Effect of niloticin, a protolimonoid isolated from Limonia acidissima L. (Rutaceae) on the immature stages of dengue vector Aedes aegypti L. (Diptera: Culicidae)

Reegan, Appadurai Daniel; Gandhi, Munusamy Rajiv; Paulraj, Michael Gabriel; Balakrishna, K.; Ignacimuthu, S.

doi:10.1016/j.actatropica.2014.07.002

Cited by 46 publications

(27 citation statements)

References 41 publications

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“…To our knowledge, only the protolimonoid nilocitin has hitherto been reported to exhibit significant growth disruption and morphological deformities against Ae. aegypti larvae and pupae ( Reegan et al 2014) , and the present investigation is therefore the first to isolate melianodiol by conduction a bioassay-guided study of plant constituents active against Ae. aegypti larvae.…”

Section: Discussionmentioning

confidence: 95%

Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti

Sarmento

Miguita

Almeida

et al. 2016

Mem. Inst. Oswaldo Cruz

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A total of 36 ethanol extracts from different anatomical parts of 27 plant species (18 families), native to the Pantanal and Cerrado biomes in Midwest Brazil, was assessed for their effect against Aedes aegypti larvae, the vector of dengue, hemorrhagic dengue, Zika and chikungunya fevers. Only the extract obtained from seeds of Guarea kunthiana (Meliaceae) proved active (LC50 = 169.93 μg/mL). A bioassay-guided investigation of this extract led to the isolation and identification of melianodiol, a protolimonoid, as the active constituent (LC50 = 14.44 mg/mL). Meliantriol, which was also obtained from the bioactive fraction, was nevertheless devoid of any larval toxicity, even at the highest concentration tested (LC50 > 100.0 mg/mL). These results indicate that the larvicidal activity of melianodiol stems from the presence of the carbonyl moiety at C-3 in the 21,23-epoxy-21,24,25-trihydroxy-tirucall-7-ene-type skeleton. The structures of both protolimonoids were established on the basis of spectral methods (1H and 13C NMR and MS). This is the first report on the toxicity of melianodiol against Ae. aegypti larvae. Based on the results, melianodiol can be regarded as a potential candidate for use as an ecologically sound biocontrol agent for reducing the larval population of this vector.

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Section: Discussionmentioning

confidence: 95%

Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti

Sarmento

Miguita

Almeida

et al. 2016

Mem. Inst. Oswaldo Cruz

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“…Notably, confertifolin (i.e., 6,6, 9a-trimethyl-4,5,5a,6,7,8,9,9a-octahydronaphtho[1,2-c]furan-3(1H)-one), isolated from the leaf essential oil of Polygonum hydropiper, led to 100 % egg mortality when tested at 10 ppm on eggs of A. stephensi and C. quinquefasciatus (Maheswaran and Ignacimuthu 2013). Lastly, it is important to note that any of the surveyed researches shed light on the mechanism(s) of action of plantborne ovicides against mosquito vectors, even if Reegan et al (2014) noted that the eggs exposed to sub-lethal doses of niloticin led to growth disruption and morphological deformities in A. aegypti. Further research on this issue is urgently required.…”

Section: Botanical-based Mosquito Ovicidesmentioning

confidence: 98%

“…The WSMoL lectin from Moringa oleifera aqueous seed extract tested against A. aegypti eggs showed an EC 50 of 0.10 mg/ml, while the aqueous seed extract and its 0-60 protein fraction achieved EC 50 values of 0.32 and 0.16 mg/ml, respectively (de Santos et al 2012). The protolimonoid niloticin from the hexane leaf extract of Limonia acidissima was an excellent ovicidal against A. aegypti, since it showed 83.2 % ovicidal activity at 2 ppm concentration 120 h post-treatment (Reegan et al 2014), while 500 ppm of the hexane leaf extract of Limonia acidissima led to egg mortality of 60 and 79.2 % against A. aegypti and C. quinquefasciatus, respectively (Reegan et al 2015). Piperitenone oxide from Mentha spicata var.…”

Section: Botanical-based Mosquito Ovicidesmentioning

confidence: 98%

Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review

Benelli

2015

Parasitol Res

283

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Mosquitoes (Diptera: Culicidae) are a huge threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. Culicidae control is of crucial importance. Mosquito eggs, larvae, and pupae are usually targeted using organophosphates, insect growth regulators, and microbial agents. Indoor residual spraying and insecticide-treated bed nets are also employed. However, these chemicals have negative effects on human health and the environment, and induce resistance in a number of species. Eco-friendly tools have been recently implemented against mosquito vectors, including botanical insecticides. The majority of researches focused on larvicides (745 SCOPUS results, July 2015) and adult repellents (434 SCOPUS results), while limited efforts were conducted to identify effective ovicides of botanical origin (59 SCOPUS results). Here, I review current knowledge on the effectiveness of plant-borne ovicides against major mosquito vectors of medical and veterinary importance. The majority of researches focused on the toxicity of crude extracts, their fractions, or essential oils against three important mosquito vectors, Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. As a general trend, C. quinquefasciatus eggs were the most resistant to botanical ovicides. Five studies proposed selected compounds from plant extracts and essential oils as ovicides effective at few parts per million. However, no efforts were conducted to shed light on possible mechanisms underlying the toxicity of plant-borne ovicides. In the final section, a number of hot issues needing further research and cooperation among parasitologists, entomologists, and researchers working in natural product chemistry are outlined.

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“…Many sporadic dengue cases have been reported from various parts of India (Akram & Ahmed 2005) and a major outbreak was recorded during 2012. During this outbreak, a total of 5376 dengue cases and 39 deaths were detected in the state Tamil Nadu, southern India (Kannan 2012, Reegan et al 2014. The climatological condition also favoured rapid increase of A. aegypti populations.…”

Section: Introductionmentioning

confidence: 99%

Larvicidal and Pupicidal Activities of Alizarin Isolated from Roots of Rubia cordifolia Against Culex quinquefasciatus Say and Aedes aegypti (L.) (Diptera: Culicidae)

et al. 2016

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The mosquitocidal activities of different fractions and a compound alizarin from the methanol extract of Rubia cordifolia roots were evaluated on larvae and pupae of Culex quinquefasciatus Say and Aedes aegypti (L.) (Diptera: Culicidae). Larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10 ppm for fractions and 0.5, 1.0, 1.5 and 2.0 ppm for compound. After 24 h, the mortality was assessed and the LC 50 and LC 90 values were estimated for larvae and pupae. Among the 23 fractions screened, fraction 2 from the methanol extract of R. cordifolia showed good mosquitocidal activity against C. quinquefasciatus and A. aegypti. LC 50 and LC 90 values of fraction 2 were 3.53 and 7.26 ppm for C. quinquefasciatus and 3.86 and 8.28 ppm for A. aegypti larvae, and 3.76 and 7.50 ppm for C. quinquefasciatus and 3.92 and 8.05 ppm for A. aegypti pupae, respectively. Further, the isolated compound alizarin presented good larvicidal and pupicidal activities. LC 50 and LC 90 values of alizarin for larvae were 0.81 and 3.86 ppm against C. quinquefasciatus and 1.31 and 6.04 ppm for A. aegypti larvae, respectively. Similarly, the LC 50 and LC 90 values of alizarin for pupae were 1.97 and 4.79 ppm for C. quinquefasciatus and 2.05 and 5.59 ppm for A. aegypti pupae, respectively. The structure of the isolated compound was identified on the basis of spectroscopic analysis and compared with reported spectral data. The results indicated that alizarin could be used as a potential larvicide and pupicide.

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Effect of niloticin, a protolimonoid isolated from Limonia acidissima L. (Rutaceae) on the immature stages of dengue vector Aedes aegypti L. (Diptera: Culicidae)

Cited by 46 publications

References 41 publications

Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti

Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti

Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review

Larvicidal and Pupicidal Activities of Alizarin Isolated from Roots of Rubia cordifolia Against Culex quinquefasciatus Say and Aedes aegypti (L.) (Diptera: Culicidae)

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