin. At low incubation ratios, approximating the order of magnitude of epichlorohydrin concentration as found in workplaces, the only modified peptide still detectable was the 62-90 belonging to the alpha-chain and carrying an incremental mass of 92 u on either His72 or His89. Given that the two peptides co-eluted in a single chromatographic peak during RP-HPLC separation, they could be chosen as suitable biomarkers for quantification in the setting up of a new methodology for the biological monitoring of persons occupationally exposed, replacing currently known procedures.

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Cytogenetic Analysis and Occupational Health

Šrám

Rössner

Chromosomal Alterations

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Effect of occupational exposure to epichlorohydrin on the frequency of chromosome aberrations in peripheral lymphocytes

Cited by 15 publications

References 6 publications

Chromosomal Aberrations in Lymphocytes of Healthy Subjects and Risk of Cancer

Chromosomal Aberrations in Lymphocytes of Healthy Subjects and Risk of Cancer

Mass spectrometric identification of a candidate biomarker peptide from thein vitro interaction of epichlorohydrin with red blood cells

Cytogenetic Analysis and Occupational Health

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