Effect of myo-inositol on the glycerophospholipid composition of adult and fetal rat lung tissue

Quirk, J. Gerald; Baumgarten, Birgit; Bleasdale, John E.

doi:10.1515/jpme.1984.12.4.201

Cited by 11 publications

(5 citation statements)

References 19 publications

(31 reference statements)

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“…Our results also suggest that the PI/PG ratio in the surfactant decrease with the gestationalage-related decrease in fetal rat plasma inositol concentration. The possibility of a causal relationship is supported by the findings of Quirk et al (32), who noticed that elevating the fetal plasma inositol levels in the rat resulted in higher PI/PG ratio of their lung tissue phospholipids. The fetal rat may therefore be a good model for studying factors which affect fetal lung maturation especially with respect to the synthesis of PI and PG.…”

Section: Discussionmentioning

confidence: 89%

Gestational Age-Dependent Changes in Plasma Inositol Levels and Surfactant Composition in the Fetal Rat

Egberts

Noort

1986

Pediatr Res

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ABSTRACT. To study the possibility that changes in fetal surfactant composition depend on the availability of inositol, we isolated surfactant material from lungs of fetal and neonatal rats and estimated their plasma inositol concentration. During the 18-to 22-day gestational period the amount of surfactant increases from 0.17 to 3.10 pmol phospholipids/g wet lung. From day 20 onward, 70% or more of the phospholipids is phosphatidylcholine. In this period the relatively high percentage of phosphatidylinosit01 (8%) in the lung surfactant decreases to 4% whereas the percentage of phosphatidylglycerol increases from 2 to 8% at parturition. During gestation the phospholipid/protein ratio of the surfactant material increase from 3 to 11 and the highest ratio is found immediately after birth. The production of fetal lung surfactant is initiated during the last period of pregnancy. During its maturation changes occur both in the amount produced and in its phospholipid composition (1).In the human fetus these compositional changes include: 1) enrichment in phosphatidylcholine and especially saturated phosphatidylcholine (2, 3), 2) a temporary increase in the percentage of PI and later on a decrease in its percentage with a concomitant increase in the percentage of PG (4).In man, PG is an important minor phospholipid of surfactant and its appearance in amniotic fluid is an indication that the lung will function normally at birth (4).It has been suggested that in the fetus the percentage of PI and PG changes because of a decrease in fetal plasma inositol con-

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Section: Discussionmentioning

confidence: 89%

Gestational Age-Dependent Changes in Plasma Inositol Levels and Surfactant Composition in the Fetal Rat

Egberts

Noort

1986

Pediatr Res

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“…Therefore the influence of myo-inositol on CMP-dependent incorporation of [14C]glycerol 3-phosphate into phosphatidylglycerol by permeabilized type II pneumonocytes was investigated. myo-Inositolwas employed atconcentrations in the range ofthose nmeasured in foetal-rat serum (Burton & Wells, 1974;Quirk et al, 1984). In the absence of added CMP, the addition of myo-inositol (2.0 mM) to permeabilized cells resulted in decreased incorporation of [14C]glycerol 3-phosphate into phosphatidylglycerol and increased incorporation into phosphatidylinositol (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Cytidine monophosphate-dependent synthesis of phosphatidylglycerol in permeabilized type II pneumonocytes

Bleasdale¹,

Thakur²,

Rader³

et al. 1985

Biochemical Journal

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Results of previous investigations support the proposition that, in type II pneumonocytes, CMP is involved in integration of the synthesis of phosphatidylcholine and phosphatidylglycerol for lung surfactant. In the present investigation, the amount of CMP in rat type II pneumonocytes was altered directly and resultant changes in the synthesis of phosphatidylglycerol were examined. Type II pneumonocytes were made permeable to CMP by treatment with Ca2+-free medium, and phosphatidylglycerol synthesis was then assessed by measurement of the incorporation of a radiolabelled precursor, [14C]glycerol 3-phosphate, that was not effectively utilized by cells that resisted permeabilization. Incorporation of [14C]glycerol 3-phosphate into phosphatidylglycerol (but not into other lipids) was stimulated greatly by CMP (half-maximal stimulation at approx. 0.1 mM). CMP stimulated the incorporation of [14C]glycerol 3-phosphate into both the phosphatidyl moiety and the head group of phosphatidylglycerol. Incorporation of [14C]palmitate into phosphatidylglycerol was also stimulated by CMP. myo-Inositol, at concentrations found in foetal-rat serum (0.2-2.0 mM), inhibited CMP-dependent incorporation of [14C]glycerol 3-phosphate into phosphatidylglycerol and promoted, instead, CMP-dependent incorporation into phosphatidylinositol. These data, when extrapolated to foetal type II pneumonocytes, are consistent with the view that the developmental increase in the synthesis of phosphatidylglycerol for surfactant by foetal lungs is promoted by the increase in intracellular CMP and the declining availability of myo-inositol that were found previously to be associated with this period of development.

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“…Thus, in RCC and T-ALL, MYC reversibly decreased PIs, but in LC and HCC MYC rather increased these phospholipids as measured by DESI-MSI (Figures 5A and S5C; for tissue H&E staining, Figure S4B; for fold change, Figure S5D). PIs have inositol as the phosphate group substituent, and they are competitively synthesized with PGs from the common CDP-DAG pool (Figure 6A) (Batenburg et al, 1985;Liu et al, 2014;Quirk et al, 1984). Significant PGS1 and PTPMT1 induction may preferentially promote CDP-DAG conversion to PGs, which could then shunt metabolites away from PI synthesis.…”

Section: Myc Alters Glycerophosphatidylinositol Metabolism In a Tissu...mentioning

confidence: 99%

The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth

et al. 2019

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Effect of myo-inositol on the glycerophospholipid composition of adult and fetal rat lung tissue

Cited by 11 publications

References 19 publications

Gestational Age-Dependent Changes in Plasma Inositol Levels and Surfactant Composition in the Fetal Rat

Gestational Age-Dependent Changes in Plasma Inositol Levels and Surfactant Composition in the Fetal Rat

Cytidine monophosphate-dependent synthesis of phosphatidylglycerol in permeabilized type II pneumonocytes

The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth

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