Effect of modified murashige and skoog medium on chrysanthemum tissue culture

Abstract

Propagation of chrysanthemum through tissue culture can produce superior plants in large and uniform quantities and sterile cultures (motherstock) can be obtained so that they can be used as material for further propagation. This research was carried out at Tissue Culture Laboratory, Jember State Polytechnic from April to November 2022. The research design used was a Factorial Completely Randomized Design (CRD). The first factor is the variety which consists of 3 cultivar (Maruta Agrihort, Pinka Pinki and Suciono). The second factor was in vitro culture media consisting of 5 levels (control, MS+BA media 2.0 mg/L and IAA 1.0 mg/L, MS+BA 1.0 mg/L and IAA 1.0 mg/L media, MS+ banana extract media, MS+ coconut water media). So there were 15 treatment combinations with 3 replications for a total of 45 experimental units. Parameters observed included plant height, the day the shoots appear, the day the roots appear, number of leaves and number of roots. The conclusion this research is addition of organic matter to MS media was able to increase the growth of explants compared to MS media with the addition of synthetic PGR and the combination of MS media + coconut water had an average optimal growth yield on all parameters.