Effect of Multi-wall Carbon Nanotubes(MWNTs) on Structural and Mechanical Properties of Poly(3-hydroxybutirate) Electrospun Scaffolds for Tissue Engineering Applications

Abstract

Abstract. The aim of this study is to evaluate the e ects of Multi-Walled Carbon NanoTubes (MWNTs) on the structural and mechanical properties of poly-3-hydroxybutyrate (P3HB) electrospun sca olds. To achieve optimal properties of the electrospinning machine, P3HB polymer solutions were prepared at di erent concentrations and spinned in di erent electrospinning parameters. After optimization, MWNTs in di erent weight percentages (0.5%, 0.75%, 1%, and 1.25%) were added to the polymer solutions and electrospinned. The e ects of MWNTs on the structure of bers were investigated using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR) techniques. The addition of MWNTs increased the average ber diameter from 210 (neat P3HB) to 700 nm at 1.25% MWNTs. In addition, SEM photomicrographs and the MATLAB software program showed an increase in porosity from 81% to 84% in the presence of MWNTs. Tensile strength of P3HB/MWNTs composites revealed 158% improvement over pure P3HB sca old. According to mechanical and structural properties, the best amount of MWNTs was 0.5 wt%. Therefore, MWNTs with low percentages can signi cantly improve the mechanical properties of pure P3HB sca old, so that they can become favorable mechanically for tissue engineering applications.

Evaluation of structural and mechanical properties of electrospun nano-micro hybrid of poly hydroxybutyrate-chitosan/silk scaffold for cartilage tissue engineering

“…at the same condition, the maximum force of electrospun P3HB scaffolds was measured 3.28 N, while the maximum elongation was 1.71 mm. [36] It is obvious that all these mentioned scaffolds and their mechanical properties are not suitable for cartilage tissue engineering, but the mechanical properties of hybrid scaffolds are significantly better than the electrospun scaffold.…”

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

“…Many studies have designed and constructed PHB scaffolds. [ 28 29 30 31 ] In one of the first studies,[ 32 ] the researchers constructed three-dimensional scaffold made of alloy PHB/poly-(hydroxybutyrate-co-hydroxy valerate) (PHBV) by the electrospinning method. The prepared fiber scaffolds had more hydrophilicity than films made of the same material, which were prepared for comparison.…”

Evaluation of mechanical properties and cell viability of poly (3-hydroxybutyrate)-chitosan/Al2O3nanocomposite scaffold for cartilage tissue engineering

“…[45] One of the best methods to improve the mechanical properties of polymers is to make composites of them using ceramics as reinforcement phases. [456] One-dimensional nanostructures (nanowires, nanowhiskers, and nanotubes) have shown to be the most effective in enhancing mechanical properties due to their interdiffusion and entanglement in host polymer. [7] One of the most merits of electrospinning methods is the capacity of aligning one-dimensional nanostructures in the fibers.…”

“…[89] In addition to improving the physical and mechanical properties, these nanostructures can also enhance the cell response. [456] Alumina, as the first bioceramics, can be a proper choice due to its low cost, good compatibility, and high modulus. Alumina nanostructures have shown a significant increase in mechanical properties of poly (ε-caprolactone) and polymethylmethacrylate (PMMA).…”