Effect of normal and irradiated marrow grafts on the haemopoietic recovery after a sublethal irradiation

Delrez, Marc; Haot, J.; Betz, E.H.

doi:10.1007/bf02137308

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…The phenomenon can be effectively prevented by partial pro tection of the bone marrow during irradiation. Similar inhibition can be obtained by intravenous injection of normal bone marrow cells up to 6 days following sublethal irradiation of the animals [11,12].…”

Section: Morphology and Conditions Of Developmentmentioning

confidence: 48%

Origin, Morphologic and Functional Characteristics of a New Lymphoid Cell Type in Irradiated Mouse Bone Marrow

Haot¹,

Betz²,

Simar³

et al. 1974

Acta Haematol

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After sublethal irradiation, an immature lymphoid cell type with characteristic ultrastructure was found in the bone marrow and thymus of adult mice of different strains. Normally this cell type has only been detected in the embryo or new-born. Grafting normal, adult bone marrow suppresses, while thymectomy enhances the proliferation of these cells in the irradiated organism. Immunologic and functional tests suggest that the cells have either an embryonic origin, or are derivatives of haemopoietic stem cells and function either as precursors for the immunocompetent B and T cells, or in an entirely different lymphoid system.

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Section: Morphology and Conditions Of Developmentmentioning

confidence: 48%

Origin, Morphologic and Functional Characteristics of a New Lymphoid Cell Type in Irradiated Mouse Bone Marrow

Haot¹,

Betz²,

Simar³

et al. 1974

Acta Haematol

Self Cite

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“…In both instances even with shielding, all animals died. We next assessed if the standard radiation dose to generate bone marrow chimeras, 16 a much lower dose than radiation to the head and neck, resulted in corneal changes. Thus, we used a similar protocol that allows sublethal irradiation, which affects the corneas without resulting in death of animals.…”

Section: Methodsmentioning

confidence: 99%

A Novel Murine Model of Radiation Keratopathy

Harris

Yamaguchi

Hamrah

2018

Invest. Ophthalmol. Vis. Sci.

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PurposeRadiation therapy results in severe chronic keratopathy and dry eye disease. We developed a novel mouse model for radiation keratopathy to allow future mechanistic studies.MethodsSix to 8-week-old BALB/c mice underwent sublethal irradiation to the head only from a Cesium-137 irradiator, 2 × 550 rad, 3-hours apart. Irradiated mice were clinically evaluated by corneal fluorescein staining (CFS) at 1, 2, and 3 months, after which corneas were excised and immunofluorescence histochemistry performed with anti-CD45, anti-MHC class II, and anti-β-tubulin antibodies.ResultsThe survival rate after irradiation was 100%. Mice demonstrated significant CFS and hair loss around the eyes. Corneal nerve density decreased in the central and peripheral corneas (P < 0.01) at 2 and 3 months, respectively. CD45+ immune cell densities increased in the central and peripheral corneas (P < 0.005, P < 0.001) at 2 and 3 months, respectively. MHC class II, a sign of antigen presenting cell activation, significantly increased after irradiation in the central and peripheral corneas at 2 and 3 months (P = 0.02). A strong inverse correlation was noted between decreased corneal nerves and increase in CD45+ cells in the central cornea at 2 (P = 0.04, r = −0.89) and 3 months (P = 0.03, r = −0.91) after irradiation.ConclusionsWe present a model of radiation keratopathy and demonstrate significant nerve loss and increase in immune cell influx and activation within months. This model will enable future investigations to understand the effects of radiation therapy on the eye, and to study mechanisms of neuro-immune crosstalk in the cornea.

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Influence of a mixture of chemical protectors on the lymphoid regeneration of bone marrow and thymus in irradiated mice

et al. 1978

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Effect of normal and irradiated marrow grafts on the haemopoietic recovery after a sublethal irradiation

Cited by 6 publications

References 5 publications

Origin, Morphologic and Functional Characteristics of a New Lymphoid Cell Type in Irradiated Mouse Bone Marrow

Origin, Morphologic and Functional Characteristics of a New Lymphoid Cell Type in Irradiated Mouse Bone Marrow

A Novel Murine Model of Radiation Keratopathy

Influence of a mixture of chemical protectors on the lymphoid regeneration of bone marrow and thymus in irradiated mice

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