Effect of nanoscale bioactive glass with radial spherical particles on osteogenic differentiation of rat bone marrow mesenchymal stem cells

Wang, Lili; Yan, Jia; Hu, Xiaokun; Zhu, Xinchen; Hu, Shuying; Qian, Jun; Zhang, Feimin; Liu, Mei

doi:10.1007/s10856-020-06368-8

Cited by 9 publications

(4 citation statements)

References 79 publications

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“…The shCtrl or shAnxA6 MC3T3-E1 cells were seeded onto slides or 6-well plates at a density of 5 × 10 5 cells/mL, cultured with osteogenic medium for 5 days [ 56 , 57 ], and then lysed with a cell lysis solution (free of phosphatase inhibitors) for 30 min. The lysis solution was collected and centrifuged at 14,000× g rpm for 10 min, followed by determining the ALP activity of the supernatant with an Alkaline Phosphatase Assay Kit (P0321S, Beyotime, Shanghai, China) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells

Pei

Yang

et al. 2022

IJMS

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Fluid shear stress (FSS) facilitates bone remodeling by regulating osteogenic differentiation, and extracellular matrix maturation and mineralization. However, the underlying molecular mechanisms of how mechanical stimuli from FSS are converted into osteogenesis remain largely unexplored. Here, we exposed MC3T3-E1 cells to FSS with different intensities (1 h FSS with 0, 5, 10, and 20 dyn/cm2 intensities) and treatment durations (10 dyn/cm2 FSS with 0, 0.5, 1, 2 and 4 h treatment). The results demonstrate that the 1 h of 10 dyn/cm2 FSS treatment greatly upregulated the expression of osteogenic markers (Runx2, ALP, Col I), accompanied by AnxA6 activation. The genetic ablation of AnxA6 suppressed the autophagic process, demonstrating lowered autophagy markers (Beclin1, ATG5, ATG7, LC3) and decreased autophagosome formation, and strongly reduced osteogenic differentiation induced by FSS. Furthermore, the addition of autophagic activator rapamycin to AnxA6 knockdown cells stimulated autophagy process, and coincided with more expressions of osteogenic proteins ALP and Col I under both static and FSS conditions. In conclusion, the findings in this study reveal a hitherto unidentified relationship between FSS-induced osteogenic differentiation and autophagy, and point to AnxA6 as a key mediator of autophagy in response to FSS, which may provide a new target for the treatment of osteoporosis and other diseases.

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Section: Methodsmentioning

confidence: 99%

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells

Pei

Yang

et al. 2022

IJMS

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“…Furthermore, based on the pictures taken from this sample after 14 ( Figure 6b) and 28 days (Figure 6c) of soaking in the SBF solution, it is observed that hydroxyapatite clusters were formed on the sample. It can be proven that bioactive glass with spherical particles exhibits superior biological activity due to regular shape [40]. Also, it can be seen that more clusters were present on the surface of the sample after 28-day immersion.…”

Section: Surface Morphology Analysismentioning

confidence: 85%

“…Because of nanobioactive glasses have a higher speci c surface area compared to micro-sized, from DLS test, it will expect that the synthesized composites will show good biological properties [40].…”

Section: Particle Size Analysismentioning

confidence: 99%

Bioactive Nanocomposites Composed of Magnesium Oxide Nanoparticles and 58S Bioactive Glass: Synthesis, Characterization, Bioactivity Evaluation and Antibacterial Activity

Zaferani¹,

Nabian²,

Rabiee³

2020

Preprint

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Abstract One of the main strategies to facilitate bone defect healing is the application of a variety of biological materials such as ceramic-based implants and scaffolds. Bioactive glasses are recognized as the favorable biomaterials in bone tissue engineering since they can quickly form a bond with both hard and soft tissues. In this study, novel bioactive nanocomposites based on 58S bioactive glass in composite with various weight percent of magnesium oxide nanoparticles were prepared. DLS measurements represented that 98.1% of 58S glass particles possessed the particle size between 70 and 200 nm. The bioactivity of nanocomposite powders was evaluated through immersion of samples in the simulated body fluid (SBF) at different time intervals of 14 and 28 days. Moreover, the nanocomposite samples were characterized in terms of morphology, phase structure and functional groups using Scanning electron microscopy (SEM), X-ray diffraction and Fourier transform infrared spectroscopy (FTIR) before and after soaking in the SBF solution. It was found that hydroxyapatite was formed on the surface of all nanocomposites after soaking in the SBF solution, although they bioactivity decreased with an increase in the amount of MgO nanoparticles from 15 wt. % to 25 wt. %. Moreover, antibacterial activity of the produced nanocomposites against MRSA bacteria was investigated and the results showed that 58S-15Mg exhibited the highest bactericidal activity.

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“…Cu incorporation at Na site drastically affected the process conditions and leads to the formation of smaller faceted particles and it is agglomerated due to the process of abnormal reduction rate of super-saturation and fast supply of ions influencing the growth interface. Wang et al [55] recently reported the spherical and radial spherical nano-sized particles of BAG and explained that, while varying the concentration and composition in sol-gel chemistry, influences the morphological growth kinetics. Similarly, in our previous report [57], the morphological aspects of bioactive materials at different temperatures were elaborated.…”

Section: Surface Properties Of Bag and Cu-bagmentioning

confidence: 99%

Unravelling the effects of ibuprofen-acetaminophen infused copper-bioglass towards the creation of root canal sealant

Chitra¹,

Chandran²,

Ramadoss³

et al. 2022

Biomed. Mater.

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Impact towards the tuneable characteristics of bioactive glasses (BAG) has been explored; as there is no root canal sealant till date with ideal characteristics competent enough to manoeuvre the perplexing root canal architecture. Combeite, calcite and traces of cuprorivaite crystalline phases were validated for material formation, in which Cu 2P [XPS] peak authenticating the presence of copper in bioglass network (Cu-BAG). Spherical and platelet-like morphologies were observed and the grain size of Cu-BAG (~100 nm) was lesser as compared to BAG (~ 1 µm). These particle distributions impacted the porosity, and dominant non-bridging oxygens in Cu-BAG influences ionic dissolution, which subsequently enhanced the mineralization. These bioactive materials were loaded with acetaminophen and ibuprofen, corresponding organic moieties was confirmed through FT-IR. These drugs loaded bioactive materials exhibited tremendous anti-inflammatory and anti-microbial behavior with better sealing ability. Drug loaded bioglass paste filled in biomechanically prepared root canal was estimated for sealing potential, mineralization, micro leakage, and fracture resistance properties. Hydroxyl apatite growth was noted on the sealants, flower like protuberance confirmed the sealing potential of the prepared material. Bioglass exhibited promising characteristics required in a root canal sealant. This investigation is a step further towards tailoring the properties of bioactive materials as promising candidates in root canal obturation and thereof.

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Effect of nanoscale bioactive glass with radial spherical particles on osteogenic differentiation of rat bone marrow mesenchymal stem cells

Cited by 9 publications

References 79 publications

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells

Bioactive Nanocomposites Composed of Magnesium Oxide Nanoparticles and 58S Bioactive Glass: Synthesis, Characterization, Bioactivity Evaluation and Antibacterial Activity

Unravelling the effects of ibuprofen-acetaminophen infused copper-bioglass towards the creation of root canal sealant

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