Effect of Nitrogen on Fucoxanthin Accumulation in the Diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin

Ryabushko, V. I.; Zheleznova, S. N.; Nekhoroshev, M. V.

doi:10.1615/interjalgae.v19.i1.70

Cited by 5 publications

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“…According to [ 44 ], the FX concentration in the cyanobacteria and microalgae biomass was calculated. At a wavelength of 488 nm, the extinction coefficient was calculated to be 1280 mL/g·cm [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Study of the Antimicrobial Potential of the Arthrospira platensis, Planktothrix agardhii, Leptolyngbya cf. ectocarpi, Roholtiella mixta nov., Tetraselmis viridis, and Nanofrustulum shiloi against Gram-Positive, Gram-Negative Bacteria, and Mycobacteria

Lykov,

Salmin,

Gevorgiz

et al. 2023

Marine Drugs

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The incidence of diseases brought on by resistant strains of micro-organisms, including tuberculosis, is rising globally as a result of the rapid rise in pathogenic micro-organism resistance to antimicrobial treatments. Secondary metabolites with potential for antibacterial activity are produced by cyanobacteria and microalgae. In this study, gram-positive (S. aureus, E. faecalis) and gram-negative (K. pneumoniae, A. baumannii, P. aeruginosa) bacteria were isolated from pulmonary tuberculosis patients receiving long-term antituberculosis therapy. The antimicrobial potential of extracts from the cyanobacteria Leptolyngbya cf. ectocarpi, Planktothrix agardhii, Arthrospira platensis, Rohotiella mixta sp. nov., Nanofrustulum shiloi, and Tetraselmis (Platymonas) viridis Rouchijajnen was evaluated. On mouse splenocytes and peritoneal macrophages, extracts of cyanobacteria and microalgae had inhibitory effects. In vitro studies have shown that cyanobacteria and microalgae extracts suppress the growth of bacteria and mycobacteria. At the same time, it has been demonstrated that cyanobacterial and microalgal extracts can encourage bacterial growth in a test tube. Additionally, the enhanced fucoxanthin fraction significantly reduced the development of bacteria in vitro. In a mouse experiment to simulate tuberculosis, the mycobacterial load in internal organs was considerably decreased by fucoxanthin. According to the information gathered, cyanobacteria and microalgae are potential sources of antibacterial compounds that can be used in the manufacturing of pharmaceutical raw materials.

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Section: Methodsmentioning

confidence: 99%

Study of the Antimicrobial Potential of the Arthrospira platensis, Planktothrix agardhii, Leptolyngbya cf. ectocarpi, Roholtiella mixta nov., Tetraselmis viridis, and Nanofrustulum shiloi against Gram-Positive, Gram-Negative Bacteria, and Mycobacteria

Lykov,

Salmin,

Gevorgiz

et al. 2023

Marine Drugs

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“…Marine semi-benthic tychoplanktonic pennate non-colonial diatom Cylindrotheca closterium (Ehrenberg) Reimann & J. C. Lewin, 1964 (Bacillariaceae, Bacillariophycidae) (formerly, synonym Nitzschia closterium), cosmopolitan in temperate and tropical waters [11], initially isolated from the Black Sea coastal waters, was kept in the Museum of Alive Phytoplankton Cultures of the Department of Physiology of Algae in A. O. Kovalevsky Institute of Marine Biological Research RAS (former Institute of Biology of the Southern Seas). This species was cultured intensively using new modified artificial medium for mariculture purposes [23], and sub-samples of it from the batch culture were kindly provided for our experiments by Ms Zheleznova S. N.…”

Section: Methodsmentioning

confidence: 99%

“…No toxic, or any other negative effect of ubiquitous marine diatom Cylindrotheca closterium (Ehrenberg) Reimann & J. C. Lewin, 1964 (Bacillariaceae, Bacillariophycidae) (formerly Nitzschia closterium) was observed on reproduction of copepods Calanus helgolandicus and Acartia clausi [4]. Unlike some other diatom species known to produce toxins, this diatom was never associated with any harmful toxins release, and to date, is widely cultured for far going pharmacological purposes as a transporter of Ag associated nannoparticles [20], or as a source of fucoxanthin [23]. Some coastal harpacticoid copepods even prefer C. closterium as a food source to other diatoms [29].…”

mentioning

confidence: 99%

How diatom Cylindrotheca closterium vanquish invasive copepod Oithona davisae

Khanaychenko

2018

mbj

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Some diatoms are rich food for herbivorous copepods, while others are toxic for their recruitment. No negative effect of diatom Cylindrotheca closterium was ever observed for copepods, and some estuarine copepods preferred it as a food. Data on grazing diatoms by abundant now in the Black Sea coastal waters invasive copepod Oithona davisae are still contradictory. Interaction of O. davisae and C. closterium, both having high colonizing potential and both typical for coastal waters, was studied in experimental culture. Two weeks after inoculation of C. closterium the cultured O. davisae was drastically fouled by globulous conglomerates of diatom cells. Diatom cells in "colonies" on copepod exoskeleton were interconnected by means of adhesive substances at one of their flexible ends at the point-wise areas at various parts of copepods exoskeleton, and the opposite flexible ends performed various circular roll-over fan-shaped movements around the axis passing through the point of their attachment. "Colonies" behaved as integrated aggressive organisms against any approaching flagellate and prevented normal locomotion of copepods. Herein we present the first report on epizoic behavior of C. closterium: quick disastrous colonization of alive copepods O. davisae by diatom "colonies" led to total extinction of cyclopoid experimental population while alive diatoms formed dense network on copepods degenerative tissues.

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“…In the composition of the photosynthetic apparatus of C. closterium, the content of fucoxanthin under certain conditions of cultivation can reach 1.5-1.7 % of the dry weight of the algae [36]. An increase of the nitrogen concentration in the nutrient medium F leads to a noticeable increase in the productivity of the cells and the accumulation of fucoxanthin in the culture.…”

Section: A B C Dmentioning

confidence: 99%

Different aspects of studying a diatom Cylindrotheca closterium (Ehrenberg) Reimann et Lewin 1964 in natural and laboratory conditions

Ryabushko¹,

Balycheva²,

Bondarenko³

et al. 2019

mbj

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The article summarizes original and literary data on different aspects of studying Cylindrotheca closterium (Ehrenberg) Reimann et Lewin 1964 in two biotopes – phytoplankton and microphytobenthos – of the Black Sea, the Sea of Azov, and the Sea of Japan for the period from 1976 to 2016. The aim of the work is to present the results of the study mainly of own data on the morphology, systematics and ecology of C. closterium in different seas and under cultivation in the laboratory. Information on the history of the species origin and its nomenclature changes is given. C. closterium belongs to the phylum Bacillariophyta, class Bacillariophyceae, order Bacillariales Hendey 1937, family Bacillariaceae Ehrenb. 1831, genus Cylindrotheca Rabenhorst 1859 emend. Reim. et Lewin 1964. This benthoplanktonic species occurs in the plankton, in littoral and sublittoral zones of the seas. The species is marine and brackish-water; it is a cosmopolite common in different geographical zones of the World Ocean. The results of studying alga by various methods under natural and experimental conditions in light and transmission electron microscopes of C. Zeiss LIBRA-120 are presented. The quantitative data of C. closterium were determined by direct counting of the cells in the Goryaev’ camera (V = 0.9 mm³) in light microscopes BIOLAM L-212, C. Zeiss Axioskop 40 with the program AxioVision Rel. 4.6 at 10×40, 10×100, and Olympus BX41 (Tokyo, Japan) with lenses UPLanF140× and 100×1/30 oil immersion. Cultivation of C. closterium was carried out in the cumulative mode on the nutrient medium F, volume of 1 L under light intensity of 13.7 klx and temperature of +20…+21 °C. Morphology data of this species from different seas were obtained. The average cell sizes of C. closterium are: 25–260 µm length, 1.5–8 µm width; 12–25 fibulae in 10 µm. The results of cultivation in the laboratory conditions showed that the average cell sizes reached 148.17 µm (length) and 8 µm (width) at the temperature of +19…+20 °C and light intensity of 13 klx; length of cells reached 162.12 µm in the exponential phase of growth and 172.07 µm – in the stationary phase. C. closterium has an important practical significance as a source of fucoxanthin, since this alga is intensively cultivated for production of biologically active substances. Our experimental data showed that during laboratory cultivation the fucoxanthin concentration in a diatom biomass can reach 11 mg·g-1 of dry mass. The new data obtained are relevant and important; they can be used in different fields of science and medicine. The seasonal dynamics of population abundance of C. closterium in different ecotopes (epizoon of invertebrates and their food spectra, epiphyton of bottom vegetation, periphyton of the experimental and anthropogenic substrates of the different seas) is presented for the first time. The maximum abundance of the species population (65.6·10³ cells·cm-2) was registered in the epizoon of the mussel Mytilus galloprovincialis Lam. in March at the water temperature of +7.7 °C at a depth of 2.5 m in the Black Sea. The maximum abundance was registered in the epiphyton of green algae (896·10³ cells·cm-2) and in the periphyton of asbestos plates (728·10³ cells·cm-2) in August at the water temperature of +24.5 °C in the Sea of Japan. The abundance dynamics of C. closterium natural populations in the local habitats changed depending on the season, the depth, and the type of substrate. The similarities and differences in the distribution of C. closterium in the sea microphytobenthos are discussed.

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Effect of Nitrogen on Fucoxanthin Accumulation in the Diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin

Cited by 5 publications

References 0 publications

Study of the Antimicrobial Potential of the Arthrospira platensis, Planktothrix agardhii, Leptolyngbya cf. ectocarpi, Roholtiella mixta nov., Tetraselmis viridis, and Nanofrustulum shiloi against Gram-Positive, Gram-Negative Bacteria, and Mycobacteria

Study of the Antimicrobial Potential of the Arthrospira platensis, Planktothrix agardhii, Leptolyngbya cf. ectocarpi, Roholtiella mixta nov., Tetraselmis viridis, and Nanofrustulum shiloi against Gram-Positive, Gram-Negative Bacteria, and Mycobacteria

How diatom Cylindrotheca closterium vanquish invasive copepod Oithona davisae

Different aspects of studying a diatom Cylindrotheca closterium (Ehrenberg) Reimann et Lewin 1964 in natural and laboratory conditions

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