Effect of n‐Butyl‐2‐cyanoacrylate on the Tensile Strength and Pressure Resistance of Tracheal Anastomoses Ex Vivo

Biçer, Yusuf; Koybasi, Serap; Kazaz, Hasan; Seyhan, Sinan

doi:10.1177/0194599814560140

Cited by 4 publications

(8 citation statements)

References 28 publications

(61 reference statements)

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“…4,5,15) Bicer et al, in sheep, they found the rupture tension as 171.9 Newtons. 16) In this study, the GM of our cases was 17032.2 grams-force (167.03 Newtons), 14594.8 grams-force (143.13 Newtons), and 12852.1 grams-force (126.04 Newtons) for Groups 1, 2, and 3, respectively. From this perspective, our study data are consistent with the literature.…”

Section: Discussionmentioning

confidence: 44%

Despite the Effects of Tension and Intraluminal Pressure, Which Suture Technique Is the Most Appropriate for Prevention of Air Leakage or Anastomotic Dehiscence in Tracheal Anastomoses in the Short Term? An Experimental Research on Ex Vivo Model

Ersöz

2019

ATCS

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Purpose: We performed an experimental study comparing different suture techniques in trachea anastomoses using the ex vivo sheep model, which deals with the parameters that suture tension, air leakage, intraluminal pressure, and tension at which the anastomosis will rupture. We aimed to find an answer to “Which suture technique should be used in tracheal anastomoses?”Methods: In all, 45 sheep tracheas were randomly divided into three groups (each n = 15) differing in suture technique for anastomoses: single stitches, mixed, and continuous suture. The anastomoses were evaluated for air leakage under normal (25 mbar) and high (70 mbar) intraluminal pressures without tension. Then, air leakage was followed under high intraluminal pressure with tensile stress. Tension levels of dehiscence were also recorded. Data were statistically evaluated.Results: No air leakage was observed at 25 mbar intraluminal pressure. At 70 mbar pressure without tension, no statistically significant difference was found among the groups (p >0.05). However, single-stitch technique was the best in terms of air leakage tension and rupture tension levels (p <0.05).Conclusion: The most reliable and advantageous is single-stitch technique for a tracheal anastomosis in short-term results. Further studies are needed to analyze longer ventilation periods in terms of other serious complications as ischemic dehiscence and stenosis.

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Section: Discussionmentioning

confidence: 44%

Despite the Effects of Tension and Intraluminal Pressure, Which Suture Technique Is the Most Appropriate for Prevention of Air Leakage or Anastomotic Dehiscence in Tracheal Anastomoses in the Short Term? An Experimental Research on Ex Vivo Model

Ersöz

2019

ATCS

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“…Many studies have described the use of NBCA as a tissue adhesive. NBCA has been used with variable success rates for the treatment of bone fractures, ossicular fixation, embolization, fistulas, and hemostasis of esophageal varices or bleeding gastric ulcers as well as for vascular or tracheal anastomoses [71316171819202122232425]. These remarkable studies attempted to show the potential applications of NBCA.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of n-butyl-2-cyanoacrylate (Histoacryl) in cervical structures of rats: prospective in vivo study

Suh

Kim

et al. 2019

Ann Surg Treat Res

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Purpose We investigated the biocompatibility of n-butyl-2-cyanoacrylate (NBCA) in the cervical deep tissues of rats to assess its biocompatibility. Methods A total of 30 Sprague-Dawley rats were injected with NBCA. After 30, 90, 180, and 360 days, cubes of tissue (1 cm × 1 cm × 1 cm) surrounding the NBCA and normal tissue from the other side of the neck were excised from each rat. The adhesion of NBCA to adjacent structures was examined histologically. Cells were counted per high-power field (HPF), and fibrosis was graded with the measurement of fibrotic thickening. Results All animals displayed normal behavior without any symptoms of distress throughout the study. There was no recognizable inflammatory reaction, foreign body reaction, or fibrosis in the 30 control samples. The analyses of experimental samples showed significantly decreased inflammatory cell counts over time (lymphoplasma cell count decreased from 100 (range, 70–100) to 30 (range, 30–50) per HPF, P = 0.010; neutrophil count decreased from 2 (range, 2–30) to 0 (range, 0–2) per HPF, P = 0.017). However, there was no significant difference in the number of multinuclear giant cells throughout the study period (a decrease from 22 [range, 16–34] to 16 [range, 12–22] per HPF, P = 0.287). The level of fibrosis was Common Toxicity Criteria ver. 4.0 Grade 1 without further thickening (P = 0.600). However, maturation of fibrosis progressed gradually. Conclusion NBCA was biologically tolerable in the cervical deep tissues of rats. However, precautions are needed with respect to preventing a sustained foreign body reaction and fibrosis.

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“…In a previous ex-vivo study, it was reported that the application of n-butyl-2-cyanoacrylate to the site of the anastomosis in the trachea had beneficial effects in terms of intraluminal pressure. However, another study on rats revealed this application's inflammatory effects, which may undermine its usage in vivo [5,22].…”

Section: Discussionmentioning

confidence: 99%

“…Groups 1 and 3 were subjected to tensile strength experiments and Groups 2 and 4 were subjected to pressure experiments. The tensile strength experiment was performed by using an electronic spring scale, with the force needed to break anastomosis being recorded [5] (Figure 2). For the pressure experiment, a sphygmomanometer was attached to a cuffed 8.5 mm endotracheal tube, with the pressure at air leakage observed being recorded [5] ( Figure 3).…”

Section: Methodsmentioning

confidence: 99%

“…The tensile strength experiment was performed by using an electronic spring scale, with the force needed to break anastomosis being recorded [5] (Figure 2). For the pressure experiment, a sphygmomanometer was attached to a cuffed 8.5 mm endotracheal tube, with the pressure at air leakage observed being recorded [5] ( Figure 3). SPSS for Windows (version17.0, IBM, Armonk, New York) was used for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

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The effect of polyglycolic acid mesh application in tracheal anastomoses on pressure and tensile strength: an ex vivo experimental study

Kılıçgün¹,

Biçer²,

Kutluk³

et al. 2019

Curr Thorac Surg

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To cite this article: Kılıçgün A, Biçer YO, Kutluk AC, Seyhan S, Köybaşı Sanal S. The effect of polyglycolic acid mesh application in tracheal anastomoses on pressure and tensile strength: an ex vivo experimental study. Curr ABSTRACTBackground: This study aims to investigate the effect of polyglycolic acid (PGA) mesh application in tracheal anastomosis on the air-leakage and tension resistance. Materials and Methods:Tracheae were collected from freshly slaughtered healthy sheep weighing 25-30 kg (2-3 years old) that were acquired from the local slaughterhouse. Twenty-eight tracheae were transported to the laboratory within an hour of slaughter and then dissected to remove the esophagus before obtaining 20-cm long tracheae. All tracheae were transected at the midpoint. In Groups 1 and 2, the transected tracheae were anastomosed by using interrupted 3-0 Vicryl sutures. In Groups 3 and 4, after anastomosing the transected tracheae with interrupted 3-0 Vicryl, the sites of anastomosis were reinforced by using Polyglycolic acid mesh. Groups 1 and 3 were tested for tensile strength, while Groups 2 and 4 were tested for pressure resistance. Results:The median pressure was 49.00 ± 7.47 mmHg in the group anastomosed with only Vicryl, whereas it was measured as 70 ± 13.49 mmHg in the group on which the anastomoses were reinforced with PGA mesh. The median force needed to break anastomoses were 166.40 ± 24.28 Newton in the Vicryl-only group and 136.80 ± 34.93 newton in the PGA-reinforced group. The difference between the pressure resistance values of the Vicryl-only group and the PGA reinforced group was significant (p = 0.006). However, the difference with regard to the tensile strength was insignificant (p = 0.201). Conclusion:We found that the reinforcement of the anastomosis site with polyglycolic acid mesh significantly increases the pressure resistance but does not significantly affect tensile strength.

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Effect of n‐Butyl‐2‐cyanoacrylate on the Tensile Strength and Pressure Resistance of Tracheal Anastomoses Ex Vivo

Cited by 4 publications

References 28 publications

Despite the Effects of Tension and Intraluminal Pressure, Which Suture Technique Is the Most Appropriate for Prevention of Air Leakage or Anastomotic Dehiscence in Tracheal Anastomoses in the Short Term? An Experimental Research on Ex Vivo Model

Despite the Effects of Tension and Intraluminal Pressure, Which Suture Technique Is the Most Appropriate for Prevention of Air Leakage or Anastomotic Dehiscence in Tracheal Anastomoses in the Short Term? An Experimental Research on Ex Vivo Model

Biocompatibility of n-butyl-2-cyanoacrylate (Histoacryl) in cervical structures of rats: prospective in vivo study

The effect of polyglycolic acid mesh application in tracheal anastomoses on pressure and tensile strength: an ex vivo experimental study

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