Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures

Cui, Xi-Hua; Murthy, Hosakatte Niranjana; Zhang, Ji-De; Song, Hang-Lin; Jiang, Yin-Ji; Qi, Wenwen; Li, Yong Yi; Paek, Kee-Yoeup; Park, Soyoung

doi:10.1007/s11816-019-00592-7

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Similarly, Lulu et al [ 86 ] achieved successful ARs production on leaf explants using ¾ MS strength and 3.0 mg/L IBA. Consistent results were reported by Cui et al [ 87 ], where ¾ MS with 3.0 mg/L IBA led to improved ARs and metabolite production. Giap et al [ 88 ], using cotyledons as explants and testing various PGR combinations, established that MS with 1.5 mg/L NAA and 0.1 mg/L BA is optimal for ARs induction.…”

Section: Techniques For Improving the Synthesis Of Bioactive Compound...supporting

confidence: 90%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

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Section: Techniques For Improving the Synthesis Of Bioactive Compound...supporting

confidence: 90%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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“…All of them are able affect the free radical production and lipid peroxidation 6 . Recent studies also relevant that echinacoside and caffeic acid derivative have weak anti-bacterial and antiviral effects and are protectants against reactive oxygen species 20,39 . The antioxidant activity could be ascribed to the polyphenolic components 13 , such as flavonoids 34 , phenolic acids 35 or phenolic diterpenes 9 .…”

Section: Discussionmentioning

confidence: 99%

Improving growth properties and phytochemical compounds of Echinacea purpurea (L.) medicinal plant using novel nitrogen slow release fertilizer under greenhouse conditions

Ahmadi

Samadi

Rahimi

2020

Sci Rep

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Medicinal plant production is most important than other agricultural plants due to their phytochemical compounds effects on human health. Paying attention to plant nutrition requirement is so important. In order to assess the effect of nitrate (NO 3 −) dosage supplies from two types of fertilizers on growth and phytochemical properties of Echinacea purpurea rhizomata cum radicibus, an experiment with completely simple design was carried out under greenhouse conditions. two types of fertilizers (new invented nitrogen (N) slow release fertilizer and urea chemical fertilizer) at three dosages (50, 100, and 150 mM) were applied. Plant growth parameters and total phenolic (TPC), total flavonoids (TFC), polysaccarides content, essential oil content, caffeic acid derivatives, and anti-radical scavenging activities of E. purpurea were assessed. The results showed the significant (p ≤ 0.01) differences among treatments, both in growth and phytochemical properties. Using of n slow release, especially in 150 mM dosage, significantly increased all the plant growth and phytochemical properties. The dried E. purpurea rhizomata cum radicibus contained more caftaric acid (max 12.56 mg g −1 DW) and chicoric acid (max 7.56 mg g −1 DW) than other derivatives. Despite the impact of heavy metals on yield and growth of E. purpurea, the concentration of all heavy metals and micronutrients (boron (B), cadmium (cd), copper (cu), iron (fe), manganese (Mn), molybdenum (Mo), nickel (ni), lead (pb), and zinc (Zn)) in studied soil and fertilizer samples was less than United States environmental protection Agency (USepA) limits of contamination. Based on the results, using of n slow release fertilizers can improve phytochemical properties of the plant due to its polymeric structure and can be a suitable substitution of chemical fertilizers, especially in medicinal plants growth. Many of researchers are pay attention to herbal and medicinal plants as natural remedies due to their bioactive compounds recently 1,2. Echinacea purpurea (L.) Moench is one of the most important medical plants of Asteraceae family with various pharmacological effects 3. The major three species of Echinacea are known as Echinacea angustifolia, Echinacea purpurea, and Echinacea pallida. E. purpurea is native to much of the United States and its morphological properties are also known as purple coneflower with orange and cone-shaped flowering head that change to purple, rose, or white petals during June to September 4. It has a narrow leaves and three feet height stem with dark, thick and pungent rhizomata cum radicibus 5. In the environment, E. purpurea grows randomically along water canals with low densities for affective harvest for commercial purposes 6. Comparing of other Echinacea species, E. purpurea is the most able species to adjust to new conditions. It is resistance to heat or cold weather conditions, easy to grow, and flaunt high efficiency 7,8. Numerous scientific

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“…Our findings confirmed the results of Ataslar et al (2019) and Kołodziej et al (2019) that gypsophila saponins can act as natural antioxidants to protect cells from oxidative damage. Effect of culture media on root biomass, saponin and flavonoid production Root biomass production It is crucial to find the optimum type and concentration of the nutrient medium for AR cultures which is considered a critical factor for root growth and phytochemicals accumulation (Cui et al, 2020). The data of root growth parameters (FW, DW, % dry matter, RGR, and growth index) are compiled in Table 2.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

“…Successful attempts to produce phytochemical compounds on a large scale have been developed in industrial bioreactors for hairy root (HR) and adventitious root (AR) cultures (Jiang et al, 2015;Wu et al, 2018;Pandey et al, 2022a), which will allow industrial production of natural bioactive compounds from plant roots. Studies show that root growth and their capability for the biosynthesis of various biologically active compounds are affected by types and levels of carbohydrate source, plant growth regulators (PGRs), culture media, elicitation as well as culture condition (Jiao et al, 2014;Wu et al, 2018;Cui et al, 2020;Jiao et al, 2020).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Establishment of Gypsophila paniculata root culture for biomass, saponin, and flavonoid production

Elateeq

GABR

ABDELKAWY

et al. 2022

Not Bot Horti Agrobo

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Baby’s breath (Gypsophila paniculata L.) roots are valuable as pharmaceuticals due to the content of triterpenoid saponins and other bioactive phytochemicals. However, the long root harvest period and fluctuation of these constituent’s content are among the constraints to traditional agricultural production. The present study shows an efficient and reliable liquid root culture of G. paniculata cv. ‘Perfecta’ established in a shake flask system using different auxins, media, and sugars. Hairy root (HR) induction in G. paniculata through Rhizobium rhizogenes-mediated transformation was also investigated. α-Naphthaleneacetic acid (NAA) was optimized for adventitious roots (AR) biomass, saponin, and flavonoid production at 1 or 2 mg/L compared to IBA. Full strength Gamborg’s medium (B5) recorded higher saponin content; however, the highest yield of total saponin and total flavonoids was achieved by full strength Murashige and Skoog's (MS) medium. Sucrose was more essential for root growth and accumulated total saponins and flavonoids rather than fructose and glucose. Polymerase chain reaction (PCR) analysis showed that G. paniculata HR carried rolC gene of R. rhizogenes A4 strain in its genome but not virD2 gene. Compared to non-transformed root, saponin content of leaf and stem-derived HR was 2.7 and 2.3-fold, while total flavonoid content was 2.1 and 2.0-fold, respectively. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was also higher in HR extracts than in non-transgenic roots. This study established an efficient protocol for G. paniculata root cultures for sustainable production of important natural saponins and flavonoids.

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Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures

Cited by 9 publications

References 15 publications

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Improving growth properties and phytochemical compounds of Echinacea purpurea (L.) medicinal plant using novel nitrogen slow release fertilizer under greenhouse conditions

Establishment of Gypsophila paniculata root culture for biomass, saponin, and flavonoid production

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