Effect of Musa spp. extract on eggs and larvae of gastrointestinal nematodes from infected sheep

Abstract

ResumoAs helmintoses figuram como um dos principais entraves para o desenvolvimento da caprinoovinocultura. Haemonchus contortus é a espécie que causa maior impacto nesta atividade pecuária. A resistência às drogas antiparasitárias e a procura por alimentos de origem animal livre de resíduos tem elevado a importância de tratamentos fitoterápicos. O objetivo desse trabalho foi desenvolver um extrato de Musa spp. e avaliar através de testes in vitro o efeito anti-helmíntico sobre ovos e larvas de nematódeos gastrintestinais de ovinos. Foram colhidas amostras de fezes de ovinos, naturalmente infectados, para a obtenção de ovos e larvas, seguida pela realização do teste da eclodibilidade de ovos e o teste de inibição da migração larval. Nos testes in vitro foram observados a inibição da eclodibilidade larval nas concentrações de 160 e 180mg mL -1 de extrato e a inibição da migração larval nas concentrações de 800 e 1000 mg mL -1 . Os resultados indicam que o uso da folha de bananeira tem efeito anti-helmíntico e estudos in vivo acerca da aplicabilidade dessa tecnologia a campo devem ser feitos a complementar e trazer maiores informações ao que já foi revelado no presente estudo. Palavras-chave: Fitoterapia, Musa spp., clínica de ruminantes, resistência parasitária AbstractHelminthes are listed as one of the main problems facing the development of goat and sheep production. Haemonchus contortus is the specie that causes greatest negative impact in ranching. Resistance to antiparasitic drugs and demand for residue-free animal-derived food products has elevated the importance of herbal treatments. The aim of this study was to develop an extract of Musa spp. and assess by in vitro testing, the anthelmintic effect on eggs and larvae in the gastrointestinal nematodes in sheep. Stool samples from sheep naturally infected were used to obtain eggs and larvae and was then followed by a test of hatchability and a larval migration inhibition test. In vitro tests on the inhibition of larval hatchability at concentrations of 160 and 180 mg mL -1 of larval extracts and inhibition of migration at concentrations of 800 and 1000 mg mL -1 were observed. The results indicate that the use of banana leaf has an anthelmintic effect and that in vivo studies on the applicability of this technology to the field should be made to further understanding and bring more information to what has already been revealed in this study.

Anthelmintic and antioxidant potential of banana bracts (Musa paradisiaca) extract in ruminants

“…Extracts of the leaves and stem of Musa spp. showed inhibition of ovine nematodes (NEUWIRT et al, 2015). However, these authors used banana leaves and higher doses than those used in this study, i.e 160-180 mg mL -1 to evaluate the hatching test and 800-1000 mg mL -1 to evaluate the larval migration inhibition test.…”

“…However, for ovines, it was not possible to observe the influence on larval hatchability and migration, at any of the concentrations evaluated in the present study. However, despite these results, continuation of the study for this species is justified, mainly by evaluating higher doses and performing complementary tests, as anthelmintic activity was verified in previous researches (MARIE-MAGDELEINE et al, 2014;NEUWIRT et al, 2015;NOGUEIRA et al, 2012). The bracts of the heart of Musa spp.…”

“…The antiparasitic properties of some tanniniferous plants have been recorded by different research groups (GONÇALVES et al, 2016;U et 7 U et 5 U et al, 2018), specifically including Musa spp. (GREGORY et al, 2017;MARIE-MAGDELEINE et al, 2014;NEUWIRT et al, 2015;NOGUEIRA et al, 2012).…”

In vitro Anthelmintic activity of Musa balbisiana Colla (Square banana) against Haemonchus contortus eggs

“…They found a mean inhibition of 48.5%. Neuwirt et al (2015) reported a 100% inhibition in GIN eggs, with alcoholic extracts of Musa spp., using a 180 mg/mL concentration, a considerably higher quantity than that used in this study.…”

Ação in vitro do extrato do botão floral da bananeira (Musa spp.) sobre nematódeos gastrintestinais de ovinos

“…Estudos realizados com o objetivo de controlar parasitos e reduzir os impactos da resistência, têm relacionado diversas plantas taniníferas com propriedades anti-helmínticas, dastacando-se a bananeira (Musa spp.) (Neuwirt et al, 2015;Nogueira et al, 2012). De acordo com Niezen et al (1995) a utilização de plantas ricas em taninos condensados pode ser indicada como alternativa no controle de verminose em ovinos, reduzindo o uso de produtos sintéticos, bem como os custos de produção.…”