Effect of molecular weight on the antioxidant property of low molecular weight alginate from Laminaria japonica

Zhao, Xue; Li, Bafang; Xue, Changhu; Sun, Leilei

doi:10.1007/s10811-011-9679-y

Cited by 100 publications

(37 citation statements)

References 22 publications

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“…While the IC 50 of the other fractions (GLP and DAP) cannot be determined within the test dosage range (1–4 mg/L), which indicates that DAP and GLP have lower effect than SCP and ULLP on the removal of hydroxyl radicals, this may be due to GLP and DAP molecular weights being higher than that of SCP and ULLP. This result further supports the hypothesis that low molecular weight may function as effective antioxidants, and this is in agreement with results of Zhao et al and Zhang et al who demonstrated that the antioxidant activities have a certain relationship with molecular weight [43,44]. …”

Section: Discussionsupporting

confidence: 92%

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Chen

et al. 2016

IJMS

106

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Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material) on the process for extracting polysaccharides was investigated, along with the optimization of the extraction using the response surface method (RSM) with a Box–Behnken design. The polysaccharide structure, monosaccharide composition, degree of sulfation, and molecular weight (MW) distribution were analyzed by infrared (IR) spectrometry, gas chromatography (GC), and high-performance gel permeation chromatography (HPGPC). IR spectrometry showed that Sarcodia ceylonensis polysaccharide (SCP), Ulva lactuca L. polysaccharide (ULLP), and Durvillaea antarctica polysaccharide (DAP) were all sulfated polysaccharides and, except Gracilaria lemaneiformis polysaccharide (GLP), all belong to β-pyranosidic polysaccharides. The average molecular weight (MW) of SCP, ULLP, GLP, and DAP was 466, 404, 591, and 482 kDa, respectively. The quantitative and comparative results with external standards indicated that the main monosaccharide in SCP and ULLP was mannose; and GLP and DAP were mainly composed of galactose and glucose, respectively. Then the in vitro antioxidant activity of all of the polysaccharides was evaluated using different assays—2,2–azino –bis (3-ethylbenzthiazoline-6- sulfonate) (ABTS), hydroxyl radical, nitrite scavenging capacity, and reducing power—and the relationship between their antioxidant activity and chemical characteristics were also examined. ULLP presented the highest ABTS radical scavenging activity; ULLP, SCP and DAP also showed a strong effect on the ABTS radical scavenging activity. SCP and ULLP exhibited excellent hydroxyl radical scavenging activities, about 83.33% ± 2.31% and 80.07% ± 2.17%, respectively, at 4 mg/mL. The reducing power of DAP was relatively more pronounced than that of the three other polysaccharides. However, the nitrite scavenging activities of the four seaweed polysaccharides were weaker than other antioxidant activity (ABTS), hydroxyl radical scavenging capacity, and reducing power. In addition, GLP exhibited lower activities than the other three samples in all of the tests for the antioxidant activity.

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Section: Discussionsupporting

confidence: 92%

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Chen

et al. 2016

IJMS

106

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“…Sato and others () reported that the thermal stability of carp myofibrillar proteins was improved by conjugation to alginate oligosaccharide, which suggests that this protein modification improved the functional properties of fish myofibrillar proteins. In addition, Zhao and others () reported that this oligosaccharide could prevent lipid oxidation and scavenge hydroxyl (OH) and superoxide (O 2 − ) radicals. Furthermore, oligosaccharides prepared from alginate could be combined easily with ionic cross‐linking agents such as Ca 2+ and Mg 2+ in muscle proteins (Lee and others ) to form a stable 3‐dimensional network with a high water content.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the Cryoprotective Effects of Trehalose, Alginate, and Its Oligosaccharides on Peeled Shrimp (Litopenaeus Vannamei) During Frozen Storage

Zhang

Deng

et al. 2015

Journal of Food Science

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The cryoprotective effects of trehalose, alginate, and its oligosaccharides on peeled shrimp (Litopenaeus vannamei) during frozen storage was investigated by monitoring thawing loss, color, texture, myofibrillar protein content, Ca2+ -ATPase activity, and performing microscopic structural analysis. Data revealed significant (p < 0.05) inhibitory effects on thawing loss and textural variables (springiness and chewiness) in trehalose-, alginate oligosaccharides-, and sodium pyrophosphate-treated shrimp compared with the control and alginate-treated batches. L* values revealed that these saccharides had a positive effect on color stability during frozen storage. In addition, the results of chemical analyses showed that trehalose and alginate oligosaccharide treatments effectively maintained an increased myofibrillar protein content and Ca2+ -ATPase activity in frozen shrimp. In addition, hematoxylin & eosin staining and SDS-PAGE confirmed that these cryoprotective saccharides slowed the degradation of muscle proteins and the damage to muscle tissue structures. Overall, the application of trehalose and alginate oligosaccharides to peeled frozen shrimp might maintain better quality and extend the commercialization of these refrigerated products.

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“…AOS used as a dietary supplement has also been reported to beneficially affect the growth of weaned pigs, most likely by improving intestinal morphology and barrier function. In the same application, AOS inhibited mitochondria-dependent apoptosis of enterocytes [52]. In research related to human ageing, guluronic acid restored the expression of the superoxide dismutase 2, glutathione peroxidase 1, catalase, glutathione S-transferase, iNOS, and myeloperoxidase genes to normal levels, indicating that it might reduce the age-related changes [169].…”

Section: Other Activitiesmentioning

confidence: 94%

“…AOS (enzymatic degradation) with a molecular weight of less than 1 kDa are better scavengers of superoxide, hydroxyl, and hypochlorous acid free radicals than alginate with a molecular weight of 1-10 kDa, ascorbic acid, and carnosine. In addition, the AOS with molecular weight of 1-6 kDa have higher antioxidant activity against superoxide and hypochlorous acid free radicals than AOS with molecular weight of 6-10 kDa, even if they have a similar M/G ratio [52]. Thus, low molecular weight AOS exert higher antioxidant activity.…”

Section: Antioxidant Activitymentioning

confidence: 95%

Advances in Research on the Bioactivity of Alginate Oligosaccharides

et al. 2020

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Alginate is a natural polysaccharide present in various marine brown seaweeds. Alginate oligosaccharide (AOS) is a degradation product of alginate, which has received increasing attention due to its low molecular weight and promising biological activity. The wide-ranging biological activity of AOS is closely related to the diversity of their structures. AOS with a specific structure and distinct applications can be obtained by different methods of alginate degradation. This review focuses on recent advances in the biological activity of alginate and its derivatives, including their anti-tumor, anti-oxidative, immunoregulatory, anti-inflammatory, neuroprotective, antibacterial, hypolipidemic, antihypertensive, and hypoglycemic properties, as well as the ability to suppress obesity and promote cell proliferation and regulate plant growth. We hope that this review will provide theoretical basis and inspiration for the high-value research developments and utilization of AOS-related products.2 of 26 the 4 C 1 conformation and are linked by β-1,4-glycosidic bond, while in pG, all G residues are in the 1 C 4 conformation and are linked by an α-1,4-glycosidic bond. These features are responsible for the differences in their higher-order structure. For example, pG exhibits an egg-box-like conformation and usually forms stiffer 2-fold screw helical chains when dissolved in water, while pM forms belt chains through intra-molecular hydrogen bonds. Due to these dissimilarities, pM and pG, as well as their derivatives, will exhibit different activities [12].As the most abundant marine biomass and low-cost material, alginate has been extensively used in the food and medical industries. The widespread utilization is also driven by its favorable chemical properties and versatile activities. However, the applications of alginate have been greatly limited due to its high molecular weight and low bioavailability. Therefore, the degradation of high molecular weight polysaccharides into low molecular weight poly-or oligosaccharides is considered of great significance for improving their bioavailability, increasing the body's absorption of drugs, and fully utilizing the efficacy of polysaccharides. Recently, the degradation products of alginate, i.e., alginate oligosaccharides (AOS), have attracted increasing attention due to their biological activities and excellent solubility in water [13]. AOS can be depolymerized by different degradation methods, including enzymatic degradation, acid hydrolysis, and oxidative degradation [14]. Alginate lyases have been isolated from a wide range of organisms, including algae, marine invertebrates, and marine and terrestrial microorganisms, which can degrade alginate into unsaturated oligosaccharides by β-elimination [15,16]. Moreover, due to differences in degradation patterns, G content (G/M ratio), molecular weight, and spatial conformation of degradation products, AOS possess a variety of biological activities. They have anti-tumor properties [17], counteract oxidation [8], regulate immune respo...

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Effect of molecular weight on the antioxidant property of low molecular weight alginate from Laminaria japonica

Cited by 100 publications

References 22 publications

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Comparison of the Cryoprotective Effects of Trehalose, Alginate, and Its Oligosaccharides on Peeled Shrimp (Litopenaeus Vannamei) During Frozen Storage

Advances in Research on the Bioactivity of Alginate Oligosaccharides

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