Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig

Abstract

Adiponectin, the most abundant adipokine released by adipose tissue, appears to play an important role in the regulation of vascular endothelial and cardiac function. To date, however, the physiological effects of human monomeric adiponectin on the coronary vasculature and myocardial systo-diastolic function, as well as on parasympathetic/sympathetic involvement and nitric oxide (NO) release, have not yet been investigated. Thus, we planned to determine the primary in vivo effects of human monomeric adiponectin on coronary blood flow and cardiac contractility/relaxation and the related role of autonomic nervous system, adiponectin receptors, and NO. In 30 anesthetized pigs, human monomeric adiponectin was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure, and the effects on coronary blood flow, left ventricular systo-diastolic function, myocardial oxygen metabolism, and NO release were examined. The mechanisms of the observed hemodynamic responses were also analyzed by repeating the highest dose of human monomeric adiponectin infusion after autonomic nervous system and NO blockade, and after specific adiponectin 1 receptor antagonist administration. Intracoronary human monomeric adiponectin caused dose-related increases of coronary blood flow and cardiac function. Those effects were accompanied by increased coronary NO release and coronary adiponectin levels. Moreover, the vascular effects of the peptide were prevented by blockade of b 2 -adrenoceptors and NO synthase, whereas all effects of human monomeric adiponectin were prevented by adiponectin 1 receptor inhibitor. In conclusion, human monomeric adiponectin primarily increased coronary blood flow and cardiac systo-diastolic function through the involvement of specific receptors, b 2 -adrenoceptors, and NO release.

Relationship Between Retinal Blood Flow and Serum Adiponectin Concentrations in Patients With Type 2 Diabetes Mellitus

“…This was supported by a previous observation that intracoronary infusion of monomeric adiponectin caused a dose-dependent decrease in coronary vascular resistance and further resulted in a dosedependent increase in coronary blood flow in pigs. 39 Therefore, we speculated that adiponectin can increase the RBF by increased velocity induced by vasodilation of resistance vessels and dilated diameter of the measured retinal artery per se in males with type 2 diabetes with no and minimal DR.…”

Adiponectin receptor-mediated signaling ameliorates cerebral cell damage and regulates the neurogenesis of neural stem cells at high glucose concentrations: an in vivo and in vitro study

“…Subsequently, cells were exposed to D -glucose The cells were also treated with the AdipoR1 blocking peptide (3 μ g/ml, GTX89569-PEP, GeneTex, Irvine, CA, USA) for 4 days of the experiment. 132 …”

Can Adiponectin Help us to Target Diastolic Dysfunction?

“…Exogenous adiponectin has been used in several animal models [92][93][94]. However, adiponectin is a complex molecule that undergoes significant posttranslational modifications, making direct administration of adiponectin a particularly challenging and inconvenient treatment.…”