Effect of nitrofurans and NO generators on biofilm formation by Pseudomonas aeruginosa PAO1 and Burkholderia cenocepacia 370

Zaitseva, Julia N.; Граник, В. Г.; Belik, A. Yu.; Кокшарова, О. А.; Khmel, I. A.

doi:10.1016/j.resmic.2009.04.007

Cited by 37 publications

(26 citation statements)

References 16 publications

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“…A previous report determined that low concentrations (500 nM) of the NO donor SNP inhibit biofilm formation when administered at the start or after 24 h of biofilm formation (22). However, studies of P. aeruginosa indicate that SNP can also enhance biofilm formation when administered at higher doses (25 to 100 M) (3,71). This observation prompted us to investigate the effect of NO concentration on gonococcal biofilm formation.…”

Section: Resultsmentioning

confidence: 99%

“…This has been also observed in Pseudomonas aeruginosa (3) and staphylococcal (58) biofilms. However, higher concentrations of NO (M to mM) can also enhance biofilm formation in P. aeruginosa (3,71).…”

mentioning

confidence: 99%

“…We hypothesized that N. gonorrhoeae uses a combination of anaerobic/microaerobic and aerobic metabolism to support biofilm growth. The observations that biofilm formation is more severely attenuated in a norB mutant and that NO can affect P. aeruginosa biofilm in a concentration-dependent manner (3,71) prompted us to investigate the influence of higher concentrations of NO on gonococcal biofilm formation. We found that anaerobic metabolism occurs primarily at the biofilm-surface interface or substrata of gonococcal biofilms and that higher NO concentrations can enhance biofilm formation, especially in the absence of nitrite.…”

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confidence: 99%

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Anaerobic Metabolism Occurs in the Substratum of Gonococcal Biofilms and May Be Sustained in Part by Nitric Oxide

et al. 2010

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Neisseria gonorrhoeae is the etiologic agent of gonorrhea, which has been among the most frequently reported communicable diseases in the United States since 1960. Women frequently do not exhibit symptoms, which can lead to chronic infection. N. gonorrhoeae readily forms biofilms over abiotic surfaces, over primary and transformed cervical epithelial cells, and over cervical tissues in vivo. Biofilms are often associated with chronic infection, which suggests a link between biofilm formation and asymptomatic gonorrhea in women. Proteins involved in anaerobic metabolism and oxidative-stress tolerance are critical for normal biofilm formation of N. gonorrhoeae. Therefore, we examined the spatial profiles of anaerobic respiration in N. gonorrhoeae, using an aniA-gfp transcriptional fusion. Nitric oxide (NO) can elicit biofilm dispersal when present at sublethal concentrations in the surrounding medium. Some reports indicate that NO may also encourage biofilm formation at higher, potentially lethal concentrations. NO is produced by polymorphonuclear lymphocytes (PMNs) and cervical endothelial and epithelial cells. Thus, we also examined the effect of NO on N. gonorrhoeae biofilms. We found that anaerobic respiration occurs predominantly in the substratum of gonococcal biofilms and that expression of aniA is induced over time in biofilms. Treatment with high concentrations of a rapid-release NO donor prevents biofilm formation when supplied early in biofilm development but can also enhance biofilm formation once anaerobic respiration is initiated. NO treatment partially restores biofilm formation in an aniA::kan insertion mutant, which suggests that N. gonorrhoeae in biofilms may use NO as a substrate for anaerobic growth but prefer nitrite.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Anaerobic Metabolism Occurs in the Substratum of Gonococcal Biofilms and May Be Sustained in Part by Nitric Oxide

et al. 2010

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“…It has also been shown that NO stimulates biofilm formation in Nitrosomonas europaea (21), Azospirillum brasilense (22), and Shewanella oneidensis (23) or that NO had no effect on biofilms (24,25). Thus, different bacteria may show individual responses to NO, and hence it is uncertain what the overall effect of NO might be on a natural, mixed-species biofilm community.…”

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confidence: 99%

Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Barnes

Low

Bandi

et al. 2015

Appl Environ Microbiol

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f Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single-and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis (RO) membranes. The potential of PROLI NONOate to control RO membrane biofouling was also examined. Confocal microscopy revealed that PROLI NONOate exposure induced biofilm dispersal in all but two of the bacteria tested and successfully dispersed mixed-species biofilms. The addition of 40 M PROLI NONOate at 24-h intervals to a laboratory-scale RO system led to a 92% reduction in the rate of biofouling (pressure rise over a given period) by a bacterial community cultured from an industrial RO membrane. Confocal microscopy and extracellular polymeric substances (EPS) extraction revealed that PROLI NONOate treatment led to a 48% reduction in polysaccharides, a 66% reduction in proteins, and a 29% reduction in microbial cells compared to the untreated control. A reduction in biofilm surface coverage (59% compared to 98%, treated compared to control) and average thickness (20 m compared to 26 m, treated compared to control) was also observed. The addition of PROLI NONOate led to a 22% increase in the time required for the RO module to reach its maximum transmembrane pressure (TMP), further indicating that NO treatment delayed fouling. Pyrosequencing analysis revealed that the NO treatment did not significantly alter the microbial community composition of the membrane biofilm. These results present strong evidence for the application of PROLI NONOate for prevention of RO biofouling.

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“…Epidural and subarachnoid block were used successfully in patients for non-carcinoid surgery [27,28]. But care must be taken for cardiovascular stability as these procedures can cause hypotension which can be resistant to the usual therapy as sympathomimetics can exacerbate the condition.…”

Section: Regional Anesthesiamentioning

confidence: 99%

Perioperative Management of Carcinoid Syndorme-An Anaesthesiologist’s Perspective

Mvs¹

2016

J Anesth Clin Res

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Occurrence of carcinoid syndrome is very rare. So the expertise and literature on this syndrome is limited. Physiologically active substances that are secreted by the carcinoid tumour cause major problems for the anesthesiologist in the perioperative period. Preoperative preparation of the patient is the most important step that decides the recovery of the patient. Discovery of octreotide lead to the improvement in treating and decreasing the morbidity and mortality during surgery. If the surgery is palliative post-operative management also makes it difficult for the anesthesiologist. Extreme vigilance and common sense had to be applied during the surgery by the anesthesiologist. Pathophysiology, symptomatology, diagnosis and perioperative management are described in this article.

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Effect of nitrofurans and NO generators on biofilm formation by Pseudomonas aeruginosa PAO1 and Burkholderia cenocepacia 370

Cited by 37 publications

References 16 publications

Anaerobic Metabolism Occurs in the Substratum of Gonococcal Biofilms and May Be Sustained in Part by Nitric Oxide

Anaerobic Metabolism Occurs in the Substratum of Gonococcal Biofilms and May Be Sustained in Part by Nitric Oxide

Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Perioperative Management of Carcinoid Syndorme-An Anaesthesiologist’s Perspective

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