Effect of NaCl on ionic content and distribution in suspension-cultured cells of the halophyte Sonneratia alba versus the glycophyte Oryza sativa

Hayatsu, Manabu; Suzuki, Suechika; Hasegawa, Akira; Tsuchiya, Shinpei; Sasamoto, Hamako

doi:10.1016/j.jplph.2014.06.008

Cited by 13 publications

(22 citation statements)

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“…The fixed specimens were dehydrated with a graded acetone series and embedded in Quetol 812 epoxy resin (Nisshin EM Co. Ltd., Tokyo, Japan). The preparation of ultrathin sections, electron staining with uranyl acetate and lead citrate, and microscope observation were carried out as described previously (Hayatsu et al, 2012(Hayatsu et al, , 2014. X-ray microanalysis was performed as detailed in section 2.6 on the electron-dense precipitates at the middle lamella of cell wall in the resin-embedded sections without electron staining as described previously (Inoue et al, 2013).…”

Section: Conventional Transmission Electron Microscopy and X-ray Micrmentioning

confidence: 99%

“…The method used for the cryosection preparation, freeze-drying, and the quantitative X-ray microanalysis was essentially the same as in our earlier studies (Hayatsu et al, 2012(Hayatsu et al, , 2014. Briefly, samples in agarose blocks were high-pressure frozen (EM-PACT, Leica, Austria) and stored in liquid N 2 .…”

Section: Preparation Of Cryosectionsmentioning

confidence: 99%

“…Quantitative X-ray microanalysis of cryosections was performed as described previously (Hayatsu et al, 2012(Hayatsu et al, , 2014 using an analytical electron microscope (JEM 1230/MiniCup/EX-14033JTP: JEOL, Akishima, Tokyo, Japan) with a Be-stage holder or a cryotransfer holder (G626DH: Gatan, Tokyo, Japan), respectively. Elemental concentrations were calculated on the basis of the Peak/Background intensity ratio (P/B ratio) using the software NORAN System SIX (Thermo Electron Co., Middleton, WI, USA) and the concentrations were indicated in mmol /kg dry weight (DW).…”

Section: X-ray Microanalysismentioning

confidence: 99%

“…Previously, distribution of cellular elements, in cryosections of S. alba suspension cells was compared with that of glycophyte rice suspension cells (Hayatsu, Suzuki, Hasegawa, Tsuchiya, & Sasamoto, 2014). We found that decrease of cellular Ca concentration ([Ca]) in the cytoplasmic matrix and vacuole was related to the halophilic nature of S. alba to Na + .…”

Section: Introductionmentioning

confidence: 98%

“…Furthermore, a 'low-Ca callus line' was established by sub-culturing in the medium without Ca 2+ . To determine the cellular mechanisms underlying Ca 2+ inhibition in callus culture of A. alba, we studied the ultrastructural features of cells, and intra-(cytoplasmic matrix and vacuole) and extracellular (cell wall) [Ca] and concentrations of various elements, using electron probe X-ray microanalysis and compared the results with those of S. alba (Hayatsu, Ono, Hamamoto, & Suzuki, 2012;Hayatsu et al, 2014). Effects of deletion of CaCl 2 in the medium were also investigated on growth for their protoplast cultures.…”

Section: Introductionmentioning

confidence: 99%

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Cellular Calcium Distribution Modulates the Growth of Callus and Protoplasts of Halophyte Mangrove Plant, Avicennia Alba - an X-ray Microanalysis

Hayatsu¹,

Suzuki²,

Tsuchiya³

et al. 2017

JPS

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Two cultured cell lines were developed from cotyledons of a halophyte mangrove, Avicennia alba. In the high-Ca callus line, which was sub-cultured in a modified amino acid medium containing 3 mM CaCl 2 , growth of calluses and their protoplasts were both inhibited by low concentrations of CaCl 2 in the culture medium. Removal of Ca 2+ from the culture medium stimulated callus growth and the calluses could be sub-cultured without CaCl 2 (low-Ca callus line). The intra-(cytoplasmic matrix and vacuole) and extra-(cell wall) cellular concentrations of elements, i.e., [Ca] [S] were investigated using quantitative X-ray microanalysis of cryosections of calluses from both cell lines. [Ca] was high in the cytoplasmic matrix and cell wall of the high-Ca line. [Ca] was lowered in the low-Ca line in all cell compartments, though still detected. Ca-containing electron-dense precipitates were accumulated in the middle lamella of cell walls in resin-embedded sections of the high-Ca line. CaCl 2 in the medium stimulated protoplast growth only in the low-Ca line. These results suggested that a low cellular [Ca] is needed for protoplasts growth of A. alba. The importance of cellular [Ca] for the growth of halophilic mangrove plant cells was discussed.

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Section: Conventional Transmission Electron Microscopy and X-ray Micrmentioning

confidence: 99%

Section: Preparation Of Cryosectionsmentioning

confidence: 99%

Section: X-ray Microanalysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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