Effect of non‐surgical periodontal treatment on clinical parameters and the numbers of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans at adult periodontitis sites

Abstract

This study demonstrates the usefulness of Q-PCR for enumerating putative pathogens in clinical periodontal specimens and that the numbers of the three organisms in all sites decrease with non-surgical periodontal therapy.

Comparison of nonsurgical periodontal therapy with oral hygiene instruction alone for chronic periodontitis

“…It eliminates or reduces the microbial counts of subgingival flora after improving the clinical characteristics and oral health of patients (10). The present findings show that, as compared with OHI alone, NSPT significantly improved all clinical variables (GBI, PPD, and PAL) except VPI at 3 months after treatment.…”

“…These findings corroborate those of previous findings, which also found no association between clinical variables and periodontal pathogens such as A. actinomycetemcomitans and P. intermedia (10). However, the present findings contradict those of studies reporting a positive correlation of number of P. gingivalis with PPD and PAL (39,40).…”

“…In the current study, the baseline prevalences of tested pathogens were higher than in previous studies (10,28), perhaps because of differences in ethnicity (29) and the microbial diagnostic techniques used (30). At 3 months after treatment, the prevalences of pathogens generally declined, but the changes were not significant.…”

“…The presence and concentration of specific periodontal pathogens are important in disease initiation and progression, and numerous studies have thus investigated these pathogen characteristics. Studies comparing pre-and post-treatment prevalence, percentage, and frequency of detection of pathogenic species at healthy and non-healthy sites have yielded varying results (9,10).…”

Detecção por PCR de Porphyromonas gingivalis e dos genótipos fimA II, IV e ragB+ no biofilme subgengival, antes e 180 dias após o tratamento periodontal convencional e associado à terapia antimicrobiana em pacientes fumantes com periodontite crônica

“…As cepas ragB positivas foram encontradas em cerca de 20% dos isolados de P. gingivalis, sendo frequentemente detectadas em bolsas periodontais mais profundas do que em bolsas rasas, indicando uma associação do genótipo ragB com virulência (ANZAI, 2004; A terapêutica para a periodontite crônica usualmente é mecânica, por meio de raspagem e alisamento radiculares e meticulosa orientação de higiene bucal, cujo efeito imediato é o rompimento e desorganização do biofilme dentário subgengival, resultando em melhora das características clínicas, particularmente do nível de inserção clínico e profundidade de bolsas (BROCHUT et al, 2005;CUGINI et al, 2000;DOUNGUDOMDACHA et al, 2001;MOMBELLI et al, 2000;PAWLOWSKI et al, 2005;SOCRASNKY, 2006;XIMÉNEZ-FYVIE;XIMÉNEZ-FYVIE et al, 2000), bem como nos parâmetros microbiológicos (COLOMBO et al, 2005;CUGINI et al, 2000;HAFFAJEE et al, 1997;XIMÉNEZ-FYVIE;XIMÉNEZ-FYVIE et al, 2000). GAMONAL, 1998;LÓPEZ et al, 2006;SLOTS, 2004;TING, 2002;WINKEL et al, 2001 (BURT, 2005;LAMONT;JENKINSON, 1998;SANZ et al, 2004;SLOTS, 1996 P. gingivalis vêm sendo fortemente associada à patogênese da periodontite.…”