Effect of nitrogen and mineral composition of the high-concentrated wort made from starch-containing raw materials on the cultivation of yeast

Shiyan, Peter; Mudrak, Tatiana; Kyrylenko, Roman; Kovalchuk, Svetlana N.

doi:10.15587/1729-4061.2017.117357

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Nutrient supplementation is crucial to achieving high ethanol content in worts containing a mixture of sugarcane juice and corn hydrolysates [53]. The yeast's tolerance to ethanol may also depend on the availability of nutrients in each of the musts, as yeast cells have an increased demand for microelements when under stress, as mineral ions play an essential role in maintaining pH, osmotic stability, nutrient transport, and serving as cofactors in fermentation reactions [54].…”

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

“…In our experiment 2, we supplied the worts with 600 mg L −1 of urea, following the recommendation of Kovalchuk et al (2017) [55]. The addition of nitrogen sources can boost the population of physiologically active yeast cells, increasing the chances of success in fermentations with worts containing high sugar concentrations [54]. Moreover, yeast's capacity for biosynthesis and biomass formation is closely linked to the availability of nitrogen in the medium [56].…”

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

“…Moreover, yeast's capacity for biosynthesis and biomass formation is closely linked to the availability of nitrogen in the medium [56]. In conditions of ethanol stress, the assimilation of nitrogenous sources is significantly impacted, as the accumulation of ethanol strongly inhibits the amino acid transport pathway [54].…”

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

“…In addition to the aforementioned effects, ethanol also denatures and inhibits glycolytic enzymes and can lead to the formation of mutant cells [19]. Such a condition reduces the regenerative and fermentative activity of yeasts [54].…”

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

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Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates

Douradinho,

Sica,

Tonoli

et al. 2023

Stresses

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High-gravity fermentation, used for ethanol production from sugarcane, corn, and mixed substrates, offers several benefits. Yeast, a rapidly multiplying unicellular microorganism, can be adapted for high sugar and ethanol tolerance on a lab scale. However, different substrates can enhance fermentation efficiency. Our study consisted of two experiments. In the first, we compared simple batch feeding with a fed-batch system for yeast selection in high-gravity fermentation. We ran eight cycles with increasing initial sugar contents (50 to 300 g L−1). No significant differences were observed in the first seven cycles, but in the eighth, the fed-batch system showed lower glycerol and fructose contents and higher cell viability than the simple batch system. In the second experiment, we used the fed-batch system with 300 g L−1 from sugarcane, corn, and mixed wort. The results showed that mixed wort produced higher ethanol contents and greater fermentation efficiency compared to corn and sugarcane as substrates. In conclusion, our findings indicate that the fed-batch system is more suitable for high-gravity fermentation on a lab scale, and the combination of sugarcane juice and corn can enhance fermentation efficiency, paving the way for integrating these substrates in industrial ethanol production.

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Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

Section: Effects Of Substrate On Fermentation Efficiency and Future P...mentioning

confidence: 99%

See 2 more Smart Citations

Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates

Douradinho,

Sica,

Tonoli

et al. 2023

Stresses

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“…Yeast tolerance to ethanol is intricately connected to the availability of nutrients in the wort. Yeast cells exhibit increased nutrient requirements under stressful conditions, and microelements, including mineral ions, are essential for maintaining pH and osmotic stability, facilitating nutrient transport, and serving as cofactors in fermentation reactions [69]. Mixed wort can increase by about 5% in fermentation efficiency compared to wort with just corn, mainly due to the nitrogen and phosphorus provided by the sugarcane juice, allowing the fermentation to reach higher ethanol contents and lower residual sugars [6,7].…”

Section: Effects Of Wort Composition On Very High Gravity Fermentationmentioning

confidence: 99%

Assessing Ionizing Radiation and Chlorine Dioxide (ClO2) as Potential Aseptization Treatments for Yeast Recycling on Mixed Wort of Corn and Sugarcane in Brazil

Douradinho,

Sica,

Oliveira

et al. 2024

Stresses

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Yeast recycling, which is a common practice in sugarcane ethanol plants, could be expanded if it could be successfully implemented in corn-based ethanol production. However, the process of recycling the yeast remaining after fermentation is hampered by contaminating microorganisms that reduce the fermentation efficiency and compete with the yeast for the fermentable sugars. Currently, antibiotics are used to control microbial contamination. This study proposes chlorine dioxide and electron beam irradiation as alternative control methods for completely inactivating contaminants and minimizing their effect on recycled yeast. For that, wort sterilization using electron radiation (20 kGy) and treatment with a chemical biocide, namely chlorine dioxide (125 mg kg−1), were compared with non-treated wort. Five fermentation cycles were performed using fed-batch systems with 300 g L−1 of fermentable sugars. The results obtained in this study showed the inactivation of contaminants under the effect of electron beam irradiation, which led to an increase in the productivity, yield, and efficiency of fermentation by 0.21 g L−1h−1, 2.6%, and 4.7%, respectively. However, ClO2 did not show promising results in reducing contamination or improving fermentative parameters. Thus, electron beam irradiation of contaminated wort may be a suitable alternative to chemical biocides and would allow the use of recycled yeast in corn-based ethanol plants.

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Selection of the Complex of Enzyme Preparations for the Hydrolysis of Grain Constituents During the Fermentation of the Wort of High Concentration

Mudrak¹,

Kuts²,

Kovalchuk³

et al. 2018

ХНТ

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In this paper, an optimal complex is selected of enzyme preparations for hydrolysis of the components of grain raw materials during fermentation of high concentration wort. When selecting enzyme systems, their effect on the technical and chemical parameters of the fermented wash during the fermentation of wort is investigated. For the research, maize grain with a starch content of 69.0 % was used. Fermentation was carried out with 18–30% of dry matters (DM) in the wort, using the osmophilic yeast strain Saccharomyces cerevisiae DO-16.The recommended concentration of the enzyme preparation Amylex 4 T (the source of the α-amylase enzyme) – 0.4–0.6 units of α-amylase ability/g of starch – is optimal for the concentration 18–27% of DS in the wort. For 30 % of DS, it is practical to use 0.6 units of α-amylase ability/g of starch. With the use of the enzyme preparation Diazyme TGA (the source of the enzyme glucoamylase), the value is 7.5 units of glucoamylase ability/g of starch, alcohol accumulation in fermented washes was 10.51, 13.35, 15.78% vol., according to the wort concentrations 18, 27, 30 %, respectively. It has been established that with the application of the cytolytic enzyme Laminex 750, the concentrations of dissolved carbohydrates and non-dissolved starch have a tendency to decrease. In the samples where the proteolytic enzyme preparation Alphalase AFP was added at a concentration of 0.05 units of proteolytic ability/g of raw materials, there was an increase in the accumulation of yeast cells by 6.5% compared with the reference sample. The recommended concentration of Deltazyme VR XL (the source of β-glucanase and xylanase) is 0.05 units β-glucose/g of raw materials. The addition of a cytolytic and proteolytic enzyme preparation in combination with β-glucanase and xylanase contributed to an increase in the accumulation of ethanol in the washes by 1.7 % compared with the reference sample, and to an almost 33 % decrease in the concentration of dissolved carbohydrates and non-dissolved starch. On the basis of experimental studies, it has been found that using a complex of enzyme preparations – amylolytic (Amylex 4T), saccharifying (Diazyme TGA), proteolytic (Alphalase AFP), cytolytic (Laminex 750), and complex AF β-glucanase and xylanase (Deltazyme VR XL), in various combinations of their concentrations, – contributed to the intensification of the fermentation process of the wort and increased accumulation of the target product, ethanol, by 0.8–1.4 %, depending on the wort concentration. The highest amount of ethanol accumulated at the maximum dosage of additional enzyme preparations.

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Effect of nitrogen and mineral composition of the high-concentrated wort made from starch-containing raw materials on the cultivation of yeast

Cited by 7 publications

References 6 publications

Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates

Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates

Assessing Ionizing Radiation and Chlorine Dioxide (ClO2) as Potential Aseptization Treatments for Yeast Recycling on Mixed Wort of Corn and Sugarcane in Brazil

Selection of the Complex of Enzyme Preparations for the Hydrolysis of Grain Constituents During the Fermentation of the Wort of High Concentration

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