Effect of multi arm-PEG-NHS (polyethylene glycol <i>n</i>-hydroxysuccinimide) branching on cell adhesion to modified decellularized bovine and porcine pericardium

Say, Sreypich; Suzuki, Mika; Hashimoto, Yoshihide; Kimura, Tsuyoshi; Kishida, Akio

doi:10.1039/d3tb01661g

Cited by 2 publications

(5 citation statements)

References 57 publications

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“…Given the above, the occurrence of the cross-linking reaction was confirmed. A high feed ratio of PEGDE was also found to contribute to the formation of ether cross-linkages …”

Section: Results and Discussionmentioning

confidence: 98%

“…A high feed ratio of PEGDE was also found to contribute to the formation of ether cross-linkages. 53 From the thermogravimetric analysis (TGA), it was observed that HD and HD-based cross-linked hydrogels generally exhibited two stages of weight loss (Figure S2). The initial weight loss occurred at approximately 100 °C, which was attributed to the evaporation of water molecules that were initially bound to the HD polysaccharide.…”

Section: Preparation and Characterizations Of Hd And Hd-basedmentioning

confidence: 99%

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Hemoadhican-Based Bioabsorbable Hydrogel for Preventing Postoperative Adhesions

Lu,

Wang,

Kong

et al. 2024

ACS Appl. Mater. Interfaces

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Postoperative peritoneal adhesions are a prevalent clinical issue following abdominal and pelvic surgery, frequently resulting in heightened personal and societal health burdens. Traditional biomedical barriers offer limited benefits because of practical challenges for doctors and their incompatibility with laparoscopic surgery. Hydrogel materials, represented by hyaluronic acid gels, are receiving increasing attention. However, existing antiadhesive gels still have limited effectiveness or carry the risk of complications in clinical applications. Herein, we developed a novel hydrogel using polysaccharide hemoadhican (HD) as the base material and polyethylene glycol diglycidyl ether (PEGDE) as the cross-linking agent. The HD hydrogels exhibit appropriate mechanical properties, injectability, and excellent cytocompatibility. We demonstrate resistance to protein adsorption and L929 fibroblast cell adhesion to the HD hydrogel. The biodegradability and efficacy against peritoneal adhesion are further evaluated in C57BL/6 mice. Our results suggest a potential strategy for antipostoperative tissue adhesion barrier biomaterials.

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“…Given the above, the occurrence of the cross-linking reaction was confirmed. A high feed ratio of PEGDE was also found to contribute to the formation of ether cross-linkages …”

Section: Results and Discussionmentioning

confidence: 98%

Section: Preparation and Characterizations Of Hd And Hd-basedmentioning

confidence: 99%

Hemoadhican-Based Bioabsorbable Hydrogel for Preventing Postoperative Adhesions

Lu,

Wang,

Kong

et al. 2024

ACS Appl. Mater. Interfaces

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“…The dPPC exhibits robust mechanical properties, it is easy to handle, and its size can be adjusted as per the application [42]. However, the enhanced biocompatibility of this membrane promotes strong adhesion between the liver, membrane, and abdominal wall.…”

Section: Discussionmentioning

confidence: 99%

“…On day 2, 100 µl of the culture medium was collected and mixed with 50 µl of Nano-Glo® HiBiT Extracellular reagent, and the luminescence intensity was measured using a multimode microplate reader. THP-1 cell density was quantified using the WST-8 cell counting kit, while fluorescence images were obtained with calcein-AM staining [42].…”

Section: Inflammatory Response Analysismentioning

confidence: 99%

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Investigation of anti-adhesion ability of 8-arm PEGNHS-modified porcine pericardium

Say,

Suzuki,

Hashimoto

et al. 2024

Biomed. Mater.

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In post-adhesion surgery, there is a clinical need for anti-adhesion membranes specifically designed for the liver, given the limited efficacy of current commercial products. To address this demand, we present a membrane suitable for liver surgery applications, fabricated through the modification of decellularized porcine pericardium with 20 KDa 8-arm PEGNHS. We also developed an optimized modification procedure to produce a high-performance anti-adhesion barrier. The modified membrane significantly inhibited fibroblast cell adherence while maintaining minimal levels of inflammation. By optimizing the modification ratio, we successfully controlled post-adhesion formation. Notably, the 8-arm PEG-modified pericardium with a molar ratio of 5 exhibited the ability to effectively prevent post-adhesion formation on the liver compared to both the control and Seprafilm®, with a low adhesion score of 0.5 out of 3.0. Histological analysis further confirmed its potential for easy separation. Furthermore, the membrane demonstrated regenerative capabilities, as evidenced by the proliferation of mesothelial cells on its surface, endowing anti-adhesion properties between the abdominal wall and liver. These findings highlight the membrane’s potential as a reliable barrier for repeated liver resection procedures that require the removal of the membrane multiple times.

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Effect of multi arm-PEG-NHS (polyethylene glycol n-hydroxysuccinimide) branching on cell adhesion to modified decellularized bovine and porcine pericardium

Abstract: Implanting physical barrier materials to separate wounds from their surroundings is a promising strategy for preventing postoperative adhesions. Herein, we develop a material that switches from an anti-adhesive surface to...

Cited by 2 publications

References 57 publications

Hemoadhican-Based Bioabsorbable Hydrogel for Preventing Postoperative Adhesions

Hemoadhican-Based Bioabsorbable Hydrogel for Preventing Postoperative Adhesions

Investigation of anti-adhesion ability of 8-arm PEGNHS-modified porcine pericardium

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