Effect of Nanocrystalline Particles of Chitin on Blood Components in Humans and Experimental Animals

Дрозд, Н. Н.; Торлопов, М. А.; Udoratina, E. V.; Logvinova, Yu.S.

doi:10.1007/s10517-018-4076-z

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…It has been recognized that chitin of crustacean origin induces blood coagulation by adhering to platelets, forming a chitin/platelet complex that accelerates the polymerization of the fibrin monomer to form a blood clot (for review, see [55]). Consequently, several studies on chitin in the form of nanocrystals [56] or powders [55] as a potential hemostatic biomaterial have been carried out. However, for example, a hydrosol containing nanocrystalline particles showed no effect on the aggregation of human platelets and the clotting time of platelet-poor plasma in corresponding coagulation tests [56].…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, several studies on chitin in the form of nanocrystals [56] or powders [55] as a potential hemostatic biomaterial have been carried out. However, for example, a hydrosol containing nanocrystalline particles showed no effect on the aggregation of human platelets and the clotting time of platelet-poor plasma in corresponding coagulation tests [56]. Therefore, we propose that experiments using 3D structured sponge chitins, including those from A. archeri , should be carried out to evaluate their hemostatic properties in future clinical settings.…”

Section: Resultsmentioning

confidence: 99%

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Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Klinger

Żółtowska-Aksamitowska

Wysokowski

et al. 2019

Marine Drugs

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Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Klinger

Żółtowska-Aksamitowska

Wysokowski

et al. 2019

Marine Drugs

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“…In recent years, the novel usage of chitin in the field of drug delivery has been nanocrystalline particles. In 2018, Drozd et al published an article confirming that nanoparticles constructed by chitin have no platelet aggregation or secretion when their concentration is below 0.63 mg/mL [93]. This result proved that nanocrystalline chitin has the potential to be a drug delivery system.…”

Section: Chitin Chitosan and Chitosan Oligosaccharidesmentioning

confidence: 97%

Carbohydrates Used in Polymeric Systems for Drug Delivery: From Structures to Applications

Liang

Shen

et al. 2022

Pharmaceutics

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Carbohydrates, one of the most important compounds in living organisms, perform numerous roles, including those associated with the extracellular matrix, energy-related compounds, and information. Of these, polymeric carbohydrates are a class of substance with a long history in drug delivery that have attracted more attention in recent years. Because polymeric carbohydrates have the advantages of nontoxicity, biocompatibility, and biodegradability, they can be used in drug targeting, sustained drug release, immune antigens and adjuvants. In this review, various carbohydrate-based or carbohydrate-modified drug delivery systems and their applications in disease therapy have been surveyed. Specifically, this review focuses on the fundamental understanding of carbohydrate-based drug delivery systems, strategies for application, and the evaluation of biological activity. Future perspectives, including opportunities and challenges in this field, are also discussed.

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“…As a result, at the early stage of wounds, the chitin dressing may induce fibroblasts to produce fine type III collagen through histiocytes [66]. In fact, several studies have been carried out on chitin in the form of nanocrystals [67] or powders [64] and non-woven fabrics [65] as a potential hemostatic biomaterial. To overcome the technical limitations associated with the transformation of chitin nanopowder to fibrillar material, we propose the scientific community evaluate the hemostatic properties in future clinical settings for 3D-structured sponge chitins, including those from I. labyrinthus.…”

Section: D Chitin Scaffolds Of Poriferan Origin As Alternative Gauzementioning

confidence: 99%

Naturally Prefabricated Marine Biomaterials: Isolation and Applications of Flat Chitinous 3D Scaffolds from Ianthella labyrinthus (Demospongiae: Verongiida)

Schubert

Binnewerg

Voronkina

et al. 2019

IJMS

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Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this study, we focus our attention, for the first time, on the marine sponge Ianthella labyrinthus Bergquist & Kelly-Borges, 1995 (Demospongiae: Verongida: Ianthellidae) as a novel potential source of naturally prestructured bandage-like 3D scaffolds which can be isolated simultaneously with biologically active bromotyrosines. Specifically, translucent and elastic flat chitinous scaffolds have been obtained after bromotyrosine extraction and chemical treatments of the sponge skeleton with alternate alkaline and acidic solutions. For the first time, cardiomyocytes differentiated from human induced pluripotent stem cells (iPSC-CMs) have been used to test the suitability of I. labyrinthus chitinous skeleton as ready-to-use scaffold for their cell culture. Results reveal a comparable attachment and growth on isolated chitin-skeleton, compared to scaffolds coated with extracellular matrix mimetic Geltrex®. Thus, the natural, unmodified I. labyrinthus cleaned sponge skeleton can be used to culture iPSC-CMs and 3D tissue engineering. In addition, I. labyrinthus chitin-based scaffolds demonstrate strong and efficient capability to absorb blood deep into the microtubes due to their excellent capillary effect. These findings are suggestive of the future development of new sponge chitin-based absorbable hemostats as alternatives to already well recognized cellulose-based fabrics.

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Effect of Nanocrystalline Particles of Chitin on Blood Components in Humans and Experimental Animals

Cited by 4 publications

References 15 publications

Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Carbohydrates Used in Polymeric Systems for Drug Delivery: From Structures to Applications

Naturally Prefabricated Marine Biomaterials: Isolation and Applications of Flat Chitinous 3D Scaffolds from Ianthella labyrinthus (Demospongiae: Verongiida)

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