Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Lentinula (Lentinus) edodes

Abstract

Lentinula (Lentinus) edodes, strain LS4, produces manganese‐dependent peroxidase (MnP) and laccase, but not lignin peroxidase, when grown on a defined medium with glucose as sole carbon source. MnP production is suppressed by nitrogen whereas highest levels of laccase were observed when the fungus was grown under high nitrogen (26 mM) conditions. Both the titre and time of appearance of MnP were affected by the concentration of Mn in the culture medium with highest enzyme levels recorded in cultures supplemented with 1.1 ppm Mn. Purified MnP from L. edodes LS4 has an apparent Mr of 59000 and a pI of 5.6, and differs in several respects from a MnP isolated from L. edodes grown on a commercial wood substrate.

Impact of fine litter chemistry on lignocellulolytic enzyme efficiency during decomposition of maize leaf and root in soil

“…The xylanase and cellulase activities of all soil suspension samples were determined by reference to a standard curve produced with purified endoxylanase from Thermobacillus xylanilyticus (Debeire-Gosselin et al 1992) and commercial cellulase (322 U ml -1 , endo-b-glucanase purified from Aspergillus niger, Megazyme) at concentrations ranging between 0.01 and 0.1 U ml -1 for the cellulase and between 0.001 and 0.01 mU ml -1 for xylanase, both in sodium acetate buffer (50 mM, pH 5). Laccase activity was assayed as described by Buswell et al (1995), Floch et al (2007. One hundred milliliters of 0.55 mM laccase substrate (ABTS, 2,2 0 -azinobis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt, Sigma) was prepared by using sodium acetate buffer (50 mM, pH 4.5) followed by vigorous shaking with a magnetic stirrer.…”

Laccase production by Lepista sordida

“…Some species, like the well-studied Phanerochaete chrysosporium, present laccase activity only when nitrogen concentration falls to limiting levels (10,12,15). On the other hand, L. edodes exhibits decreasing laccase activity following the depletion of nitrogen (3,15). The culture media used in the present study was efficient to stimulate laccase activity in L. sordida with the nitrogen sources used ammonium phosphate (0.15 g/L), yeast extract (0.1 g/L) and ammonium molibdate (0.011 g/L).…”

Biodegradation of Synthetic Dyes: A Mycoremediation Approach for Degradation/Decolourization of Textile Dyes and Effluents

“…Kim et al [66] demonstrated the presence of H 2 O 2 dependent enzyme activity to declourize the dye Brilliant Blue R by the fungus Pleurotus ostreatus. Buswell et al [67] reported that the production of the extracellular ligninolytic enzymes is strongly affected by the nature and amount of the nutrients, especially nitrogen (N) and microelements, in the growth substrate. The basidiomycetous fungus Ganodermaa lucidum has been found positive for the removal of Rhodamine-B and Sandolan rhodine dyes.…”