Effect of Non-Genetic Factors on Different Genetic Group of Buck’s Semen Quality and Quantity at National Livestock Breeding Center, Nepal

Abstract

This study was conducted to assess the quality and quantity aspects of different genetic group of buck’s semen used for Artificial Insemination (AI) at National Livestock Breeding Centre (NLBC), Pokhara. For this, records from different breeds and genetic groups of buck semen collected over 3 years (2014-2016) were analyzed. Parameters such as volume, density, mass motility, initial motility concentration and post thaw motility were analysed for various non-genetic factors like year, season, green based feeding. Data were analyzed by least square method using Harvey 1990 software package. The result showed that different genetic group of bucks (P<0.001) have highly significant effect (P=0.00) on volume of semen. Significant interaction (P=0.01, 0.001) was observed between year and season on volume and density of the semen. Similarly, different genetic group of bucks had significant effect on density (P=0.03). Year showed a significant effect (P=0.02) on initial motility of the buck semen. Similarly, significant interaction between year and season on initial motility percentage (P=0.01) and post thaw motility percentage (P=0.00) was found. Likewise, year has a significant effect (P=0.01) on the Post thaw motility percentage (P=0.03) of the semen. Also, a significant effect of different breed of buck was found on post thaw motility percentage (P=0.00). In addition, the average post thaw motility percentage semen of all the genetic groups of bucks was above 52% which is considered as a good percentage for the success of AI. This result might be due to use of egg yolk free extender which is soya-based protein. Moreover, adoption of new buck semen processing technologies might further improve the semen quality and long term storability along with better management and distribution of these buck semen by Government of Nepal through livestock unit at different municipalities will improve the conception rate and save huge effort and investment for the importation of buck semen.

Current Status and Future Prospects of Artificial Insemination in Goats in Nepal: A Review

“…Then the cuvette is inserted in a sample container of a photometer which is pre-set with the buck semen (Bastola et al, 2018). As, an explicit issue in the protection of goat semen has been found to have the detrimental impact of seminal plasma on the viability of the spermatozoa in diluents containing egg yolk or in milk-based media (Leuboeuf et al, 2000).…”

“…As, an explicit issue in the protection of goat semen has been found to have the detrimental impact of seminal plasma on the viability of the spermatozoa in diluents containing egg yolk or in milk-based media (Leuboeuf et al, 2000). Andromed R , a soybean lecithin-based extender, has been proposed as a substitution of egg yolk as animal source diluents (Bastola et al, 2018).…”

“…The success of AI was discovered to depend largely on the quality of cryopreserved semen, an imperative tool for the successful execution of a breeding program to set up the pure line breed of goat, which in turn determined the conception rate (Bastola et al, 2018). The quality of semen is a crucial factor that determined the overall process of AI.…”

“…To aid the buck's semen collection, a doe indicating proof of estrus i.e., teaser doe is placed near so the contributor buck gets stimulated and can mount her. A group researchers suggested that all the components of AV used for semen collection including the outer rubber cylinder, inner rubber line, rubber band, cone, and collecting tube should be sterilized before collection of semen using an autoclave machine (Bastola et al, 2018). At the National livestock breeding center of Nepal, the tube with collected semen is first labeled with the identification of the breed, eventually, the mouth is sealed with aluminum foil and then kept in a water bath at 37 degrees celsius for five minutes (Bastola et al, 2018).…”

“…A group researchers suggested that all the components of AV used for semen collection including the outer rubber cylinder, inner rubber line, rubber band, cone, and collecting tube should be sterilized before collection of semen using an autoclave machine (Bastola et al, 2018). At the National livestock breeding center of Nepal, the tube with collected semen is first labeled with the identification of the breed, eventually, the mouth is sealed with aluminum foil and then kept in a water bath at 37 degrees celsius for five minutes (Bastola et al, 2018). Besides these, large amount of semen was also found to have been exported in Nepal from other countries to meet the demand for goat (Paudel et al, 2007).…”