Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia-reperfusion: histopathological study in rat model

Sotoudeh, Ahmad; Takhtfooladi, Mohammad Ashrafzadeh; Jahanshahi, Amirali; Asl, Adel Haghighi Khiabanian; Takhtfooladi, Hamed Ashrafzadeh; Khansari, Mohammadreza

doi:10.1590/s0102-86502012000200012

Cited by 35 publications

(29 citation statements)

References 18 publications

(9 reference statements)

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“…Major reasons of acute extremity ischemia are thrombosis and embolism 3 . Additionally ischemia reperfusion (IR) can be caused by numerous events such as trauma, various orthopaedic surgery and freeflap reconstruction 4,5 . A huge number of consecutive events are initiated by free oxygen radicals.…”

Section: Introductionmentioning

confidence: 99%

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Akdemir

Gülçın

Karagöz

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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In this study, we investigated the potential beneficial effects of quercetin on skeletal muscle ischemia reperfusion injury. Twenty-four Sprague-Dawley type rats were randomly divided into four groups. In the sham group, only gastrocnemius muscle were removed and given no quercetin. In ischemia group, all the femoral artery, vein and collaterals were occluded in the left hindlimb by applying tourniquate under general anaesthesia for three hours but reperfusion was not done. In the Quercetin + Ischemia reperfusion group, quercetin (200 mg kg À1 dose orally) was given during one-week reoperation and later ischemia reperfusion model was done. Finally, gastrocnemius muscle samples were removed to measure biochemical parameters. The biomarkers, MDA levels, SOD, CAT and GPx activities, were evaluated related to skeletal muscle ischemia reperfusion injury. MDA levels reduced and SOD, CAT and GPx activities increased significantly in Quercetin + Ischemia reperfusion group. Results clearly showed that Quercetin have a protective role against oxidative damage induced by ischemia reperfusion in rats.

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Section: Introductionmentioning

confidence: 99%

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Akdemir

Gülçın

Karagöz

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Metabolites of erdosteine have two sulfhydryl groups and they take part in free radical scavenging and antioxidant activity (13). Free radical scavenging properties of erdosteine have been shown in experimental and clinical studies (1,6,7,11).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of erdosteine on erythrocyte deformabilityin a rat model of lower limb ischemia/reperfusion injury

Özer¹,

Demirtaş²,

Çomu³

et al. 2018

Turk J Med Sci

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Background/aim: The protective effect of erdosteine on local and distant organ injury due to ischemia/reperfusion has been well documented but its effect on erythrocyte deformability needs further investigation. Our aim was to investigate the effect of erdosteine on erythrocyte deformability in the infrarenal aorta of rats undergoing ischemia/reperfusion. Materials and methods:Our study was conducted with 18 Wistar albino rats. Rats were divided into 3 groups: a randomized control group (group 'control' , n = 6), an ischemia/reperfusion group without erdosteine (group 'ischemia/reperfusion' , n = 6), and an ischemia/ reperfusion group with erdosteine at 150 mg kg -1 , intraperitoneally (group 'ischemia/reperfusion -erdosteine' , n = 6). Packs of erythrocytes were prepared from heparinized blood samples and deformability measurements were conducted.Results: Comparisons of the control and ischemia/reperfusion -erdosteine groups revealed similar results (P = 0.051). The values of the ischemia/reperfusion group were significantly higher than those of the control and ischemia/reperfusion -erdosteine groups (P < 0.0001 and P = 0.024, respectively). Relative resistance, a marker of erythrocyte deformability, was increased significantly by ischemia/ reperfusion compared to the control and ischemia/reperfusion -erdosteine groups (P < 0.05). Conclusion:We detected unfavorable effects of ischemia/reperfusion on erythrocyte deformability, which may lead to disturbance in blood flow and hence tissue perfusion in the infrarenal rat aorta. We also found that erdosteine had beneficial effects by reversing undesirable effects of ischemia/reperfusion. However, these promising results should be further supported by more detailed studies with larger volumes.

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“…Temporary aortic cross-clamping is accompanied by I/R injury and a systemic infl ammatory response syndrome that can lead to non-cardiogenic pulmonary oedema, or acute respiratory distress syndrome in abdominal aortic surgery (13,14). In the development of lung injury, the excessive generation of reactive oxygen radicals, polymorphonuclear sequestration in pulmonary microvasculature, increased endothelial permeability and interstitial edema have been suggested (15).…”

Section: Discussionmentioning

confidence: 99%

Antioxidative effects of adrenomedullin and vascular endothelial growth factor on lung injury induced by skeletal muscle ischemia-reperfusion

Gl¹,

Kirişçi²,

Dursun³

et al. 2013

BLL

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Abstract:Purpose: The aim of this study was to investigate the effects of adrenomedullin (AM) and vascular endothelial growth factor (VEGF) on lung injury as a remote organ following skeletal muscle ischemia-reperfusion injury in a rat model. Materials and methods: Thirty-six Wistar rats were randomized into six groups (n=6). Laparotomy was performed in all groups under general anesthesia. Nothing else was done in Group S (Sham). Ischemia reperfusion group (Group I/R) underwent ischemia and reperfusion performed by clamping and declamping of the infrarenal abdominal aorta for 120 minutes, respectively. Group VEGF and Group AM received intravenous infusion of VEGF (0.8 μg/kg) or AM (12 μg /kg) respectively, without ischemia and reperfusion. Group IR+VEGF and Group IR+AM received intravenous infusion of VEGF (0.8 μg/kg) or AM (12 μg /kg) respectively immediately after 2 hours period of ischemia. At the end of reperfusion period. Lung tissue samples were taken for biochemical examination. Total oxidant status (TOS) and total antioxidant status (TAS) levels in lung tissue were determined by using a novel automated method. p<0.05 was considered as statistically signifi cant. Results: TOS levels were signifi cantly higher in Group I/R, when compared with groups S, AM and VEGF (p=0.004, p=0.011, p=0.017, respectively) and signifi cantly lower in groups I/R+AM and I/R+VEGF, when compared with Group I/R (p=0.018, p=0.006, respectively). TAS levels were signifi cantly higher in Group I/R, when compared with groups S, AM and VEGF (p=0.006 p=0.016, p=0.016, respectively) and signifi cantly lower in Group I/R+AM, when compared with Group I/R (p=0.016). Conclusion: These fi ndings indicate that AM and VEGF acted effectively on the prevention of lung injury induced by skeletal muscle ischemia-reperfusion injury in a rat model (Fig. 2, Ref. 30). Full Text in PDF www.elis.sk.

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Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia-reperfusion: histopathological study in rat model

Cited by 35 publications

References 18 publications

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Protective effect of erdosteine on erythrocyte deformabilityin a rat model of lower limb ischemia/reperfusion injury

Antioxidative effects of adrenomedullin and vascular endothelial growth factor on lung injury induced by skeletal muscle ischemia-reperfusion

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