Effect of monensin on the crop microflora of broiler chickens

Abstract

Summary &mdash; Experiments were undertaken to evaluate the effect of monensin on both the microorganisms and their metabolite production in the crop of 3-week-old broiler chickens. The addition of monensin to the feed (100 mg/kg) significantly (P < 0.05) lowered the number of lactobacilli in the crop and decreased the concentration of lactate, acetate, succinate and ethanol in both in vivo and in vitro incubations of the crop contents. The decreased acid production increased the pH of the digesta and also the pH of the monensin-supplemented cultures (P < 0.01 The decrease in the number of lactobacilli was accompanied by a concomitant proliferation of coliform bacteria and enterococci. The lactobacilli li counts returned to the pretreatment level within 5 days of monensin feeding. The counts of coliforms and enterococci, however, remained elevated. The concentration of lactate in the crop was lower and the pH higher in the monensin-fed chickens than in the control chickens (P < 0.005). The microbial metabolite production was lower in all the cultures supplemented with monensin, both in the control incubations and in those inoculated by the crop contents of monensin-fed chickens (P< 0.01 The effect of monensin in vitro ceased after 10 days of monensin feeding to the inoculi donors. In the presence of monensin, the crop environment was more suitable to the proliferation of coliforms, which might be a factor in the increased incidence of nonspecific septicemia. chicken / crop microflora / monensin Résumé &mdash; L'influence de la monensine sur la microflore du jabot de poulets. Nous avons étu-dié l'influence de la monensine sur les microorganismes et la composition des métabolites microbiens dans le jabot de poulets agés de 3 semaines. La diminution du nombre de lactobacilles dans le jabot ainsi que celle de la concentration en lactate, acétate, succinate, alcool éthylique et propionate in vivo et durant les incubations in vitro du contenu de jabots par l'addition de la monensine ont été significatives (p < 0,05). L'addition de la monensine a entraîné -en raison de la diminution de la production des acides -une augmentation du pH du contenu

The role of the crop in poultry production

“…This is an important part of the bird's life as they also are most susceptible to colonisation by non-desirable pathogenic and zoonotic organisms at that time (Gast and Beard, 1989;Smith and Tucker, 1980). Factors affecting speed of lactobacilli colonisation include dietary introduction of competitive Lactobacillus strains, prebiotics and organic acids affecting their competitiveness, medication (Rada and Marounek, 1996), and the nutrient content of the feedstuffs themselves (Rubio et al, 1998). It is worth noting that lactobacilli are fastidious bacteria with equally complex nutrient requirements as the host itself; they need to have access to simple sugars, amino acids and vitamins for growth.…”

“…It is worth noting that lactobacilli are fastidious bacteria with equally complex nutrient requirements as the host itself; they need to have access to simple sugars, amino acids and vitamins for growth. Lactobacilli can be eliminated by anti-bacterial agents such as penicillin and monensin, and when this occurs, the number of coliforms increases (Apajalahti and Kettunen, 2006;Rada and Marounek, 1996). Of note, Escherichia coli are capable of binding to crop squamous cells.…”

Crystal structure and FT-IR study of aqualithium 1-naphthylmethyl ester of monensin A perchlorate

“…It forms lipid-soluble pseudo-macrocyclic complexes with monovalent metal cations able to transport these cations across natural and artificial lipid membranes [1][2][3][4][5][6][7]. Sodium salt of monensin is used commercially as a coccidiostat for poultry and growth promoting agent for cattle [8][9][10][11][12][13].…”

Structure of 1-naphthylmethyl ester of monensin A complexes with monovalent metal cations

“…Monensin plays an important role as an Na + /H + antiporter and regulates many cellular functions. It is used in veterinary medicine as a growth-promoting and a coccidiostatic agent [3][4][5][6][7]. However, some unwanted side effects related to its use, such as degeneration of myocardial cells of domestic animals, have been also reported [3,4].…”