Effect of mucin extraction method on some properties of metronidazole mucoadhesive loaded patches

Arhewoh, Matthew Ikhuoria; Eraga, S.O.; Builders, Philip F.; Ibobiri, Mayor A.

doi:10.4314/jpb.v12i1.1

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…The method of Adikwu and Nnamani 23 was employed for the extraction with some modifications as described. 24 The African land snails (A. maginata) were washed with clean water and the shells were removed by breaking the apex and a metal rod was inserted to remove the fleshy body by swirled the rod clock-wise in the shell which forced the its meat part out of the shell into a bowel. The mucus layer (slime mucus) was gently scraped off from the fleshy parts by gentle press using distilled water, pooled together in a clean closed bowel and precipitated with chilled acetone.…”

Section: Extraction Of Snail Mucin (Slime)mentioning

confidence: 99%

Synergistic Interaction of Natural Snail Mucin and Lincomycin for Immuno-chemotherapy against Streptopneumococcal Infection: Checkerboard Evaluations

Gugu

Onwusoba

Onyi

et al. 2020

IJPI

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Introduction: Pneumonia is an acute infection of lung majorly caused by Streptoneumococcal bacteria or other microbes which present cough with phlegm (mucus), fever and other clinical symptoms. This mucus produced are easily polymerize with other similar natural product like snail mucin when taken which serves as a natural antimicrobial immune booster for enhanced or synergistic response against causative agent in the presence of antibiotics. This work was carried out to determine the combined effect of snail mucin as animal sourced natural product antimicrobial agent with lincomycin against different strains of Streptococcus pneumoniae as alternative treatment option for this respiratory tract infection "pneumonia" which has been a global challenge especially in the tropical Africa. Methods: Separate solution each of the two agent was prepared in sterile normal saline to get a different concentration of the agents, each solution contained twice the MIC of the agent. The agents were then combined in different ratios for interaction effect by adopting the standard continuous variation method and further diluted using twofold serially dilutions and evaluated against the test organisms using both agar diffusion and dilution method respectively. Results: The result of FIC index was calculated showed greater synergy in the equal ratio (50:50) combination against all the strains of tested organisms. Also, better performance at the combinations ratios of 60:40 and 40:60 of mucin and lincomycin respectively emerged. Conclusion: This synergistic effect has shown an improved natural product immuno-antimicrobial chemotherapy for alternative treatment option of such acute infection in the absent orthodox medicine.

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Section: Extraction Of Snail Mucin (Slime)mentioning

confidence: 99%

Synergistic Interaction of Natural Snail Mucin and Lincomycin for Immuno-chemotherapy against Streptopneumococcal Infection: Checkerboard Evaluations

Gugu

Onwusoba

Onyi

et al. 2020

IJPI

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“…It offers excellent biocompatibility, least toxicity, and is easily biodegradable when used for mucoadhesive systems [ 15 ]. It possesses hydrating, lubricating, and protective potentials against most disease-causing microbes [ 17 , 18 ]. Moreover, it has an excellent retention time, and a nonimmunogenic and nontoxic nature that makes it an ideal polymeric material for developing drug delivery systems in the pharmaceutical sector [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Tofacitinib Loaded pH-Responsive Chitosan/Mucin Based Hydrogel Microparticles: In-Vitro Characterization and Toxicological Screening

Malatani

Bilal

Mahmood

et al. 2023

Gels

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Tofacitinib is an antirheumatic drug characterized by a short half-life and poor permeability, which necessitates the development of sustained release formulation with enhanced permeability potential. To achieve this goal, the free radical polymerization technique was employed to develop mucin/chitosan copolymer methacrylic acid (MU-CHI-Co-Poly (MAA))-based hydrogel microparticles. The developed hydrogel microparticles were characterized for EDX, FTIR, DSC, TGA, X-ray diffraction, SEM, drug loading; equilibrium swelling (%), in vitro drug release, sol–gel (%) studies, size and zeta potential, permeation, anti-arthritic activities, and acute oral toxicity studies. FTIR studies revealed the incorporation of the ingredients into the polymeric network, while EDX studies depicted the successful loading of tofacitinib into the network. The thermal analysis confirmed the heat stability of the system. SEM analysis displayed the porous structure of the hydrogels. Gel fraction showed an increasing tendency (74–98%) upon increasing the concentrations of the formulation ingredients. Formulations coated with Eudragit (2% w/w) and sodium lauryl sulfate (1% w/v) showed increased permeability. The formulations equilibrium swelling (%) increased (78–93%) at pH 7.4. Maximum drug loading and release (%) of (55.62–80.52%) and (78.02–90.56%), respectively, were noticed at pH 7.4, where the developed microparticles followed zero-order kinetics with case II transport. Anti-inflammatory studies revealed a significant dose-dependent decrease in paw edema in the rats. Oral toxicity studies confirmed the biocompatibility and non-toxicity of the formulated network. Thus, the developed pH-responsive hydrogel microparticles seem to have the potential to enhance permeability and control the delivery of tofacitinib for the management of rheumatoid arthritis.

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“…Many recent studies on adjuvant development for hepatitis B vaccines are now focusing on the use of biodegradable polymeric particles (BPP) for adjuvants and have reported promising results [1]. Snail mucin being a natural mucoadhesive polymer can be classified as a BPP owing to its biocompatibility, non-antigenic/non-toxic nature as well as biodegradability in the living system [8]. Many recent studies have discovered snail mucin to possess wound healing and age renewing properties [9].…”

Section: Introductionmentioning

confidence: 99%

Experimental and molecular predictions of the adjuvanticity of snail mucin on hepatitis B vaccine in albino mice

et al. 2021

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Although aluminum-containing adjuvants are widely used in human vaccination due to their excellent safety profile, they exhibit low effectiveness with many recombinant antigens. This study investigated the adjuvanticity of snail mucin with recombinant Hepatitis B Vaccine (rHBsAg). Twenty-five (25) female mice distributed unbiasedly into 5 groups were used in the study and were administered different rHBsAg/Mucin formulation at 7 days intervals. Blood samples were collected a day following the administration for analysis. The results of liver function and body weight analysis were indications that snail mucin had no adverse effect on the mice. The treatment group (administer mucin and rHBsAg) showed significantly (P<0.05) higher mean titres of anti-HBsAg antibodies when compared with the negative controls and the positive control administered with two doses of rHBsAg after the boost doses (day 28). Furthermore, a comparable immune response to positive control administered with three doses rHBaAG was recorded. In silico prediction, studies of the protein-protein interaction of a homology modelled snail mucus protein and HBsAg gave an indication of enhanced HBV antigen-antibody interaction. Therefore, this study has shown that snail mucin possesses some adjuvant properties and enhances immune response towards rHBsAg vaccine. However, there is a need for further molecular dynamics studies to understand its mechanism of action.

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Effect of mucin extraction method on some properties of metronidazole mucoadhesive loaded patches

Cited by 6 publications

References 9 publications

Synergistic Interaction of Natural Snail Mucin and Lincomycin for Immuno-chemotherapy against Streptopneumococcal Infection: Checkerboard Evaluations

Synergistic Interaction of Natural Snail Mucin and Lincomycin for Immuno-chemotherapy against Streptopneumococcal Infection: Checkerboard Evaluations

Development of Tofacitinib Loaded pH-Responsive Chitosan/Mucin Based Hydrogel Microparticles: In-Vitro Characterization and Toxicological Screening

Experimental and molecular predictions of the adjuvanticity of snail mucin on hepatitis B vaccine in albino mice

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