Effect of NaCl addition on the production of welan gum with the UV mutant of Sphingomonas sp

Ke, Chengzhu; Wei, Lulu; Miao, Wang; Li, Qiwen; Liu, Xiaoling; Guo, Yuan; Li, Shubo

doi:10.1016/j.carbpol.2021.118110

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…Six BGCs regions were identified from the whole genome of AK-PDB1-5 T (Supplementary Table S2 and Supplementary Figures S8-S13), which contained sphingan polysaccharide, malleobactin A/malleobactin B/ malleobactin C/malleobactin D and zeaxanthin. Sphingan polysaccharide is a very common compound in this genus, exemplified by compounds such as WL gum, gallen gum, rhamsan gum, and welan gum (Zheng et al, 2018;Baez et al, 2019;Li et al, 2019;Zhu et al, 2019Zhu et al, 2020Zhu et al, , 2021Huang et al, 2020;Liu et al, 2020;Xu et al, 2020;Ke et al, 2021;Zhao et al, 2021). The sphingans group has applications in a variety of industries as food, cement, personal care, abiotic stress tolerance, bioremediation, and biodegradation, etc.…”

Section: Metabolite Biosynthesis Gene Clusters Analysismentioning

confidence: 99%

A nostoxanthin-producing bacterium, Sphingomonas nostoxanthinifaciens sp. nov., alleviates the salt stress of Arabidopsis seedlings by scavenging of reactive oxygen species

et al. 2023

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A novel, nostoxanthin-producing, endophytic bacterium, designated as AK-PDB1-5T, was isolated from the needle-like leaves of the Korean fir (Abies koreana Wilson) collected from Mt. Halla in Jeju, South Korea. A 16S rRNA sequence comparison indicated that the closest phylogenetic neighbors were Sphingomonas crusticola MIMD3T (95.6%) and Sphingomonas jatrophae S5-249T (95.3%) of the family Sphingomonadaceae. Strain AK-PDB1-5T had a genome size of 4,298,284 bp with a 67.8% G + C content, and digital DNA–DNA hybridization and OrthoANI values with the most closely related species of only 19.5–21% and 75.1–76.8%, respectively. Cells of the strain AK-PDB1-5T were Gram-negative, short rods, oxidase- and catalase-positive. Growth occurred at pH 5.0–9.0 (optimum pH 8.0) in the absence of NaCl at 4–37°C (optimum 25–30°C). Strain AK-PDB1-5T contained C14:0 2OH, C16:0 and summed feature 8 as the major cellular fatty acids (> 10%), while sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, phospholipids and lipids were found to be the major polar lipids. The strain produces a yellow carotenoid pigment; natural products prediction via AntiSMASH tool found zeaxanthin biosynthesis clusters in the entire genome. Biophysical characterization by ultraviolet–visible absorption spectroscopy and ESI-MS studies confirmed the yellow pigment was nostoxanthin. In addition, strain AK-PDB1-5T was found significantly promote Arabidopsis seedling growth under salt conditions by reducing reactive oxygen species (ROS). Based on the polyphasic taxonomic analysis results, strain AK-PDB1-5T was determined to be a novel species in the genus Sphingomonas with the proposed name Sphingomonas nostoxanthinifaciens sp. nov. The type strain is AK-PDB1-5T (= KCTC 82822T = CCTCC AB 2021150T).

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Section: Metabolite Biosynthesis Gene Clusters Analysismentioning

confidence: 99%

A nostoxanthin-producing bacterium, Sphingomonas nostoxanthinifaciens sp. nov., alleviates the salt stress of Arabidopsis seedlings by scavenging of reactive oxygen species

et al. 2023

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“…Weilan gum (WLG) polysaccharide is a macromolecular exopolysaccharide secreted by Alcaligenes ( 13 ) that has broad commercial applications in the food industry as a thickener, suspending agent, binder and emulsifier ( 14 ). For example, WLG functions as a protective colloid and plays an important role in eliminating protein flocculation in dairy products ( 15 , 16 ).…”

Section: Introductionmentioning

confidence: 99%

Weilan gum oligosaccharide ameliorates dextran sulfate sodium‑induced experimental ulcerative colitis

Zhang

et al. 2021

Mol Med Rep

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ulcerative colitis (uc) is a global disease, characterized by periods of relapse that seriously affects the quality of life of patients. oligosaccharides are considered to be a prospective strategy to alleviate the symptoms of uc. The present study aimed to evaluate the effect of weilan gum oligosaccharide (WlGo) on a mouse uc model induced by dextran sulfate sodium (dSS). WlGo structural physical properties were characterized by electrospray mass spectrometry and fourier tansform infrared spectroscopy. MTT assays were performed to evaluate the non-toxic concentration of WlGo. rT-qPcr and eliSas were conducted to determine the levels of inflammatory factors. The clinical symptoms and mucosal integrity of the dSS-induced uc model were assessed by dai and histological assessment. lPS-induced caco-2 cells and dSS-induced uc mice were used to explore the effects of WlGo on uc. Treatment of the mice with 4.48 g/kg/day WLGO via gavage for 7 days significantly relieved the symptoms of DSS-induced UC model mice, whereas significant effects were not observed for all symptoms of dSS-induced uc in the WlGo-low group. The disease activity index score was decreased and the loss of body weight was reduced in dSS-induced uc model mice treated with WlGo. Moreover, colonic damage and abnormally short colon length shortenings were relieved following WlGo treatment. WlGo treatment also reduced the concentration and mrna expression levels of proinflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor α, in dSS-induced uc model mice and lipopolysaccharide-treated caco-2 cells. These results indicated that WlGo may be an effective strategy for uc treatment.

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Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01

Liu,

He,

Wei

et al. 2024

J Sci Food Agric

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BACKGROUNDAs a novel type of extracellular polysaccharide produced by Sphingomonas sp., welan gum has been widely applied in various fields because of its excellent properties. The study has improved the fermentation process.ResultsThe initial sucrose concentration, temperature and stirring speed were set to 20 g L−1, 33 °C and 400 rpm, respectively, and 13.3 g L−1 sucrose was added at 24, 40 and 56 h. The temperature and stirring speed were then set at 28 °C and 600 rpm from 24 to 48 h and 28 °C and 600 rpm from 48 to 72 h, respectively. As a result, welan gum production, dry cell weight, sucrose conversion rate and viscosity were correspondingly increased to 38.60 g L−1, 5.47 g L−1, 0.64 g g−1 and 3779 mPa·s, respectively. In addition, the mechanism by which fermentation strategy promotes welan gum synthesis was investigated by transcriptome analysis.CONCLUSIONImproving respiration and ATP supply, reducing unnecessary protein synthesis, and alleviating competition between cell growth and welan gum synthesis contribute to promoting the fermentation performance of Sphingomonas sp., thus providing a practical strategy for efficient welan gum production. © 2024 Society of Chemical Industry.

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Effect of NaCl addition on the production of welan gum with the UV mutant of Sphingomonas sp

Cited by 6 publications

References 25 publications

A nostoxanthin-producing bacterium, Sphingomonas nostoxanthinifaciens sp. nov., alleviates the salt stress of Arabidopsis seedlings by scavenging of reactive oxygen species

A nostoxanthin-producing bacterium, Sphingomonas nostoxanthinifaciens sp. nov., alleviates the salt stress of Arabidopsis seedlings by scavenging of reactive oxygen species

Weilan gum oligosaccharide ameliorates dextran sulfate sodium‑induced experimental ulcerative colitis

Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01

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