Effect of Nickel on Membrane Integrity, Lipid Peroxidation and Fatty Acid Composition in Wheat Seedlings

Abstract

The influence of 50 and 100 μm Ni on membrane integrity, hydrogen peroxide content, lipid peroxidation as well as total fatty acid composition was studied in shoots and roots of wheat seedlings. In both organs, treatment with Ni led to significant increase in electrolyte leakage reflecting the metal‐induced enhancement of membrane permeability. In the shoots, it was accompanied by significant increase in lipid peroxidation. Accumulation of hydrogen peroxide, more pronounced in the shoots, was also found in Ni‐treated wheat. Exposure of wheat seedlings to Ni altered the total fatty acid composition, leading to decrease in their unsaturation, which was indicated by reduction in double bond index. In both organs, a significant decrease in linolenic acid (C18 : 3) content was observed. It was accompanied by increased linoleic (C18 : 2) and oleic (C18 : 1) acid contents in shoots and roots, respectively. In the shoots, parallel increase in palmitic acid (C16 : 0) and decrease in palmitoleic acid (C16 : 1) were found at the end of the experiment. Our results suggest that modification of fatty acid profile in Ni‐treated wheat seedlings is partly related to lipid peroxidation and increased electrolyte leakage and may reflect damage of cells caused by the metal.

Physiological response of two brassica napus l. cultivars to nickel treatment

“…A rapid increase of TBARS in leaves with increasing Ni concentration in external solution was observed (comparing to the control). These results are in good accordance with previous studies with wheat [33] and rapeseed [28]. The difference between cultivars was evident at two highest applied metal concentrations (60 and 120 µmol · dm -3 ) and the lipid peroxidation was more pronounced in plants of cv.…”

“…In general, nickel amounts accumulated in roots of plants were higher than those of shoots, although concentrations of Ni translocated into shoots were considerably high. These findings are supported by previous experiments with wheat [33] and Solanum nigrum L. [35] plants. Dependence of accumulated amount of Ni in roots on the applied metal concentration showed quasi-parabolic course with gradual saturation of tissue by metal (both studied cultivars).…”

Characterisation of the wheat phospholipid fraction in the presence of nickel and/or selenium

“…Results for Ni-treated shoots are consistent with the observations concerning the influence of Cd, Pb and Zn on lipid metabolism in plants (Verdoni et al 2001;Nouairi et al 2006). Also, in our previous work we documented that in Ni presence the amount of C18:3 fatty acid extracted from wheat seedlings decreased significantly (Gajewska et al 2012).…”

“…In our previous papers we documented that exposure of wheat seedlings to Ni resulted in oxidative stress and changes in nitrogen and carbon metabolism Skłodowska 2007, 2009;Gajewska et al 2013). Recently we reported Ni-induced increase in membrane permeability and alterations in the total fatty acid composition, leading to a decrease in their unsaturation, which was indicated by a reduction in the double bond index (Gajewska et al 2012). To expand our knowledge on Ni toxicity to the cell membrane, in the present work we examined the influence of the metal on wheat phospholipids.…”

Plausible antioxidant biomechanics and anticonvulsant pharmacological activity of brain-targeted β-carotene nanoparticles

“…3 Protein oxidation leads to the deactivation of several enzyme systems and their associated functional changes, while lipid peroxidation causes membrane structure alterations resulting in changes in membrane fluidity, permeation, and biological functionality. 4,5 An increased calcium ion concentration in neuronal tissue leads to a cascade of biochemical responses which trigger neuronal cell death after status epilepticus. 6 High levels of intracellular calcium ions induce reactive oxygen species (ROS) in a biosystem mainly through xanthine oxidase and nicotinamide adenine dinucleotide phosphate oxidase mediated biochemical reactions.…”