Effect of mutations in the pea genes Sym33 and Sym40

Jacobi, Lidija M.; Zubkova, Lidia A; Barmicheva, E. M.; Tsyganov, V. E.; Borisov, A. Y.; Тихонович, И. А.

doi:10.1007/s00572-002-0189-2

Cited by 19 publications

(1 citation statement)

References 27 publications

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“…There are some pea (Pisum sativum L.) mutants that are not able to develop symbiosis with both AM fungi and N-fixing bacteria. There are stages in both symbioses, controlled by the same genes including hyphal passage into the plant roots by appressorium (Myc1), the development of arbuscule, and mycorrhizal fungal development rate (Jacobi et al 2003;Borisov et al 2004). …”

Section: Establishment Of Dual Symbiosismentioning

confidence: 99%

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

Miransari¹

2014

Use of Microbes for the Alleviation of Soil Stresses

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Section: Establishment Of Dual Symbiosismentioning

confidence: 99%

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

Miransari¹

2014

Use of Microbes for the Alleviation of Soil Stresses

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Identification ofMedicago truncatulaGenes Required for Rhizobial Invasion and Bacteroid Differentiation

Horváth

Domonkos

Ayaydin

et al. 2015

Biological Nitrogen Fixation

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Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR

Seddas-Dozolme¹,

Arnould²,

Tollot³

et al. 2010

Molecular and Cell Biology Methods for Fungi

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Expression profiling of fungal genes in the arbuscular mycorrhiza (AM) symbiosis has been based on studies of RNA extracted from fungal tissue or mycorrhizal roots, giving only a general picture of overall transcript levels in the targeted tissues. Information about the spatial distribution of transcripts within AM fungal structures during different developmental stages is essential to a better understanding of fungal activity in symbiotic interactions with host roots and to determine molecular events involved in establishment and functioning of the AM symbiosis. The obligate biotrophic nature of AM fungi is a challenge for developing new molecular methods to identify and localize their activity in situ. The direct fluorescent in situ (DIFIS) RT-PCR procedure described here represents a novel tool for spatial mapping of AM fungal gene expression simultaneously prior to root penetration, within fungal tissues in the host root and in the extraradical stage of fungal development.In order to enhance detection sensitivity of the in situ RT-PCR technique and enable localization of low abundance mRNA, we have adopted direct fluorescent labeling of primers for the amplification step to overcome the problem of low detection associated with digoxigenin or biotin-labeled primers and to avoid the multiplicity of steps associated with immunological detection. Signal detection has also been greatly improved by eliminating autofluorescence of AM fungal and root tissues using confocal microscopy.

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Effect of mutations in the pea genes Sym33 and Sym40

Cited by 19 publications

References 27 publications

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

Identification ofMedicago truncatulaGenes Required for Rhizobial Invasion and Bacteroid Differentiation

Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR

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