Effect of nitrogen sources and 2, 4-D treatment on indirect regeneration of ginger (Zingiber officinale Rosc.) using leaf base explants

Abstract

Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium (NH 4+) and nitrate (NO 3-) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number (7.33± 0.33) and length (88.33±4.40) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

“…In addition, a developed root system is the crucial step in plant vegetative propagation, which ensures a high survival rate of micropropagated plantlets during the acclimatization stage [ 25 ]. In vitro root induction of ginger is enhanced by auxins supplementation in the culture medium [ 26 , 27 ]. Different types and concentrations of auxin varyingly affected in vitro root induction in different Zingiberaceae species [ 27 , 28 ].…”

“…In vitro root induction of ginger is enhanced by auxins supplementation in the culture medium [ 26 , 27 ]. Different types and concentrations of auxin varyingly affected in vitro root induction in different Zingiberaceae species [ 27 , 28 ]. Determining the optimum type and concentration of auxin can significantly enhance the in vitro root induction of ginger and, subsequently, facilitates acclimatization and successful establishment of the in vitro-raised plantlets in the field conditions.…”

Optimizing Photoautotrophic Micropropagation Conditions for Ginger

“…In-vitro root induction in ginger is further enhanced by supplementing the culture medium with auxins (Abbas et al 2011;Mehaboob et al 2019). Different types and concentrations of auxins have varying effects on in-vitro root induction in different Zingiberaceae species (Mehaboob et al 2019;Jualang et al 2015). Determining the optimum type and concentration of auxin can signi cantly enhance the in-vitro root induction of ginger and facilitate the acclimatization and successful establishment of the in-vitro plantlets in eld conditions.…”

“…Mohamed et al (2011) found that shootlets became highly rooted when half strength B5 medium was supplemented with 1.0 mg/L NAA. In-vitro root induction in ginger is further enhanced by supplementing the culture medium with auxins (Abbas et al 2011;Mehaboob et al 2019). Different types and concentrations of auxins have varying effects on in-vitro root induction in different Zingiberaceae species (Mehaboob et al 2019;Jualang et al 2015).…”

Optimizing Photoautotrophic Micropropagation Conditions for Ginger

“…found that shootlets became highly rooted when half strength B5 medium was supplemented with 1.0 mg/L NAA. In-vitro root induction in ginger is further enhanced by supplementing the culture medium with different nutrients(Abbas et al 2011;Mehaboob et al 2019). Determining the optimum type and concentration of nutrition can signi cantly enhance the in-vitro root induction of ginger and facilitate the acclimatization and successful establishment of the in-vitro plantlets in eld conditions(Mehaboob et al 2019;Jualang et al 2015).…”