Effect of nanoparticulate polybutylcyanoacrylate and methylmethacrylate–sulfopropylmethacrylate on the permeability of zidovudine and lamivudine across the in vitro blood–brain barrier

Kuo, Yung‐Chih; Chen, Hung-Hao

doi:10.1016/j.ijpharm.2006.07.044

Cited by 103 publications

(41 citation statements)

References 40 publications

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“…Thus, protection in physiological environment and increase of residence time of lamivudine inside cells would greatly improve the treatment efficiency. To achieve this aim, lamivudine has been encapsulated into various colloidal carriers such as biodegradable polymer spheres [7] and micelles [8], and conjugated to polysaccharide and dendrimers [9,10].…”

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confidence: 99%

Preparation and cellular uptake of PLGA particles loaded with lamivudine

et al. 2012

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Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles loaded with lamivudine and coated with bovine serum albumin (BSA) were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, concentration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtaining PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of ~35%. The particles were observed by scanning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.PLGA, lamivudine, cell uptake, intracellular distribution, drug delivery Citation:Wang B, Chen G Q, Mao Z W, et al. Preparation and cellular uptake of PLGA particles loaded with lamivudine.

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Preparation and cellular uptake of PLGA particles loaded with lamivudine

et al. 2012

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“…Polysorbate 80 has been found to increase the translocation of nanoparticles by increasing the particle interaction with the low density lipoprotein (LDL) receptor-mediated endocytic pathway in brain endothelial cells and by inhibition the efflux function of P-gp (Goppert & Muller, 2005). Kuo and Chen (2006) showed that methylmethacrylate-sulfopropylmethacrylate (MMSPM) nanoparticles were able to significantly increase the BBB permeability of zidovudine and lamivudine by 100%, using blood-brain-microvascular endothelial cells model. In the same study, PBCA nanoparticles increased the BBB permeability of zidovudine 8-to 20-fold and lamivudine 10-to 18-fold.…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

Exploring the Nanotechnology-Based Drug Delivery Systems for AIDS Treatment

Carvalho¹,

Mainardes²,

Gremião³

2011

Understanding HIV/AIDS Management and Care - Pandemic Approaches in the 21st Century

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“…The correlation of drug loading with another essential property of nanoparticles, that is, drug release profiles would have been useful in this study. By using an in vitro blood brain-microvascular endothelial cells model (BMEC), Kuo and Chen (2006) then evaluated the effect of size of PBCA and MMA-SPM nanoparticles and alcohol on the permeability of AZT and lamivudine (3TC) across the blood brain barrier. It was found that the loading efficiency and permeability of AZT and 3TC decreased with an increase in the particle size of the two polymeric carriers.…”

Section: Nanoparticles For Arv Drug Therapymentioning

confidence: 99%