Effect of Naphthalene Acetic Acid (NAA) on in vitro rooting of regenerated microshoots of groundnut (<i>Arachis hypogaea</i> L.)

Abstract

Successful utilization of tissue culture for the improvement and conservation of groundnut will be made possible only with an efficient shoot proliferation, formation of well-developed root system in microshoots, successful acclimatization and final establishment in field. A regeneration protocol ensuring a high frequency rooting of microshoots derived from embryonic axes of four groundnut genotypes was achieved using naphthalene acetic acid (NAA). Microshoots of 3cm length were subcultured on Murashige and Skoog (MS) medium plus 30% sucrose, supplement with 1.0 or 1.5mg/L NAA and compared with a hormone free medium (control). Rooting was significantly influenced by the presence and concentration of NAA. Microshoots subcultured in medium supplemented with 1mg/L NAA rooted within 2weeks of subculture, producing the highest number of roots/plantlet and root-induction frequency. However, increasing the concentration of the auxin suppressed root length with the hormone free medium having the longest roots. Significant genotypic effect was also observed, with Samnut 22 having more number of roots/plantlet and root-induction frequency.

Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) Immersed in Liquid Nitrogen

“…The observed differences among the genotypes could be due to genotypic influence, as tissue culture response in groundnut is strongly influenced by the plant genotype [22,23]. Genotypic influence has also been reported in cryopreserved embryonic axes of maize [24]. There was a significant interaction between the genotypes and time of desiccation for moisture content of cryopreserved embryonic axes of groundnut.…”

In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

“…It is shown that the regeneration protocol improved by this experiment is more proper for commercial use than established report (Singh et al, 2009) because of minimum use of hormone concentration. An efficient shoot proliferation is made possible with a well-developed rooting system for successful acclimatization and finally it can be established in field (Abdulmalik et al, 2012). Mostly, NAA and IAA were used in different concentrations in full and 1/2 strength of MS basal medium (Fotopoulos and Sotiropoulos, 2005).…”

Micropropagation of Yucca Plant by Using Guar and Locust Bean Seed Powder as an Alternative Cheap Gelling Agent

“…Moreover, Abdulmalik et al (2012) on Arachis hypogaea L. reported that rooting was significantly influenced by the presence and concentration of NAA. Microshoots subcultured on medium supplemented with 1 mg l -1 NAA rooted within 2 weeks of subculture, producing the highest number of roots/plantlet and root-induction frequency.…”