Effect of Musa acuminata Stem By Immunohistochemistry Test in Ulcer

Apriasari, Maharani Laillyza; Dachlan, Yoes Prijatma; Ernawati, Diah Savitri

doi:10.3923/ajb.2016.135.141

Cited by 9 publications

(14 citation statements)

References 5 publications

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“…This situation causes severe wound damage caused by too long hypoxia. 8,24 Macrophages can control signals in angiogenesis process during the formation of granulation tissue which occur from the 4 th until the 10 th day after a wound was formed. 21,25 Neovasculars during the process of formation of granulation tissue are controlled by many cytokines and growth factors.…”

Section: Discussionmentioning

confidence: 99%

“…5 Previous research indicates that gel extract of mauli banana stem (EMBS) can accelerate and increase epithelium thickness of the buccal mucosal wound healing in mice. [6][7][8] EMBS has bioactive materials of mauli banana consisted of 67.59% of tannin, 14.49% of saponin, 0.34% of alkaloids, 0.44% of ascorbic acid, 0.25% of flavonoids, and 0.006% of lycopene. 6 Condensed tannins can induce tyrosine phosphorylation of insulin receptor on the cell surface of 3T3-L1 adipocytes in vitro, so it can activate phosphatidylinositol 3-kinase pathway (PI3K), which plays a role in cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11] Previous research has also proven that the gel extract of mauli banana stem can increase the amount of HIF-1α, Hsp90α, and VEGF. 8 Based on these facts, the study aims to analyze the effects of the gel extract of mauli banana stem to increase the number of macrophages and neovasculars on mucosal healing in traumatic ulcers.…”

Section: Introductionmentioning

confidence: 99%

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The effect of musa acuminata stem in increasing macrophage and neovascular cells of healing process

et al. 2018

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Introduction: Musa acuminata stem has both of imunostimulatory and imunosuppressant effects that are influenced by their concentrations. The study aimed to analyze the effect of Mauli banana stem extract (EMBS) to increase the number of macrophage and neovascular cells in traumatic ulcer healing. Methods: Rattus norvegicus of male Wistar strain were used as model of traumatic ulcer. The left buccal mucous was biopsied, then used the histopathology method to find the number of macrophage and neovascular cells. Results: EMBS gel was effected when administered at a concentration of 37.5% because it significantly increased the number of macrophage and neovascular cells in traumatic ulcer healing on the 3rd day to 5th days. Conclusion: It can be concluded that EMBS within concentration of 37.5% can accelerate ulcer healing through the increasing number of macrophage and neovascular cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of musa acuminata stem in increasing macrophage and neovascular cells of healing process

et al. 2018

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“…Their left buccal mucosae were injured by means of a punch biopsy with a 6 mm diameter and 1 mm depth after being placed under general anesthesia ether. 5 The wound was ensured to be confined to the epithelium, without damaging the underlying muscle. Each of the four groups was then treated with MASE gel of varying concentrations three times a day (every 6-8 h); 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%.…”

Section: In Vivo Studymentioning

confidence: 99%

“…2,3 Moreover, MASE gel of 37.5% concentration can induce epithelium and fibroblast proliferation, as well as vascular endothelial growth factor in mucosal wounds of Rattus norvegicus, thus accelerating the wound-healing process. 4,5 The most abundant bioactive compound in MASE is tannin, which contains polyphenols. 2,3 The polyphenol showed anti-inflammatory effects through the inhibitory mechanism of tumor necrosis factor α (TNF-α) and nuclear factor kappa β (NF-κB) signal.…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect of Musa acuminata Stem

et al. 2020

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Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

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