Effect of mycorrhization on the isoflavone content and the phytoestrogen activity of red clover

Khaosaad, Thanasan; Krenn, Liselotte; Medjakovic, Svjetlana; Ranner, Alexander; Lössl, Andreas Günter; Nell, Monika; Jungbauer, Alois; Vierheilig, Horst

doi:10.1016/j.jplph.2007.08.015

Cited by 45 publications

(33 citation statements)

References 29 publications

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“…The inoculation with G. albida increased the production of total foliar flavonoids in ironwood seedlings, as also observed in red clover (Khaosaad et al, 2008). In the treatment with G. albida, 1.27 mg of flavonoids g plant .…”

Section: Discussionsupporting

confidence: 66%

“…The biosynthesis of phenolic compounds in medicinal plants can be improved by biotic factors, especially the AMF (Ceccarelli et al, 2010;Toussaint et al, 2007;Khaosaad et al, 2008) but the concentration of foliar phenols and tannins in ironwood do not vary among the inoculation treatments. Thus, the determination of mycorrhizal efficiency should be carried out before recommending mycorrhizal technology to increase the production of phenolic compounds in each plant species.…”

Section: Discussionmentioning

confidence: 99%

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Biotechnical application of arbuscular mycorrhizal fungi used in the production of foliar biomolecules in ironwood seedlings [Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz var. ferrea]

Silva¹,

Silva²,

Maia³

2014

J. Med. Plants Res.

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Libidibia ferrea, popularly known as ironwood is a medicinal plant of the Caatinga, in semi-arid Brazil. It is used for traditional medicine because it has bioactive compounds. Arbuscular mycorrhizal fungi (AMF) have been shown to improve the production of biomolecules in some plants. The objective of this study was to select efficient AMF for optimizing the growth and production of bioactive compounds in L. ferrea seedlings. Mycorrhization, mainly with Gigaspora albida, was efficient in improving the production of L. ferrea seedlings. It resulted in a larger stem diameter, higher chlorophyll a leaf content, higher amount of total proteins and flavonoids compared to that of non-mycorrhized seedlings. The biotechnological system using G. albida is an alternative for the production of L. ferrea seedlings, with increased levels of foliar flavonoids.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Biotechnical application of arbuscular mycorrhizal fungi used in the production of foliar biomolecules in ironwood seedlings [Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz var. ferrea]

Silva¹,

Silva²,

Maia³

2014

J. Med. Plants Res.

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“…Although red clover blossoms have been used in traditional herbal medicine for centuries, it is the semipurified isoflavone leaf extracts for relief of menopause-related symptoms that interest researchers. This positive effect on menopausal disorders has been confirmed by several clinical trials (Khaosaad et al, 2008). Wu et al (2003) identified 31 isoflavones in the leaves and stems, 25 in the roots, and 26 in the flowers of red clover, with the highest concentrations of formononetin and biochanin in the leaves.…”

Section: Introductionmentioning

confidence: 71%

Comprehensive evaluation of phytoestrogen accumulation in plants and in vitro cultures of Medicago sativa L. ‘Elçi’ and natural tetraploid Trifolium pratense L.

Çölgeçen¹,

Çalışkan²,

Kartal³

et al. 2014

Turk J Biol

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IntroductionHigher plants synthesize a wide variety of phenolic compounds during their regular growth and development. These phenolic compounds are products of the phenylpropanoid biosynthetic pathway and generally accumulate at relatively high concentrations in cell vacuoles, and they are usually chemically associated with sugars (Coronado et al., 1995). Flavonoids are found throughout the plant kingdom, whereas isoflavones are more restricted and particularly prevalent in the subfamily Papilionoideae of Fabaceae.The Fabaceae taxonomic class includes a variety of plants with significant economic value, including soybean, alfalfa, clover, pea, and various beans. These leguminous plants accumulate a wide range of phenolic secondary compounds, including isoflavonoid conjugates (Kessmann et al., 1990b). An important group of legume natural products, isoflavones, have been better understood at the molecular genetic level (Dixon et al., 1995;Dixon 1999;Dixon et al., 2004). These compounds function as preformed or inducible antimicrobial, antiinsecticidal compounds, signaling molecules in symbiotic nodulation by Rhizobium bacteria, or as allelopathic agents. In vitro cultures, which are a key method for secondary metabolite production, have been established for some of the legumes. Cell suspension cultures of Glycine max contained higher amounts of total isoflavones than that of the callus cultures (Federici et al., 2003). Genistein, daidzein, formononetin, and biochanin A were observed in the cell suspension cultures of Medicago truncatula (barrel medic), which is the model legume in plant functional genomics (Suzuki et al., 2005;Farag et al., 2007). T. pratense, usually called red clover, has a variety of isoflavones, including biochanin A, genistein, daidzein, and formononetin, which were reported in high concentrations (Setchell et al., 2001;Khaosaad et al., 2008). Isoflavones may act as an estrogen agonist or antagonist, depending on the compound and content present in the organism (Dog, 2005). Thus, red clover has become popular as a food supplement for the amelioration of menopausal disorders (Dornstauder et al., 2001;Beck et al., 2003). Although red clover blossoms have been used in traditional herbal medicine for centuries, it is the semipurified isoflavone leaf extracts for relief of menopause-related symptoms that interest

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“…17,18 Biotic www.soci.org NMM Saviranta et al stresses such as mycorrhization have been shown to increase the levels of biochanin A in red clover roots. 33 In alfalfa roots, CuCl 2 treatment caused the accumulation of medicarpin and formononetin aglycones, which were mainly produced by hydrolysis of their conjugated forms. 34 Effects of ozone exposure on phenolic concentrations varied between individual compounds and growth stages (Fig.…”

Section: Resultsmentioning

confidence: 99%

Red clover (Trifolium pratense L.) isoflavones: root phenolic compounds affected by biotic and abiotic stress factors

et al. 2009

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Effect of mycorrhization on the isoflavone content and the phytoestrogen activity of red clover

Cited by 45 publications

References 29 publications

Biotechnical application of arbuscular mycorrhizal fungi used in the production of foliar biomolecules in ironwood seedlings [Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz var. ferrea]

Biotechnical application of arbuscular mycorrhizal fungi used in the production of foliar biomolecules in ironwood seedlings [Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz var. ferrea]

Comprehensive evaluation of phytoestrogen accumulation in plants and in vitro cultures of Medicago sativa L. ‘Elçi’ and natural tetraploid Trifolium pratense L.

Red clover (Trifolium pratense L.) isoflavones: root phenolic compounds affected by biotic and abiotic stress factors

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