Effect of non-adhering dressings on promotion of fibroblast proliferation and wound healing in vitro

Abstract

Non-adhering dressings are commonly used during granulation, tissue formation, and re-epithelialization. Elucidating cytotoxic effects and influence on proliferation/migration capacity of cells like fibroblasts is of interest. Dressings’ effects were investigated by comprehensive in vitro approach: (1) MTT assay measuring cell viability after direct contact, (2) ATP assay determining effects on cell proliferation, and (3) scratch wound assay featuring an in vitro wound healing model. One cotton-based dressing with vaseline (vas) was included in the study and four polyester dressings containing vas and technology-lipido-colloid matrix (TLC), carboxymethylcellulose (CMC), hydrocolloid (HC), or glycerin (gly) as additives. A polyamide dressing with vas + CMC and three silicone-based dressings (AT, CC, M) were tested. Polyester + vas + CMC did not negatively affect cell viability or proliferation but it was found that fibroblast layers appeared more irregular with decreased F-actin network structure and tubulin density possibly leading to hampered scratch closure. Silicone AT, polyester + gly and polyamide + vas + CMC caused distinct cell damage. The latter two further reduced cell viability, proliferation and scratch healing. From the overall results, it can be concluded that cotton + vas, polyester + TLC, polyester + vas + HC and the silicone dressings CC and M have the potential to prevent damage of newly formed tissue during dressing changes and positively influence wound healing.

Update on the role of antiseptics in the management of chronic wounds with critical colonisation and/or biofilm

“…A pre‐requisite of any wound dressing is to shield the wound, offering not only a physical protective barrier but also a first line of defence against antimicrobial invasion of the wound. Intimate contact between the wound dressing and those cells that are key to the wound healing process is inevitable, so it is vital that a wound dressing demonstrates good cell compatibility 109 . Cytotoxic effects of a wound dressing would reduce the viability, proliferation, and migration of cells involved in the wound healing process, and so decrease the healing rate 109 .…”

Advances in Healthcare Electronics Enabled by Triboelectric Nanogenerators

“…Fibroblast cell proliferation is an essentially biological process for human wound healing. [59,60] Cell proliferation is the original biological growth, development, reproduction and fibroblast cells help synthesize extracellular matrix and collagen for a wound. [61,62] In 2019, Hu et al fabricated a rotating disc TENG (RD-TENG) to achieve alternating current output to stimulate fibroblast cells (L929) through interdigital electrodes, as shown in Figure 3.…”

“…Fibroblast cell proliferation is an essentially biological process for human wound healing. [ 59,60 ] Cell proliferation is the original biological growth, development, reproduction and fibroblast cells help synthesize extracellular matrix and collagen for a wound. [ 61,62 ] In 2019, Hu et al.…”

Ski promotes proliferation and inhibits apoptosis in fibroblasts under high‐glucose conditions via the FoxO1 pathway

“…Among these factors, the change in fibroblast function is an important reason for the damage of epithelialisation and delayed healing of diabetic wounds, 3,4 and this function is related to the decreased proliferation and increased apoptosis of fibroblasts in diabetic wounds 3,4 . Therefore, targeted therapy to improve the function of fibroblasts is an important method to promote the healing of refractory diabetic wounds 4‐7 . The regulation of fibroblast proliferation and apoptosis is primarily achieved by improving the environment, such as increasing growth factors 4,5,7 .…”

“…3,4 Therefore, targeted therapy to improve the function of fibroblasts is an important method to promote the healing of refractory diabetic wounds. [4][5][6][7] The regulation of fibroblast proliferation and apoptosis is primarily achieved by improving the environment, such as increasing growth factors. 4,5,7 The direct targeting of fibroblasts to improve their proliferation and anti-apoptotic effects may become a new and more effective way to treat refractory diabetic wounds.…”