Effect of naloxone-precipitated morphine withdrawal on CRH and vasopressin mRNA expression in the rat hypothalamic paraventricular nucleus

Milanés, M. Victoria; Laorden, M. Luisa; Angel, E.; Tankosic, P.; Burlet, Arlette

doi:10.1016/s0304-3940(02)01036-4

Cited by 14 publications

(17 citation statements)

References 15 publications

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“…These results are in line with earlier findings of HPA axis activation by opiate withdrawal, after tolerance has developed following chronic morphine exposure (Ignar and Kuhn, 1990;Zhou et al, 2006). As reported before, a blunting of HPA axis activity in response to chronic opiates is associated with an opiate-induced reduction of CRF gene expression in the hypothalamus (Milanes et al, 2002). Chronic intermittent exposure to opiates is a chronic stressor (Houshyar et al, 2003), and it may modulate the responsivity of the HPA axis at the corticotrope level, as do many other stressors (Shiomi et al, 1986;Zhou et al, 2003).…”

Section: Discussionsupporting

confidence: 92%

Involvement of Arginine Vasopressin and V1b Receptor in Heroin Withdrawal and Heroin Seeking Precipitated by Stress and by Heroin

Zhou

Leri

Cummins

et al. 2007

Neuropsychopharmacol

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A previous study has shown that the stress responsive neurohormone arginine vasopressin (AVP) is activated in the amygdala during early withdrawal from cocaine. The present studies were undertaken to determine whether (1) AVP mRNA levels in the amygdala or hypothalamus, as well as hypothalamic-pituitary-adrenal (HPA) activity, would be altered during chronic intermittent escalating heroin administration (10 days; 7.5-60 mg/kg/day) or during early (12 h) and late (10 days) spontaneous withdrawal; (2) foot shock stress would alter AVP mRNA levels in the amygdala or hypothalamus in rats withdrawn from heroin self-administration (7 days, 3 h/day, 0.05 mg/kg/ infusion); and (3) the selective V1b receptor antagonist SSR149415 (1 and 30 mg/kg, intraperitoneal) would alter heroin seeking during tests of reinstatement induced by foot shock stress and by heroin primes (0.25 mg/kg), as well as HPA hormonal responses to foot shock. We found that AVP mRNA levels were increased during early spontaneous withdrawal in the amygdala only. This amygdalar AVP mRNA increase was no longer observed at the later stage of heroin withdrawal. Foot shock stress increased AVP mRNA levels in the amygdala of rats withdrawn from heroin self-administration, but not in heroin naïve rats. Behaviorally, SSR149415 dose-dependently attenuated foot shock-induced reinstatement and blocked heroin-induced reinstatement. Finally, SSR149415 blunted the HPA activation by foot shock. Together, these data in rats suggest that stress responsive AVP/V1b receptor systems (including the amygdala) may be critical components of the neural circuitry underlying the aversive emotional consequences of drug withdrawal, as well as the effect of negative emotional states on drug-seeking behavior.

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Section: Discussionsupporting

confidence: 92%

Involvement of Arginine Vasopressin and V1b Receptor in Heroin Withdrawal and Heroin Seeking Precipitated by Stress and by Heroin

Zhou

Leri

Cummins

et al. 2007

Neuropsychopharmacol

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“…(3) Following spontaneous withdrawal for 12 h from this chronic morphine administration, there was a significant elevation of plasma ACTH levels. These results confirm earlier findings of HPA axis activation by morphine withdrawal after tolerance has developed following chronic morphine exposure (Buckingham & Cooper 1984, Ignar & Kuhn 1990, Martinez et al 1990, Milanes et al 2002). An elevation of corticosterone levels by morphine withdrawal has been reported (Lightman & Young 1988, Martinez et al 1990), but this is observed after immediate (15 min to 4 h) morphine withdrawal precipitated by naloxone.…”

Section: Stress Responses In Acute Morphine Withdrawalsupporting

confidence: 91%

Mu opioid receptor and orexin/hypocretin mRNA levels in the lateral hypothalamus and striatum are enhanced by morphine withdrawal

Zhou¹,

Bendor²,

Hofmann³

et al. 2006

Journal of Endocrinology

109

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In this study, we investigated the effects of acute morphine administration, chronic intermittent escalating-dose morphine administration and spontaneous withdrawal from chronic morphine on mRNA levels of mu opioid receptor (MOP-r), and the opioid peptides pro-opiomelanocortin (POMC) and preprodynorphin (ppDyn) in several key brain regions of the rat, associated with drug reward and motivated behaviors: lateral hypothalamus (lat.hyp), nucleus accumbens (NAc) core, amygdala, and caudate-putamen (CPu). There was no effect on MOP-r mRNA levels in these brain regions 30 min after either a single injection of morphine (10 mg/kg, i.p.) or chronic intermittent escalating-dose morphine (from 7$5 mg/kg per day on day 1 up to 120 mg/kg per day on day 10). Activation of the stress-responsive hypothalamic-pituitary-adrenal axis by 12 h withdrawal from chronic morphine was confirmed; both POMC mRNA levels in the anterior pituitary and plasma adrenocorticotropic hormone levels were significantly elevated. Under this withdrawal-related stress condition, there was an increase in MOP-r mRNA levels in the lat.hyp, NAc core, and CPu. Recent studies have demonstrated a novel role for the lat.hyp orexin (or hypocretin) activation in both drug-related positive rewarding, and withdrawal effects. Around 50% of lat.hyp orexin neurons express MOP-r. Therefore, we also examined the levels of lat.hyp orexin mRNA, and found them increased in morphine withdrawal, whereas there was no change in levels of the lat.hyp ppDyn mRNA, a gene coexpressed with the lat.hyp orexin. Our results show that there is an increase in MOP-r gene expression in a region-specific manner during morphine withdrawal, and support the hypothesis that increased lat.hyp orexin activity plays a role in morphine-withdrawalrelated behaviors.

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“…Maj et al (15) demonstrated that CRH mRNA was increased in the hypothalamus after acute and chronic administration of morphine. However, another study (16) found a decrease of CRH mRNA in the hypothalamus potentially resulting from negative feedback on the HPA axis (17) during which expression of CRH mRNA was suppressed by increasing COR. This study was consistent with previous reports demonstrating that chronic morphine treatment and withdrawal decreases CRH mRNA (16).…”

Section: Discussionmentioning

confidence: 99%

“…However, another study (16) found a decrease of CRH mRNA in the hypothalamus potentially resulting from negative feedback on the HPA axis (17) during which expression of CRH mRNA was suppressed by increasing COR. This study was consistent with previous reports demonstrating that chronic morphine treatment and withdrawal decreases CRH mRNA (16). However, the Milanés et al study (16) examined Sprague-Dawley rats.…”

Section: Discussionmentioning

confidence: 99%

Dysfunction of the Hypothalamic–Pituitary–Adrenal Axis in Opioid Dependent Subjects: Effects of Acute and Protracted Abstinence

Zhang

Ren

Sheng

et al. 2008

The American Journal of Drug and Alcohol Abuse

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Effect of naloxone-precipitated morphine withdrawal on CRH and vasopressin mRNA expression in the rat hypothalamic paraventricular nucleus

Cited by 14 publications

References 15 publications

Involvement of Arginine Vasopressin and V1b Receptor in Heroin Withdrawal and Heroin Seeking Precipitated by Stress and by Heroin

Involvement of Arginine Vasopressin and V1b Receptor in Heroin Withdrawal and Heroin Seeking Precipitated by Stress and by Heroin

Mu opioid receptor and orexin/hypocretin mRNA levels in the lateral hypothalamus and striatum are enhanced by morphine withdrawal

Dysfunction of the Hypothalamic–Pituitary–Adrenal Axis in Opioid Dependent Subjects: Effects of Acute and Protracted Abstinence

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