Effect of Nanoreduction on Functional and Structural Properties of Resistant-Starch from Lotus Stem

Abstract

This study characterizes the resistant starch (RS) and ball milled RS nanoparticles extracted from the stem of Nelunmbo nucifera, an underutilized aquatic plant. Starch (NS) was extracted from lotus stem and subjected to enzymatic digestion to isolate naturally present RS. The RS nanoparticles were produced via planetary ball milling carried out for 30 min (RS30), 60 min (RS60), and 90 min (RS90). The samples so obtained were characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM), light microscopy, Fourier transform infra-spectroscopy (ATR-FTIR), X-ray diffraction (XRD), and differential scanning colorimeter (DSC). DLS revealed a hydrodynamic diameter of 12.