Effect of Nano-Selenium on Nutritional Quality of Cowpea and Response of ABCC Transporter Family

Abstract

It is an important way for healthy Selenium (Se) supplement to transform exogenous Se into organic Se through crops. In the present study, Vigna unguiculata was selected as a test material and sprayed with biological nano selenium (SeNPs) and Na2SeO3, and its nutrient composition, antioxidant capacity, total Se and organic Se content were determined, respectively. Further, the response of ABC transporter family members in cowpea to different exogenous Se treatments was analyzed by transcriptome sequencing combined with different Se forms. The results show that the soluble protein content of cowpea increased after twice Se treatment. SeNPs treatment increased the content of cellulose in cowpea pods. Na2SeO3 treatment increased the content of vitamin C (Vc) in cowpea pods. Se treatments could significantly increase the activities of Peroxidase (POD), polyphenol oxidase (PPO) and catalase (CAT) in cowpea pods and effectively maintain the activity of Superoxide dismutase (SOD). SeNPs can reduce the content of malondialdehyde (MDA) in pods. After Se treatment, cowpea pods showed a dose-effect relationship on the absorption and accumulation of total Se, and Na2SeO3 treatment had a better effect on the increase of total Se content in cowpea pods. After treatment with SeNPs and Na2SeO3, the Se species detected in cowpea pods was mainly SeMet, followed by MeSeCys. Inorganic Se can only be detected in the high concentration treatment group. Analysis of transcriptome data of cowpea treated with Se showed that ABC transporters could play an active role in response to Se stress and Se absorption, among which ABCB, ABCC and ABCG subfamilies played a major role in Se absorption and transportation in cowpea. Further analysis by weighted gene co-expression network analysis (WGCNA) showed that the content of organic Se in cowpea treated with high concentration of SeNPs was significantly and positively correlated with the expression level of three transporters ABCC11, ABCC13 and ABCC10, which means that the ABCC subfamily may be more involved in the transmembrane transport of organic Se in cells.

Transcriptome and co-expression network revealed molecular mechanism underlying selenium response of foxtail millet (Setaria italica)

“…ABC transporters have been implicated in Se accumulation and transport in rice, and were detected after SeO 3 2− treatment, suggesting their potential involvement in Se accumulation and transport ( Kong et al., 2021 ). ABCC11, ABCC13, and ABCC10 engage in nano-selenium accumulation and transport in cowpeas ( Li et al., 2023 ). ABC transporter G family member 36 in alfalfa leaves is significantly upregulated after Se treatment, suggesting its involvement in the movement of Se into the leaf tissue ( Wang et al., 2021 ).…”

“…ABC transporters may also be involved in Se absorption and transport in plants. ABCC11, ABCC13, and ABCC10 are implicated in the accumulation and transportation of nanoselenium in cowpeas, regulating Se absorption and transformation ( Li et al., 2023 ). Studies indicate that Se may regulate the expression levels of GSTs, affecting transcription factor activity or participating in signal transduction pathways ( Zheng et al., 2023 ).…”

Comparative transcriptomics provides novel insights into the mechanisms of selenium accumulation and transportation in tea cultivars (Camellia sinensis (L.) O. Kuntze)

“…In rice, ABC transporters were detected under the Na 2 SeO 3 treatment, indicating that they might participate in Se accumulation and transportation ( Kong et al., 2021 ). Meanwhile, the ABCB, ABCC, and ABCG transporters have been suggested to be involved in the accumulation and transport of Se in cowpeas ( Li et al., 2023 ). Fourteen upregulated ABC genes were also found in our study ( Figure 4 ), including ABCA , ABCB , ABCC , and ABCG , indicating that they may play vital roles in the accumulation and transportation of Se in tea roots.…”

Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions

“…In this study, CNGC1 is a cyclic nucleotide-gated ion channel, and its high expression mediates the physiological and biochemical response of dandelion in the transport of Se to plant root cells under Na 2 SeO 3 treatment (Figure 12). In contrast to previous studies [12,34,48,50,52], the identification of these genes in dandelions treated with selenium offers valuable insights into the molecular mechanisms underlying selenium absorption and transport in crops, thus providing new supplements to the existing knowledge in this field.…”

“…ABC transporters are a class of transmembrane transporters that utilize the energy generated by ATP hydrolysis to extensively transfer various chemical substrates [51]. Li's research indicated that ABC transporters play a positive role in Se absorption response, with ABCB, ABCC, and ABCG subfamilies playing important roles in Se absorption and transport in cowpeas [52]. Chen's research found that after spraying Na 2 SeO 3 on the leaves of the B. papyrifera, the expression levels of ABC transporters such as ABC11 and ABC5 were significantly upregulated, indicating that these genes may be involved in the transport of Na 2 SeO 3 to vacuoles [53].…”