Effect of NaCl stress on physiological, antioxidant enzymes and anatomical responses of Astragalus gombiformis

Boughalleb, Fayçal; Abdellaoui, Raoudha; Nbiba, Nader; Mahmoudi, Maher; Neffati, Mohamed

doi:10.1515/biolog-2017-0169

Cited by 13 publications

(14 citation statements)

References 89 publications

(97 reference statements)

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“…Similarly, Gil-Quintana et al (2013) showed that sucrose, glucose, and fructose are the most common soluble sugars accumulated in plants subjected to abiotic stress. The accumulation of sucrose and glucose compared to the proline content suggests their main role in the process of osmotic adjustment in P. equisetiforme under salt stress, which is in agreement with results on Astragalus gombiformis (Boughalleb et al, 2017) and Stipa lagascae (Abdellaoui et al, 2017). In view of the positive and highly significant correlation between MDA and the accumulation of soluble sugars (i.e., fructose, glucose, and sucrose) (Table 3), they could act as scavengers of free radicals and provide membrane protection, which is similar to the results of Singh et al (2015).…”

Section: Discussionsupporting

confidence: 91%

“…In addition to the vital role of metabolites in scavenging ROS, several antioxidant enzymes are associated with the antioxidant defense system which helps plants to better overcome abiotic stresses (Apel and Hirt, 2004). In our study, SOD activity increased at higher salinity levels (300-400 mM NaCl), while it remained constant at low and moderate doses of NaCl (≤200 mM), similar to results obtained in Astragalus gombiformis (Boughalleb et al, 2017) and Stipa lagascae (Abdellaoui et al, 2017). This increase in SOD activity generates high levels of H 2 O 2 , which acts as a precursor for other ROS (Bose et al, 2014).…”

Section: Nosupporting

confidence: 88%

“…Furthermore, the Ψ W of P. equisetiforme was significantly decreased with increasing salinity. In the cell cytoplasm, reduced water potential can be interpreted as an adaptive mechanism to allow the cells which are subjected to lower water to have the potential to improve their ability to maintain turgor pressure (Boughalleb et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Salinity is known as the most significant serious environmental issue affecting plant production and growth, particularly in arid regions. It usually stimulates declines in stomatal conductance and changes in the photochemical apparatus, which may eventually decrease photosynthetic rates (Boughalleb et al, 2017;Abdellaoui et al, 2017). The inhibitory consequences of salinity also contribute to alterations in metabolic perturbation, water relations, reactive oxygen species (ROS) production, ion toxicity, and tissue damage (Zhu, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the role of secondary metabolites (nonenzymatic antioxidants) in plant protection against oxidative stress, other enzymatic defense systems such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and glutathione reductase (GR) are implicated (Parida and Jha, 2010). Antioxidant enzyme activities in various plant species are correlated with their tolerance to salinity (Abdellaoui et al 2017;Boughalleb et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Changes in phenolic profile, soluble sugar, proline, and antioxidant enzyme activities of Polygonumequisetiforme in response to salinity

Boughalleb¹,

Abdellaoui²,

Mahmoudi³

et al. 2020

Turk J Bot

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This study intended to focus on the effect of different levels of NaCl (0-400 mM, at regular intervals every 100 mM) on the growth, secondary metabolites, and antioxidant enzyme activities of Polygonum equisetiforme, a wild plant with medicinal and industrial uses, which often grows in harsh environmental conditions. Our results showed that biomass production significantly decreased with salinity, while the leaf relative water content declined only at high levels of NaCl concentration (300-400 mM). In contrast, these higher doses of salt resulted in a significant increase in malondialdehyde (MDA) content. Both proline and soluble sugar (e.g., fructose, glucose, and sucrose) contents were enhanced under saline conditions. The methanolic extracts of the shoots included 10 flavonoids and 9 phenolic acids. The total phenolic acids (TPA), total flavonoid compounds (TFC), and total phenolic compounds (TPC) increased with salinity, particularly at 300 mM NaCl. An increase in TPA resulted especially from an increase in quinic, gallic, and protocatechuic acids (phenolic compounds), followed by quercetin-3-O-galactoside, catechin, and epicatechin (flavonoid compounds). Superoxide dismutase (SOD) activity increased only at high salinity levels (>200 mM), while glutathione reductase (GR), guaiacol peroxidase (GPX), catalase (CAT), and ascorbate peroxidase (APX) activity increased with salinity level. A positive significant correlation between antioxidant DPPH and TPA, TFC, TPC, CAT, and APX suggests a vital protective role in controlling oxidative stress through the scavenging process. Consequently, our results indicated that P. equisetiforme shoots are rich in secondary metabolites, especially phenolic compounds with high potential antioxidant activities. It can be considered a salt-tolerant species able to survive at salinity levels up to 300 mM NaCl.

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