Effect of natural betaine on estimates of semen quality in mature AI boars during summer heat stress

Cabezón, F. A.; Stewart, Kara R; Schinckel, A. P.; Barnes, Will C.; Boyd, R. D.; Wilcock, P.; Woodliff, Jeffrey

doi:10.1016/j.anireprosci.2016.03.009

Cited by 23 publications

(15 citation statements)

References 23 publications

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“…Bet has also been found to improve tolerance to stress [9,13], but studies on the reproduction of roosters remain limited. However, a study conducted in boars is available [14], which indicates that dietary 0.63 and 1.26% Bet elevated the amount of sperm compared to the control animals. Adding 200 mg/kg of Bet to the diet decreased the mortality of heat-stressed chickens; enhanced their immunity and health status [15]; and increased their antioxidant status, such as glutathione peroxidase (GPx) and superoxide dismutase (SOD), while decreasing malondialdhyde (MDA) in blood serum [16] and in breast muscle tissue [17].…”

Section: Introductionmentioning

confidence: 99%

Effect of Supplementation with Trimethylglycine (Betaine) and/or Vitamins on Semen Quality, Fertility, Antioxidant Status, DNA Repair and Welfare of Roosters Exposed to Chronic Heat Stress

Attia

El-Naggar

Abou-Shehema

et al. 2019

Animals

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In this study, we investigated the influence of betaine (Bet, 1000 mg/kg), with or without vitamin C (VC, 200 mg/kg ascorbic acid) and/or vitamin E (VE, 150 mg/kg α-tocopherol acetate) on semen quality, seminal and blood plasma constituents, antioxidants’ status, DNA repair, and the welfare of chronic heat stress (CHS)-exposed roosters. A total of 54 roosters were divided into six groups of nine replicates. One group was kept under thermoneutral conditions, whereas the other five were kept under CHS. One of the five groups served as an unsupplemented CHS group, and was fed with a basal diet. The other four CHS groups were supplemented with Bet, Bet + VC, Bet + VE, and Bet + VC + VE, respectively. Our data indicate that supplementation with Bet, Bet + VC, Bet + VE, and Bet + VC + VE, resulted in complete recovery of the CHS effect on sperm concentration and livability, semen pH, and fertility compared to the thermoneutral group. Seminal plasma total antioxidant capacity (TAC) was significantly (p < 0.05) increased with Bet, with or without vitamins, compared to the thermoneutral and CHS groups. Urea and blood plasma malondialdehyde (MDA) were totally recovered with Bet, with or without vitamin treatments. Both the jejunum and ileum DNA were partially recovered following Bet, with or without vitamin supplementation. In conclusion, Bet, at 1000 mg/kg feed, may be a useful agent for increasing semen quality, fertility, welfare, and to improve the breeding strategy of breeder males in hot climates.

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Section: Introductionmentioning

confidence: 99%

Effect of Supplementation with Trimethylglycine (Betaine) and/or Vitamins on Semen Quality, Fertility, Antioxidant Status, DNA Repair and Welfare of Roosters Exposed to Chronic Heat Stress

Attia

El-Naggar

Abou-Shehema

et al. 2019

Animals

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“…Tail abnormalities increased with high levels (205 mM) of betaine supplementation in the present study. Cabezon et al (2016) demonstrated that boars fed diets containing 1.26% betaine had a higher incidence of DMRs compared with 0.63% betaine but were not different from the nonsupplemented controls. The induced tail abnormalities and reduced motility seen in Exp1 with 205 mM betaine supplementation were likely caused by the high solute content of the extender, which likely caused alterations in cell volume, leading to membrane damage ( Du et al, 1994 ; Gilmore et al, 1996 ).…”

Section: Resultsmentioning

confidence: 89%

“…The differences in these two studies are likely due to the naturally present betaine in the boar’s seminal plasma. Cabezon et al (2016) showed that not only is betaine present and variable in boar seminal plasma, but also that supplementing betaine into the diets of boars increases the betaine concentration in the seminal plasma. The boars used in Exp1 had differing genetics and were 6 mo older than those in Exp2.…”

Section: Resultsmentioning

confidence: 99%

“…Exp1 demonstrates that betaine supplementation may alleviate some of the dilution stress on the sperm, as motility was not significantly reduced until the brix score was at 6.7 and was increased when the brix score was at 5.7. Diet supplementation of betaine to boars has been shown to increase seminal plasma concentration of betaine, though no significant differences were detected in total motility or progressive motility ( Cabezon et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

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Effects of supplemental betaine to semen extenders on semen quality in boars

Lugar

Krom

Mings

et al. 2018

Translational Animal Science

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Two experiments were conducted to evaluate the use of supplemental betaine in commercially available semen extenders. In experiment 1 (Exp1), semen was collected from six mature boars once weekly for 6 wk (3 wk in summer and 3 wk in winter) and diluted into a commercial extender with the following betaine concentrations: 0, 51, 102, and 205 mM. Semen samples were analyzed on the day of collection (D0) and after 72 h of storage (D3). In experiment 2 (Exp2), semen was collected from four mature boars for 3 wk and was diluted into three commercially available semen extenders (short term, ST; long term with bovine serum albumin, BSA; and long term without BSA, LT), with and without supplemental betaine (0 and 70 mM), and analyzed on D0 and D3. Semen was analyzed using computer-assisted sperm assessment (Ceros II, IMV) and morphology using phase contrast microscopy. In Exp1, total motility on D0 was less for 0 mM than that for 102 mM (P = 0.038) and was substantially reduced for 205 mM compared with 102 mM (P < 0.001). Supplementation with 205 mM betaine resulted in a significant reduction in the percentage of morphologically normal sperm (P < 0.001). In Exp2, 70 mM betaine reduced the total motility compared with 0 mM (P = 0.010) but did not impact percentage of normal sperm (P = 0.942). The use of supplemental betaine may partially alleviate the dilution effect on sperm, though boar genetics may impact its efficacy. Further research is needed to make a definitive conclusion.

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“…As an osmolyte, in order to maintain fluid balance, betaine can protect cells, enzymes and proteins from environmental stresses, including high salinity and extreme temperatures. As a methyl donor, betaine is involved in the methionine cycle in the kidneys and liver in humans (8). In addition, betaine participates in a variety of biological processes.…”

Section: Introductionmentioning

confidence: 99%

Betaine alleviates high glucose‑induced mesangial cell proliferation by inhibiting cell proliferation and extracellular matrix deposition via the AKT/ERK1/2/p38 MAPK pathway

Wang

2019

Mol Med Report

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Diabetic nephropathy (DN) is a major cause of chronic renal failure in diabetic patients worldwide. Betaine, a zwitterionic quaternary ammonium salt compound, is involved in numerous biological processes. The present study aimed to investigate the effects of betaine on mouse mesangial cells (MMCs) cultured under high glucose (HG) conditions and its underlying mechanisms. MMCs were treated with betaine under HG conditions. Cell proliferation and the cell cycle distribution were investigated with an MTT assay and flow cytometry, respectively. Western blotting and reverse transcription-quantitative polymerase chain reaction analyses were applied to respectively determine protein and mRNA expression levels. The results suggested that betaine decreased cell proliferation in a dose-dependent manner, while G1-phase arrest was significantly induced in MMCs. Compared with the control group, the expression levels of p21 and p27 decreased under HG conditions, but were reversed by betaine. Furthermore, the expression levels of fibronectin and type IV collagen were significantly decreased in cells treated with betaine compared with the HG group. Additionally, betaine decreased the phosphorylation of Akt, extracellular-signal-regulated kinase (Erk)1/2 and p38 mitogen-activated protein kinase (MAPK), but was enhanced under HG conditions. Overall, the results of the present study indicated that betaine serves a protective role in HG-induced MMCs by inhibiting cell proliferation and extracellular matrix deposition via regulating regulation of the Akt/Erk1/2/p38 MAPK signaling pathway.

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Effect of natural betaine on estimates of semen quality in mature AI boars during summer heat stress

Cited by 23 publications

References 23 publications

Effect of Supplementation with Trimethylglycine (Betaine) and/or Vitamins on Semen Quality, Fertility, Antioxidant Status, DNA Repair and Welfare of Roosters Exposed to Chronic Heat Stress

Effect of Supplementation with Trimethylglycine (Betaine) and/or Vitamins on Semen Quality, Fertility, Antioxidant Status, DNA Repair and Welfare of Roosters Exposed to Chronic Heat Stress

Effects of supplemental betaine to semen extenders on semen quality in boars

Betaine alleviates high glucose‑induced mesangial cell proliferation by inhibiting cell proliferation and extracellular matrix deposition via the AKT/ERK1/2/p38 MAPK pathway

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