Effect of multiple activation stimuli on the generation of Th1-polarizing dendritic cells

Paustian, Christopher; Caspell, Richard; Johnson, Terrence D.; Cohen, Peter A.; Shu, Suyu; Xu, Shuwen; Czerniecki, Brian J.; Koski, Gary K.

doi:10.1016/j.humimm.2010.10.004

Cited by 20 publications

(13 citation statements)

References 22 publications

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“…This finding suggests that an increased number of severe infections may drive and/or potentiate a Th-1 polarized or inflammatory response in PBMCs from XLA patients. Our results resemble those reported by Paustian et al, who demonstrated that repetitive exposure to LPS induces a burst of IL-6 production by normal monocyte-derived dendritic cells [26].…”

Section: Discussionsupporting

confidence: 92%

Increased Pro-inflammatory Cytokine Production After Lipopolysaccharide Stimulation in Patients with X-linked Agammaglobulinemia

et al. 2012

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Section: Discussionsupporting

confidence: 92%

Increased Pro-inflammatory Cytokine Production After Lipopolysaccharide Stimulation in Patients with X-linked Agammaglobulinemia

et al. 2012

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“…5 The impact of microbial products through Toll-like receptor leads to DCs that produce interleukin-12 (IL-12)/IL-23 and prime T helper type 1 (Th1)/Th17 responses. 7,8 In contrast, in the absence of inflammatory signals, 'semi-mature' DCs produce IL-10, which primes a regulatory T-cell response. 9 However, mediators other than cytokines and pathogens have a great impact on the physiology of DCs.…”

Section: Introductionmentioning

confidence: 99%

Leukotriene C4 prevents the complete maturation of murine dendritic cells and modifies interleukin-12/interleukin-23 balance

Álvarez¹,

Amaral²,

Langellotti³

et al. 2011

Immunology

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Summary Leukotriene C4 is an important mediator in the development of inflammatory reactions and ischaemia. Previous studies have shown that leukotriene C4 is able to modulate the function of dendritic cells (DCs) and induce their chemotaxis from skin to lymph node. In this study, we decided to evaluate the modulation exerted by leukotriene C4 on DCs, depending on their status of activation. We showed for the first time that leukotriene C4 stimulates endocytosis both in immature and lipopolysaccharide (LPS) ‐activated DCs. Moreover, it suppressed the interleukin‐12p70 (IL‐12p70) release, but induces the secretion of IL‐23 by DCs activated with LPS and promotes the expansion of T helper type 17 (Th17) lymphocytes. Furthermore, blocking the release of IL‐23 reduced the percentages of CD4+ T cells producing IL‐17 in a mixed lymphocyte reaction. Ours results suggest that leukotriene C4 interferes with the complete maturation of inflammatory DCs in terms of phenotype and antigen uptake, while favouring the release of IL‐23, the main cytokine involved in the maintenance of the Th17 profile.

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“…56,57 IL-12p70 is vital both in promoting Th1 polarization and in improving the ability of T-cell immune responses and IL-10 is a potent suppressor of T-cell function. 58,59 Synergism effect between poly (I:C) and CpG ODN for the activation of a macrophage lineage, which resulted in the synergistic induction of IL-12. These studies confirmed further exploration of the combined application of poly (I:C) and CpG ODN as immune adjuvant cells.…”

Section: Discussionmentioning

confidence: 99%

Addition of CpG ODN and Poly (I:C) to a standard maturation cocktail generates monocyte-derived dendritic cells and induces a potent Th1 polarization with migratory capacity

Zhu

Wang

et al. 2015

Human Vaccines & Immunotherapeutics

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Monocyte-derived dendritic cells (DCs) are used as immunoadjuvant cells in cancer vaccines and have made great progress. However, an optimal DCs subset is vital for this treatment effect, the current 'gold standard' cytokine cocktail DCs have a shortcoming in their cytokines secretion, especially IL-12p70, mainly because of the existence of PGE2. Therefore, it is necessary to find an appropriate DCs-based immunotherapeutic protocol. In this study, we compared a novel 'improved' maturation cytokine cocktail with the current 'gold standard' maturation cytokine cocktail used for generating standard DCs. The 'improved' maturation cytokine cocktail DCs showed a higher levels surface markers expression (CD80, CD83, CD86 and HLA-DR), the chemokine receptors CXCR4 and CCR7 and chemokine CCL19, CCL21 and CXCL21, whereas CCR5 expression was reduced. Most importantly, in contrast to 'gold standard' DCs, which secrete little IL-12p70 and as a result induce mainly Th2 immunity, 'improved' cytokine cocktail DCs secreted higher levels IL-12p70 and also secreted similar concentration IL-10. To removal of PGE2 from the 'improved' DCs did increase the IL-12p70 production. In conclusion, we here present the 'improved' DCs, as an optimal maturation cocktail protocol, can induce high migratory potential, generate immunostimulatory DCs, produce higher levels IL-12p70 with superior capacity to induce Th1 immunity, when compared with the 'gold standard' DCs.

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Effect of multiple activation stimuli on the generation of Th1-polarizing dendritic cells

Cited by 20 publications

References 22 publications

Increased Pro-inflammatory Cytokine Production After Lipopolysaccharide Stimulation in Patients with X-linked Agammaglobulinemia

Increased Pro-inflammatory Cytokine Production After Lipopolysaccharide Stimulation in Patients with X-linked Agammaglobulinemia

Leukotriene C4 prevents the complete maturation of murine dendritic cells and modifies interleukin-12/interleukin-23 balance

Addition of CpG ODN and Poly (I:C) to a standard maturation cocktail generates monocyte-derived dendritic cells and induces a potent Th1 polarization with migratory capacity

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