Effect of Nutritional Supplementation on Oxidative Stress and Hormonal and Lipid Profiles in PCOS-Affected Females

Dubey, Pallavi; Reddy, Sireesha; Boyd, S.Gregory; Bracamontes, Christina; Sanchez, Sheralyn; Chattopadhyay, Munmun; Dwivedi, Alok

doi:10.3390/nu13092938

Cited by 40 publications

(31 citation statements)

References 79 publications

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“…Compared with healthy counterparts, body fat distribution is different in women with PCOS due to the predominant accumulation of visceral fat and abdominal obesity [ 3 , 12 , 32 ]. Accompanying PCOS, abnormal adipose tissue metabolism and associated chronic low-grade inflammation are a significant source of reactive oxygen species [ 14 , 33 ]. Excess free radicals with reduced antioxidant activity—including glutathione, an important small-molecule antioxidant observed in patients with PCOS—may be responsible for exacerbating oxidative stress [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

Chełchowska¹,

Jurczewska

Gajewska³

et al. 2023

Antioxidants

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Metabolic disorders present in women with polycystic ovary syndrome (PCOS) and the associated risk of obesity may result in increased oxidative stress and reproductive failure. Therefore, we evaluated the concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), and reductase (GR), as well as nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associating protein1 (Keap1) in the serum of 56 women with PCOS divided according to the visceral to subcutaneous fat surface ratio (VAT/SAT) and waist-to-hip ratio (WHR) values. Antioxidant parameter levels were measured by competitive inhibition enzyme immunoassay technique. As the VAT/SAT ratio and WHR increased, we observed significantly higher concentrations of GSSG and Keap1 protein and a lower value of the GSSG/GSH ratio (R-index), which is considered an index of cellular redox (p < 0.05). Negative correlations were found between the R-index and body weight, BMI, WHR, subcutaneous and visceral fat surface and the VAT/SAT ratio, and total body fat; positive links were found with fat free mass and total body water. Opposite associations were noted between GSSG level and the aforementioned body composition parameters. Oxidative stress characterized by a depleted reduced-to-oxidized glutathione index is associated with anthropometric and body composition parameters in women with PCOS. In particular, abdominal obesity expressed by the VAT/SAT ratio and/or WHR seems to have a negative impact on glutathione status, which may lead to a disruption of many biological cell processes. The observed negative association of Keap1 with R-index suggests that the elevated oxidative changes dependent on the VAT/SAT ratio may lead to Nrf2 activation to promote antioxidant enzyme expression. Although the GSH/GSSG index as well as the VAT/SAT ratio appear to be good indicators of oxidative status, studies on a larger group of patients should continue to confirm these links among women with PCOS.

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Section: Discussionmentioning

confidence: 99%

“…Finally, the lack of lipid profile and inflammatory markers may be a further limitation of our study. However, it is well known that patients with PCOS often have abnormal lipid levels, and chronic low-grade systemic inflammation is an important factor in this disease [ 6 , 14 , 33 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

Chełchowska¹,

Jurczewska

Gajewska³

et al. 2023

Antioxidants

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“…In PCOS, follicles have a relative abnormal function of granulosa cells and/or degenerating granulosa cells (GCs), indicating abnormal GC proliferation or death (8). (16).…”

Section: Pcos Is An Androgen Disorder and Ovarian Dysfunction Disease Inmentioning

confidence: 99%

NEDD4L facilitates granulosa cell ferroptosis by promoting GPX4 ubiquitination and degradation

Tang

Jiang

Hua

et al. 2023

Endocrine Connections

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Background: Polycystic ovary syndrome (PCOS) is an androgen disorder and ovarian dysfunction disease in women of reproductive age. The cell death of granulosa cells plays an important role in the development of PCOS. However, the mechanism of granulosa cell death is still unclear. Methods: In the current study, NEDD4L was found to be elevated in PCOS GEO databases and mouse models. The cell viability was analyzed by CCK-8 and FDA staining. The expression of ferroptosis markers was assessed by Elisa and immunofluorescence. The direct interaction of GPX4 and NEDD4L was verified by CO-IP assay. Result: Functionally, results from CCK-8 and FDA staining demonstrated that NEDD4L inhibited the cell viability of KGN cells and NEDD4L increased the levels of iron, malonyldialdehyde (MDA), reactive oxygen species (ROS), and decreased glutathione (GSH) levels. Moreover, the cell death of KGN induced by NEDD4L was blocked by ferroptosis inhibitor, suggesting that NEDD4L regulates KGN cell ferroptosis. Mechanistically, NEDD4L directly interacts with GPX4 and promotes GPX4 ubiquitination and degradation. Conclusion: Taken together, our study indicated that NEDD4L facilitates granulosa cell ferroptosis by promoting GPX4 ubiquitination and degradation and contributes to the development of PCOS.

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“…In the same feeding conditions, the body fat of sterile mice increased and IR appeared, which was the first evidence that intestinal flora was related to IR ( 43 ). The study showed ( 44 ) that compared with normal adult women, PCOS patients had intestinal flora disorder, intestinal mucosal barrier damage, increased intestinal wall permeability, and significantly increased endotoxemia related indicators. Insulin sensitivity is increased in patients with metabolic syndrome who are transplanted with healthy human flora ( 45 ).…”

Section: Relationship Between Intestinal Microecological Disorders An...mentioning

confidence: 99%

Effects of intestinal flora on polycystic ovary syndrome

Liu

2023

Front. Endocrinol.

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Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age. Its clinical characteristics are mainly oligo-ovulation or anovulation, hyperandrogenemia (HA) and insulin resistance (IR). PCOS is considered to be one of the main causes of infertility in women of childbearing age, and its pathogenesis is still unclear. Intestinal flora, known as the “second genome” of human beings, is closely related to metabolic diseases, immune diseases and infectious diseases. At the same time, mounting evidence suggests that intestinal flora can regulate insulin synthesis and secretion, affect androgen metabolism and follicular development, and is involved in the occurrence of chronic inflammation and obesity. The imbalance of intestinal flora is caused by the abnormal interaction between intestinal flora and host cells caused by the change of intestinal microbial diversity, which is related to the occurrence and development of PCOS. The adjustment of intestinal flora may be a potential direction for the treatment of PCOS.

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Effect of Nutritional Supplementation on Oxidative Stress and Hormonal and Lipid Profiles in PCOS-Affected Females

Cited by 40 publications

References 79 publications

Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

NEDD4L facilitates granulosa cell ferroptosis by promoting GPX4 ubiquitination and degradation

Effects of intestinal flora on polycystic ovary syndrome

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