Effect of new carbonyl cyanide aromatic hydrazones on biofilm inhibition against methicillin resistant <i>Staphylococcus aureus</i>

Lu, Xueer; Zhang, Ziwen; Xu, Yingying; Lü, Jun; Tang, Wenjian; Zhang, Jing

doi:10.1039/d0ra03124k

Cited by 12 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbonyl cyanide m-chlorophenylhydrazone (CCCP), a representative uncoupler, shows antibacterial activity and biofilm inhibition in S. aureus. 49 Fundamentally, Streptococci. (gram-positive) have a thick electron non-conductive cell wall and exhibit weak extracellular electron translocation properties.…”

Section: ■ Discussionmentioning

confidence: 99%

Bovine Colostrum Exosomes Are a Promising Natural Bacteriostatic Agent against Staphylococcus aureus

et al. 2023

View full text Add to dashboard Cite

Bioactive molecules and immune factors in the bovine colostrum (BC) are important elements of passive immunity that prevent bacterial infection. However, the mechanisms underlying the antimicrobial activity of BC are not fully understood. We assessed the antibacterial properties of BC-derived exosomes (BC-Exo) and found that they had bacteriostatic, anti-hemolytic, and biofilm-eradication effects on Staphylococcus aureus. Moreover, cell surface deformation and reduced ATP production were observed following BC-Exo treatment. The most reasonable explanation for this finding is that BC-Exo has a strong inhibitory effect on the oxidative phosphorylation pathway in S. aureus. We demonstrated, for the first time, that BC-Exo can exhibit clear antimicrobial activity against S. aureus. Our findings constitute an important basis for future antibiotic discovery.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Bovine Colostrum Exosomes Are a Promising Natural Bacteriostatic Agent against Staphylococcus aureus

et al. 2023

View full text Add to dashboard Cite

show abstract

“…12 The efflux pumps also play a role in biolm formation. 13 The inhibition of efflux pumps helps to enhance the therapeutic efficacy of traditional antibiotics by affecting QS genes and virulence factors to control biolm production. 11 Recently, CCCP analog 2e was found to display alone or synergistic efficacy against MRSA.…”

Section: Introductionmentioning

confidence: 99%

“…11 Recently, CCCP analog 2e was found to display alone or synergistic efficacy against MRSA. 13 2e could inhibit biolm formation, effectively eradicated preformed biolm, and showed low toxicity for human hepatic L02 cells. 14 Studying the anti-biolm activity of 2e and 2e combined with ooxacin will help to understand the role and relationship of efflux pump and biolm formation in MRSA.…”

Section: Introductionmentioning

confidence: 99%

Efflux pump inhibitor combined with ofloxacin decreases MRSA biofilm formation by regulating the gene expression of NorA and quorum sensing

Wang

Xie

et al. 2023

RSC Adv.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biofilm formation and dispersal are highly regulated by quorum sensing (QS) at the genetic level ( Lu et al, 2020 ; Attallah et al, 2022 ). Adherence-related virulence factors include esp -encoded surface protein, agg -encoded aggregation substance ( Soares et al, 2014 ; Spiegelman et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and anti-biofilm activity of diarylureas against Enterococcus faecium by suppressing the gene expression of peptidoglycan hydrolases and adherence

et al. 2022

Self Cite

View full text Add to dashboard Cite

Enterococcus faecium (E. faecium) is a clinical multidrug-resistant pathogen causing life-threatening infection, which makes it important to discover antibacterial agents with novel scaffolds and unique mechanism. In this study, the diarylurea scaffold was found to have potent antibacterial effect on E. faecium. Diarylurea ZJ-2 with benign drug-like property exhibited potent antibacterial and anti-biofilm activity through inhibiting the genes expression of NlpC/p60 hydrolase-secreted antigen A (sagA) and autolysins (atlA), down-regulating the expression of biofilm adherence related genes aggregation substance (agg), enterococcal surface protein (esp) against E. faecium. Moreover, ZJ-2 can be docked into SagA to inhibit daughter cell separation. In a mouse model of abdominal infection, ZJ-2 decreased the bacterial load and the level of IL-6 and TNF-α in a time-dependent manner. Overall, these findings indicated that diarylurea ZJ-2 has the potential to be developed as a therapeutic agent to treat drug-resistant enterococci and biofilm-related infections.

show abstract

Effect of new carbonyl cyanide aromatic hydrazones on biofilm inhibition against methicillin resistant Staphylococcus aureus

Abstract: 2e and 2j with strong p-NO2 and p-CF3 at phenyl ring had the lowest MICs against S. aureus and MRSA. 2e displayed unaided or synergistic efficacy against MRSA, especially combined with ofloxacin. EM revealed that 2e destroys biofilms and cell membranes.

Cited by 12 publications

References 27 publications

Bovine Colostrum Exosomes Are a Promising Natural Bacteriostatic Agent against Staphylococcus aureus

Bovine Colostrum Exosomes Are a Promising Natural Bacteriostatic Agent against Staphylococcus aureus

Efflux pump inhibitor combined with ofloxacin decreases MRSA biofilm formation by regulating the gene expression of NorA and quorum sensing

Antibacterial and anti-biofilm activity of diarylureas against Enterococcus faecium by suppressing the gene expression of peptidoglycan hydrolases and adherence

Contact Info

Product

Resources

About