Effect of new curcumin‐containing nanostructured lipid dispersions on human keratinocytes proliferative responses

Abstract

This study describes the production and characterization of nanostructured lipid dispersions (NLDs) containing curcumin (CUR) as new tools for curcumin topical delivery. Four types of NLDs based on monoolein in association with different emulsifiers were produced: Na cholate and poloxamer 407 (NLD1), poloxamer alone (NLD2), the mixture of Na cholate and Na caseinate (NLD3) and Na cholate alone (NLD4). Morphology and dimensional distribution of lipid dispersions were investigated by cryo-TEM and photon correlation spectroscopy (PCS). In vitro studies based on Franz cell, membrane nylon and stratum corneum-epidermis (SCE) were carried out to compare the four NLDs in terms of cytotoxicity in human keratinocytes and CUR diffusion. Our PCS studies showed differences in particles diameter among the different NLDs. In addition, cytotoxicity results in HaCaT cells evidenced that NLD1 and NLD2 were toxic at doses over 1 μm. Therefore, cryo-TEM was determined only for NLD3 and NLD4 showing that CUR did not affect their structure. Diffusion measurement in SCE and nylon membrane evidenced that CUR had a time-delayed release for NLD4. The 'wound healing' effect of NLD3 and NLD4 with and without CUR analysed keratinocytes in vitro, and a clear inhibition of cell proliferation/migration by CUR was observed. This effect was mediated by the inhibition of cyclin D1 expression as a consequence of the impaired NFkB activation. This study confirms the antiproliferative properties of CUR and evidenced a new possible model of CUR topical delivery for hyperproliferative cutaneous diseases such as psoriasis.

Ethosomes for Coenzyme Q10 Cutaneous Administration: From Design to 3D Skin Tissue Evaluation

“…For LDH assay seeded cells were treated for 24 h with the different vehicles at various concentrations. Cytotoxicity was evaluated by spectrophotometric quantification of the LDH released in culture medium, using a commercial kit (Sigma-Aldrich, Merck, Darmstadt, Germany), as previously described [ 36 , 37 ]. For MTT assay seeded cells were exposed to the selected formulations for 24 h, after complete removal of the treatment, 50 μL of serum-free media and 50 μL of MTT (0.5 mg/mL) were added and incubated for 3 h. The MTT was reduced in living cells, forming insoluble purple formazan crystals that were then dissolved in 100 μL of DMSO at 37 °C for 15 min.…”

Polyester-Based Dendrimer Nanoparticles Combined with Etoposide Have an Improved Cytotoxic and Pro-Oxidant Effect on Human Neuroblastoma Cells

“…Values <5 mV may lead to agglomeration of particles conferring physical instability in solution. On the contrary, Z-potential >30 mV, either positive or negative, leads to monodispersity and good physical stability in solution [45,46] Finally, high positive Z-potential values are correlated to high cytotoxicity while negative values allow high cell viability [47]. In this regards, the CPX 5 nanoparticles that proved to possess a Z-potential of −45 mV ± SD can be rationally considered stable in water solution, with no tendency to form aggregates and are endowed with low cytotoxicity.…”

“…Nanoparticles are extensively inspected to prepare multifunctional smart materials [26][27][28][29][30] and are widely employed in various biomedical applications [31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49].…”

The Neuroprotective Effect of Hericium erinaceus Extracts in Mouse Hippocampus after Pilocarpine-Induced Status Epilepticus

“…There are two types of lipid nanoparticles called solid lipid nanoparticles and nanostructured lipid carriers, depending on the function of the lipids forming nanoparticle matrix [45]. By mixing lyotropic lipid, monoolein, with assorted emulsifiers and water, various types of liquid crystalline nanocarriers can be generated [46,47,48,49,50]. Different materials can also be used to encapsulate target compounds in lipid vehicles for improving delivery efficiencies or the drug release in a controlled fashion [51,52,53].…”