Effect of neonatal or adult heat acclimation on testicular and epididymal morphometry and sperm production in rats

Kurowicka, Beata; Dietrich, G.J.; Kotwica, Genowefa

doi:10.1016/j.repbio.2015.01.001

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…The results of our present and previous studies (Kurowicka et al 2015b) indicate differentiated impact of heat acclimation on testicular function despite similar plasma fT3 concentrations found in AHA and NHA rats. Therefore, in further in vitro experiments, we aimed to delineate T3 effects on the testicular steroidogenesis at the molecular level in heat-acclimated rats.…”

Section: Discussionsupporting

confidence: 76%

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Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Kurowicka¹,

Chruściel²,

Zmijewska³

et al. 2016

Polish Journal of Veterinary Sciences

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This study aimed to evaluate the effect of heat acclimation of neonatal and adult rats on their testes response to in vitro treatment with triiodothyronine (T3). Four groups of rats were housed from birth as: 1) control (CR) at 20 C. Blood plasma and both testes were harvested from 90-day old rats. Testicular slices were then submitted to in vitro treatment with T3 (100 ng/ml) for 8 h. Plasma fT3 level was lower in AHA, NHA and DA groups than in CR group. Basal thyroid hormone receptor α1 (Thra1) expression was higher in testes of NHA and DA and β1 receptor (Thrb1) in DA rats vs. other groups. In the in vitro experiment, T3: 1) decreased Thra1 expression in all groups and Thrb1 in DA group, 2) increased Star expression in CR, NHA and DA groups, and Hsd17b3 expression in NHA group, 3) decreased the expression of Cyp11a1 in NHA and DA groups, and Cyp19a1 in all the groups, 4) did not affect the activity of steroidogenic enzymes and steroid secretion (A4, T, E2) in all the groups. These results indicate, that heat acclimation of rats, depending on their age, mainly affects the testicular expression of steroidogenic enzymes in response to short-lasting treatment with T3.

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Section: Discussionsupporting

confidence: 76%

“…We have also reported severe histological changes in the testes and abnormal sperm production mainly in adult heat-acclimated males (Kurowicka et al 2015b).…”

Section: Discussionmentioning

confidence: 60%

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Kurowicka¹,

Chruściel²,

Zmijewska³

et al. 2016

Polish Journal of Veterinary Sciences

Self Cite

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“…Morphometric analysis of testes revealed that the tubular diameters of the seminiferous tubules at both ages were significantly reduced in the betamethasone-group. Tubular diameter is a sensitive indicator of spermatogenic capacity of several species of animals [53,54]. It is possible that the betamethasone treatment reduced sperm production.…”

Section: Discussionmentioning

confidence: 99%

Alterations in male rats following in utero exposure to betamethasone suggests changes in reproductive programming

Borges

Dias

Rosa

et al. 2016

Reproductive Toxicology

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Antenatal betamethasone is used for accelerating fetal lung maturation for women at risk of preterm birth. Altered sperm parameters were reported in adult rats after intrauterine exposure to betamethasone. In this study, male rat offspring were assessed for reproductive development after dam exposure to betamethasone (0.1mg/kg) or vehicle on Days 12, 13, 18 and 19 of pregnancy. The treatment resulted in reduction in the offspring body weight, delay in preputial separation, decreased seminal vesicle weight, testosterone levels and fertility, and increased testicular weight. In the testis, morphologically abnormal seminiferous tubules were observed, characterized by an irregular cell distribution with Sertoli cell that were displaced towards the tubular lumen. These cells expressed both Connexin 43 (Cx43) and Proliferative Nuclear Cell Antigen (PCNA). In conclusion, intrauterine betamethasone treatment appears to promote reproductive programming and impairment of rat sexual development and fertility due to, at least in part, unusual testicular disorders.

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“…In addition, the seminiferous epithelium in ipsilateral testis of varicocelized rats also had lower measures than the sham group. In this study, testicular temperature was not determined; however, we believe that hyperthermia, caused by venous stasis, is the probable cause of these alterations since long-term exposure to high temperatures can impair testicular morphometry [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Chrysin Administration Protects against Oxidative Damage in Varicocele‐Induced Adult Rats

Missassi

Borges

Rosa

et al. 2017

Oxidative Medicine and Cellular Longevity

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Oxidative stress is known as the leading factor responsible for varicocele-related infertility and for that reason, many antioxidant therapies have been proposed. Considering that, we evaluated the reproductive outcomes and fertility of varicocelized rats and the impact of chrysin within these parameters. The animals were allocated into three groups: sham (control), varicocele treated via gavage with 50 mg/kg/day of chrysin (V1), or vehicle (V2) for 56 days. Chrysin treatment prevented oxidative damage resulting from varicocele by decreasing testicular concentrations of malondialdehyde and sperm DNA fragmentation. It also improved histological aspect of the testis and maintained morphometric parameters similar to the sham group. Furthermore, there were no differences in body and reproductive organ weights, histopathological analysis of epididymis, sperm counts and morphology, testosterone levels, sexual behavior, and fertility parameters among experimental groups. Our results reinforce the idea that injuries provoked by experimental varicocele are related, at least in part, to oxidative stress. Moreover, varicocele showed bilateral deleterious effects without interfering with fertility. Chrysin administration significantly ameliorated sperm parameters, protecting the reproductive system against varicocele damages. For that reason, chrysin might be an alternative adjuvant therapy to improve sperm quality in men presenting this condition.

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Effect of neonatal or adult heat acclimation on testicular and epididymal morphometry and sperm production in rats

Cited by 5 publications

References 27 publications

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Alterations in male rats following in utero exposure to betamethasone suggests changes in reproductive programming

Chrysin Administration Protects against Oxidative Damage in Varicocele‐Induced Adult Rats

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