Effect of monoclonal antibodies on in vitro function of T-cell subsets

Eskra, Linda; O’Reilly, Kathy L.; Splitter, Gary A.

doi:10.1016/0165-2427(91)90103-j

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Antibodies and immunofluorescent staining. Monoclonal antibodies 16-1E10 (anti-CD2), and biotin-labeled C5B6 (anti-CD11c) were produced in our laboratories (18,19). Additional monoclonal antibodies were SBU-T8 (anti-CD8; University of Melbourne, Parkville, Victoria, Australia), CC15 (specific for the WC1 molecule on bovine ␥␦ T cells; Chris Howard, Institute for Animal Health, Compton, England), IL-A12 (anti-CD4; C. Baldwin) 3 Activation of virus-specific cytotoxic cells.…”

Section: Methodsmentioning

confidence: 99%

CD4⁺Cytotoxic T-Lymphocyte Activity against Macrophages Pulsed with Bovine Herpesvirus 1 Polypeptides

Wang

Splitter

1998

J Virol

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Bovine herpesvirus 1 (BHV-1) induces immune suppression, but the mechanisms for suppression are not well identified. We examined the induction and activity of BHV-1-specific cytolytic CD4+ T lymphocytes (CTL) by stimulating peripheral blood mononuclear cells (PBMC) of cattle immunized with attenuated live BHV-1. Cytolytic effector cells were primarily CD4+ T lymphocytes and lysed autologous, but not allogeneic, macrophages infected with BHV-1 or pulsed with BHV-1 polypeptides. Apoptosis of BHV-1-expressing target cells was observed in CD4+ CTL assays by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) analysis. To determine if apoptosis was mediated by a perforin- or Fas-mediated pathway, EGTA, a known selective inhibitor of the perforin pathway, was used. EGTA did not inhibit CD4+-T-cell-mediated cytotoxic activity, but it did limit the NK cell cytotoxicity of virus infected cells. These findings support the concept that CD4+ CTL lyse macrophages pulsed with BHV-1 polypeptides through a Fas-mediated lytic pathway by inducing apoptosis in the target cells. The prominent cytotoxicity mediated by CD4+ CTL suggests a mechanism of selective removal of viral antigen-associated antigen-presenting cells.

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Section: Methodsmentioning

confidence: 99%