Effect of nifedipine, a calcium channel blocker, on the generation of nitric oxide and interleukin-1β by murine macrophages activated by lipopolysaccharide from Prevotella intermedia

Choe, So-Hui; Choi, Eun‐Young; Hyeon, Jin-Yi; Keum, Bo Ram; Choi, Inhee; Kim, Sung-Jo

doi:10.1007/s00210-020-01958-3

Cited by 8 publications

(9 citation statements)

References 66 publications

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“…In contrast, M2 macrophages are effective in producing anti‐inflammatory responses, with increased production of IL‐10, CCL17, and CCL22 and increased phagocytic activity [24, 36]. This finding reinforces the affirmation that 1,4‐DHP derivatives exert their anti‐inflammatory effects by changing the macrophage phenotype, Choe et al demonstrated that murine macrophages stimulated with LPS and pretreated with nifedipine led to a significant increase in mRNA expression of Arg‐1, Ym‐1, FIZZ1, and TGF‐β, characteristic markers of polarization of M2‐type macrophages [14]. Our results show, in addition to the decrease in the levels of cytokines characteristic of M1 responses, an increase in the phagocytic activity of the macrophages, as well as an increase in the production of the anti‐inflammatory cytokine IL‐10, which is characteristic of the M2 phenotype [37, 38].…”

Section: Discussionsupporting

confidence: 67%

“…In contrast, M2 macrophages are effective in producing anti-inflammatory responses, with increased production of IL-10, CCL17, and CCL22 and increased phagocytic activity [24,36]. This finding reinforces the affirmation that 1,4-DHP derivatives exert their antiinflammatory effects by changing the macrophage phenotype, Choe et al demonstrated that murine macrophages stimulated with LPS and pretreated with nifedipine led to a significant increase in mRNA expression of Arg-1, Ym-1, FIZZ1, and TGF-β, characteristic markers of polarization of M2-type macrophages [14].…”

Section: Groupsupporting

confidence: 68%

“…In our study, the studied derivative of 1,4‐DHP was able to significantly inhibit NO production in vitro. In a recent study, Nifedipine, an important representative of this class of compounds, also demonstrated similar activity, inhibiting NO production in RAW 264.7 cells and peritoneal macrophages derived from BALB/c mice stimulated by LPS [14]. Similarly, Chen and Kitts demonstrated that dihydropyridine derivatives can inhibit intracellular NO production in RAW 264.7 cell culture [15].…”

Section: Discussionmentioning

confidence: 97%

“…1,4-Dihydropyridine analogs (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) were synthesized, as previously reported in the literature by Pollo [16] (Figure 1), following the Hantzsch dihydropyridine synthesis methodology, in a one-pot manner without added catalyst. All the referred substances were purified by column chromatography and characterized by 1 H, 13 C NMR, and high-resolution mass spectrometry (HRMS).…”

Section: Compound Synthesismentioning

confidence: 99%

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Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

Facchin,

Lubschinski,

Moon

et al. 2023

Fundamemntal Clinical Pharma

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IntroductionInflammation is a physiological event that protects the organism against different factors that lead to loss of tissue homeostasis. Dihydropyridine (DHP) derivatives are heterocyclic compounds known for their different biological activities, including anti‐inflammatory activities.ObjectiveTo evaluate the anti‐inflammatory activity of 1,4‐dihydropyridine (1,4‐DHP) derivatives using anti‐inflammatory models in vitro, in RAW264.7 cells induced by lipopolysaccharide (LPS) and in vivo using the acute lung injury (ALI) model in mice.ResultsFifteen compounds derived from 1,4‐DHP were tested in RAW264.7 cells for their cytotoxic effect and cell viability. Thereafter, only the six compounds that showed the highest cell viability were tested for the production or inhibition of the pro‐inflammatory cytokine interleukin 6 (IL‐6). The best compound (compound 4) was tested for its anti‐inflammatory effects in vitro and in vivo, showing inhibition of nitric oxide (NO), pro‐inflammatory cytokines, increased phagocytic activity, and an increase in IL‐10 in vitro. In in vivo tests, compound 4 also reduces the levels of NO, myeloperoxidase (MPO) activity, leukocyte migration, and exudation, as well as reducing the levels of tumor necrosis factor‐alpha (TNF‐α) and IL‐6 and preventing the loss in the lung architecture.ConclusionThis compound showed important anti‐inflammatory activity, with a significant ability to reduce the production of pro‐inflammatory mediators and increase the phagocytic activity of macrophages and anti‐inflammatory mediator secretion (IL‐10). These findings led us to hypothesize that this compound can repolarize the macrophage response to an anti‐inflammatory profile (M2). Moreover, it was also able to maintain its anti‐inflammatory activity in vivo experiments.

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Section: Discussionsupporting

confidence: 67%

Section: Groupsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 97%

Section: Compound Synthesismentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

Facchin,

Lubschinski,

Moon

et al. 2023

Fundamemntal Clinical Pharma

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show abstract

“…CD206, so called mannose receptor, is a membrane surface marker of M2 cells, which can specifically recognise antigens to clear pathogens, promote angiogenesis and repress the immune response [ 32 ]. FIZZ1 is also known as resistin-like molecule α, which participates in killing bacteria, viruses and other pathogens by regulating cellular immunity and promotes angiogenesis, tissue repair and wound healing [ 33 ]. Another M2-type anti-inflammatory factor, IL-10, on the one hand, enhances the sensitivity of macrophages to IL-4 and IL-13 by increasing the abundance of IL-4 receptors on the macrophage surface, which contributes to M2-type polarisation of macrophages.…”

Section: Discussionmentioning

confidence: 99%

Luteolin transforms the polarity of bone marrow-derived macrophages to regulate the cytokine storm

Wang

Zhou

et al. 2021

J Inflamm

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Background Macrophages are indispensable regulators of inflammatory responses. Macrophage polarisation and their secreted inflammatory factors have an association with the outcome of inflammation. Luteolin, a flavonoid abundant in plants, has anti-inflammatory activity, but whether luteolin can manipulate M1/M2 polarisation of bone marrow-derived macrophages (BMDMs) to suppress inflammation is still unclear. This study aimed to observe the effects of luteolin on the polarity of BMDMs derived from C57BL/6 mice and the expression of inflammatory factors, to explore the mechanism by which luteolin regulates the BMDM polarity. Methods M1-polarised BMDMs were induced by lipopolysaccharide (LPS) + interferon (IFN)-γ and M2-polarisation were stimulated with interleukin (IL)-4. BMDM morphology and phagocytosis were observed by laser confocal microscopy; levels of BMDM differentiation and cluster of differentiation (CD)11c or CD206 on the membrane surface were assessed by flow cytometry (FCM); mRNA and protein levels of M1/M2-type inflammatory factors were performed by qPCR and ELISA, respectively; and the expression of p-STAT1 and p-STAT6 protein pathways was detected by Western-blotting. Results The isolated mouse bone marrow cells were successfully differentiated into BMDMs, LPS + IFN-γ induced BMDM M1-phenotype polarisation, and IL-4 induced M2-phenotype polarisation. After M1-polarised BMDMs were treated with luteolin, the phagocytosis of M1-polarized BMDMs was reduced, and the M1-type pro-inflammatory factors including IL-6, tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), and CD86 were downregulated while the M2-type anti-inflammatory factors including IL-10, IL-13, found in inflammatory zone (FIZZ)1, Arginase (Arg)1 and CD206 were upregulated. Additionally, the expression of M1-type surface marker CD11c decreased. Nevertheless, the M2-type marker CD206 increased; and the levels of inflammatory signalling proteins phosphorylated signal transducer and activator of transcription (p-STAT)1 and p-STAT6 were attenuated and enhanced, respectively. Conclusions Our study suggests that luteolin may transform BMDM polarity through p-STAT1/6 to regulate the expression of inflammatory mediators, thereby inhibiting inflammation. Naturally occurring luteolin holds promise as an anti-inflammatory and immunomodulatory agent.

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Nifedipine attenuates alveolar bone destruction and improves trabecular microarchitectures in mice with experimental periodontitis

Lee

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Effect of nifedipine, a calcium channel blocker, on the generation of nitric oxide and interleukin-1β by murine macrophages activated by lipopolysaccharide from Prevotella intermedia

Cited by 8 publications

References 66 publications

Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

Luteolin transforms the polarity of bone marrow-derived macrophages to regulate the cytokine storm

Nifedipine attenuates alveolar bone destruction and improves trabecular microarchitectures in mice with experimental periodontitis

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