Effect of nitrogen sources and fermentation conditions on bacillus sp. R2 chitinase production

Cheba, Ben Amar; Zaghloul, Taha I.; El-Mahdy, Ahmed R.; EL-Massry, Mohamad Hisham

doi:10.1016/j.promfg.2018.03.043

Cited by 15 publications

(14 citation statements)

References 25 publications

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“…Chitinase production is induced by the presence of (1,4)-glycosidic bonds in the substrate. Though various substrates, such as powdery chitin, colloidal chitin, chitosan, crab shell, shrimp powder, demineralized crab, wheat bran, sugarcane bagasse, and cellobiose, are used for the production of chitinase [ 64 , 79 , 84 , 85 ], colloidal chitin is the substrate of choice at the commercial scale, due to its higher productivity [ 64 , 86 ]. Colloidal chitin, chitin flakes, and other chitinous substrates, such as dimeric N-acetylglucosamine, are good inducers of chitinase.…”

Section: Microbial Fermentation For Chitinase Productionmentioning

confidence: 99%

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Poria

Rana

Kumari

et al. 2021

Biology

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Chitinases are a large and diversified category of enzymes that break down chitin, the world’s second most prevalent polymer after cellulose. GH18 is the most studied family of chitinases, even though chitinolytic enzymes come from a variety of glycosyl hydrolase (GH) families. Most of the distinct GH families, as well as the unique structural and catalytic features of various chitinolytic enzymes, have been thoroughly explored to demonstrate their use in the development of tailor-made chitinases by protein engineering. Although chitin-degrading enzymes may be found in plants and other organisms, such as arthropods, mollusks, protozoans, and nematodes, microbial chitinases are a promising and sustainable option for industrial production. Despite this, the inducible nature, low titer, high production expenses, and susceptibility to severe environments are barriers to upscaling microbial chitinase production. The goal of this study is to address all of the elements that influence microbial fermentation for chitinase production, as well as the purifying procedures for attaining high-quality yield and purity.

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Section: Microbial Fermentation For Chitinase Productionmentioning

confidence: 99%

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Poria

Rana

Kumari

et al. 2021

Biology

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“…Further increase in inoculum size had a detrimental effect due to limited nutrient availability for cell biomass and faster growth of the culture. 40 Also, multiplication of cells to sufficient number that will utilize the substrate for maximum production does not require lower inoculum size. Lower inoculum size may not rapidly initiate growth and increase metabolite production such as bacteriocin.…”

Section: Resultsmentioning

confidence: 99%

Growth Enhancement of Lactic Acid Bacteria for Production of Bacteriocin Using a Local Condiment Supplemented with Nitrogen Sources

2020

TJNPR

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Bacteriocin produced by lactic acid bacteria (LAB) inhibits the growth of pathogenic and spoilage microorganisms in foods. This metabolite can be produced using locally sourced condiment such as Ogi, a fermented cereal gruel. The study investigated the production of bacteriocin from nitrogen supplemented Ogi medium by the isolated Lactobacillus spp. LAB was isolated from Ogi, a locally fermented grain. The test isolate was selected after LAB isolates were screened for antibacterial activity against selected indicator microorganisms; Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The formulated media investigated were Ogi (O), Ogi and peptone (OP), Ogi and yeast extract (OYE), and Ogi, peptone and yeast extract (OPY). The effect of substrate (Ogi) concentration and inoculum size on the bacteriocin production was also determined. Among the identified LAB, Lactobacillus plantarum had the highest antibacterial activity with a zone of inhibition of 22.33 ± 1.20 mm against Escherichia coli. For all the formulated growth media used and inoculated with L. plantarum, OPY medium had the highest bacteriocin concentration of 15.14 ± 0.44 mg/L whereas the least, 5.76 ± 0.96 mg/L, was from Ogi medium. Effect of substrate (Ogi) concentration and inoculum size on bacteriocin production revealed that 0.6% w/v and 0.9 x 10 6 cfu/mL recorded the highest concentration of 14.29 ± 0.05 and 16.28 ± 0.50 mg/L, respectively. Utilization of Ogi as a basal substrate for bacteriocin production may create an avenue for further research on the large scale production of this metabolite.

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“…When investigating the effect of fermentation conditions and nitrogen sources on the production of chitinase by Bacillus sp. R2, Cheba et al (2018) found that incubation at 30°C resulted in the highest observed chitinase activity. Cultivation of B. cereus 8A at 30°C was also found to more appropriate for the production of the bacteriocin cerein 8A as opposed to cultivation at 25 or 37°C with the latter resulting in decreased bacteriocin activity (Bizani and Brandelli 2004).…”

Section: Temperature Salinity Ph and Oxygen Availabilitymentioning

confidence: 95%

“…This means that chitinase production can be significantly influenced by cultivation conditions and media constituents (Cheba et al . ).…”

Section: Secondary Metabolites and Hydrolytic Enzymes Produced By Bacmentioning

confidence: 97%

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Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides

Horak

Engelbrecht

Rensburg

et al. 2019

J of Applied Microbiology

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Root‐knot nematodes are destructive phytopathogens that damage agricultural crops globally, and there is growing interest in the use of biocontrol based on rhizobacteria such as Bacillus to combat Meloidogyne species. It is hypothesized that nematicidal activity of Bacillus can be attributed to the production of secondary metabolites and hydrolytic enzymes. Yet, few studies have characterized these metabolites and their identities remain unknown. Others are speculative or fail to elaborate on how secondary metabolites were detected or distinguished from primary metabolites. Metabolites can be classified based on their origin as either intracellular or extracellular and based on their function, as either primary or secondary. Although this classification is in general use, the boundaries are not always well defined. An understanding of the secondary metabolite and hydrolytic enzyme classification of Bacillus species will facilitate investigations aimed at bionematicide development. This review summarizes the significance of Bacillus hydrolytic enzymes and secondary metabolites in bionematicide research and provides an overview of known classifications. The importance of appropriate cultivation conditions for optimum metabolite and enzyme production is also discussed. Finally, the use of metabolomics for the detection and identification of nematicidal compounds is considered.

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Effect of nitrogen sources and fermentation conditions on bacillus sp. R2 chitinase production

Cited by 15 publications

References 25 publications

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Growth Enhancement of Lactic Acid Bacteria for Production of Bacteriocin Using a Local Condiment Supplemented with Nitrogen Sources

Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides

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