Effect of mitomocin C in eosinophilic nasal polyposis, in vivo: concentration of IL5 and GM-CSF, RT-PCR

Assunção, Evaldo; Castro, Mirian Cabral Moreira de; Araújo, Ricardo Nascimento; Guimarães, Roberto Eustáquio Santos; Nunes, Flávio Barbosa

doi:10.1016/s1808-8694(15)30032-x

Cited by 8 publications

(9 citation statements)

References 27 publications

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“…In addition to its well-known role as a chemotherapeutic agent, mitomycin C has been recently used as a complementary treatment for ENP patients after surgery. 11 Previous studies have illustrated that exposure to mitomycin C up-regulated the mRNA expression of chemokines in human corneal fibroblasts cultures in vitro and that these effects continued after mitomycin C removal. 37 Understanding the molecular mechanisms of the effects of mitomycin C on nasal mucosa tissue would benefit adjustments in clinical application of this woundhealing modulator and require further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…5,6 The use of mitomycin C has been previously suggested to delay closure of antrostomies in sinus surgery 7,8 in patients at the conclusion of sinus surgery to decrease the incidence of postoperative adhesion formation 9,10 and as a complementary treatment for ENP patients after surgery. 11 The topical application of mitomycin C has been recommended for a variety of ophthalmic diseases to decrease scar tissue after surgery. 12 Under these pathological conditions, mitomycin C acts as an antineoplastic and antibiotic agent because of its ability to alkylate DNA, which then prevents replication, transcription, and protein synthesis.…”

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confidence: 99%

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Impact of Mitomycin C on the mRNA Expression Signatures of Immunological Biomarkers in Eosinophilic Nasal Polyposis

Rocha-Silva

Gomes

Zauli

et al. 2013

Am J Rhinol�Allergy

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Impact of Mitomycin C on the mRNA Expression Signatures of Immunological Biomarkers in Eosinophilic Nasal Polyposis

Rocha-Silva

Gomes

Zauli

et al. 2013

Am J Rhinol�Allergy

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“…Nasal polyposis is a disease that can be seen in almost all age groups. Its incidence increases with age, peaking in the fourth decade (6). The mean age of patients with nasal polyposis in our study was 43.3 years.…”

Section: Discussionmentioning

confidence: 54%

Evaluation of autoinflammatory disease genes in nasal polyposis*

Köseoğlu¹,

Özcan²,

İkincioğulları³

et al. 2015

Turk J Med Sci

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Background/aim: To investigate cold-induced autoinflammatory syndrome 1 (CIAS1) gene polymorphisms that cause autoinflammatory diseases in patients with nasal polyposis (NP). Materials and methods:The study included 30 patients diagnosed with NP and 30 healthy age-matched individuals as a control group. CIAS1 polymorphisms were assessed by DNA sequence analysis. Patients with nasal polyps and the control group were compared in terms of gene polymorphisms. Each of the 8 polymorphisms of the CIAS1 gene was analyzed separately in the patient group. Results:The most frequently observed polymorphisms in the patient group were c.732G > A in 83%, c.663C > T in 23%, and c.1308C > A in 23% of the patients. c.732G > A polymorphism was evaluated separately. Guanine was transformed to adenine at the 732nd nucleotide position of the CIAS1 gene in the cDNA of chromosome 1. Conclusion:The CIAS1 gene c.732G > A polymorphism was thought to be responsible for an increase in disease susceptibility. The frequency of the "A" allele is higher in the patient group compared to the control group. Autoinflammatory diseases seem like a candidate to be one of these factors. This is the first report to define the role of autoinflammatory diseases among these factors.

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“…One of these mechanisms of actions of mitomycin-C is likely to be the lowering of the interleukins (IL-5 and GM-CSF) with reduction of the expression of proapoptotic proteins 17,32 . So Mitomycin-C enhances the suspension of antiapoptotic mechanism, thus making it possible for the apoptotic index in eosinophilic nasal polyp's eosinophils to grow.…”

Section: Discussionmentioning

confidence: 99%

“…It was possible to show the action of in vivo and in vitro mitomycin-C in diminishing the levels of both interleukin-5 and also granulocyte-macrophage colony-stimulating factor (GM-CSF) in eosinophilic nasal polyps. These oligoproteins diminish the rate of apoptosis in eosinophilic nasal polyps and prolong the survival of eosinophils [16][17][18] . The aim of this study is to evaluate the actions of in vitro mitomycin-C in the eosinophilic nasal polyp's eosinophils through autopaired experimental study with evaluation of the apoptotic index.…”

Section: Introductionmentioning

confidence: 99%

Apoptose em pólipos nasais eosinofílicos submetidos in vitro ao tratamento com mitomicina C

Anjos¹,

Vasconcelos²,

Crosara³

et al. 2012

Braz. j. otorhinolaryngol.

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The etiopathogenesis of eosinophilic nasal polyps is yet to be explained. Eosinophils are key components in the inflammatory infiltrate and are related to the perpetuation of the inflammatory process in chronic rhinosinusitis with nasal polyps.Objective: This paper aims to evaluate the in vitro action of mitomycin upon the apoptotic index of nasal polyps. Materials and Methods:This is a self-paired prospective experimental study using biopsy fragments from 15 patients with eosinophilic nasal polyps. Biopsy fragments were divided into two groups. In the case group, the fragments were treated with 400 µg/ml of mitomycin for five minutes. The control group fragments were treated with culture medium. The pair of fragments contained in the two first compartments -control and case -were immediately sent to the histopathologist. The other pair of samples containing control and case fragments was incubated for 12 hours. The fragments were then taken to the histopathologist for testing. The apoptotic index was determined by the morphometry in hematoxylin and eosin staining and DNA fragmentation analysis (TUNEL reaction). Results:The comparison between the two groups showed a statistically significant difference (p < 0,001) in the apoptotic index of the 12-hour incubated cultures. Conclusion:Mitomycin acts in vitro upon the eosinophilic nasal polyps inducing the rise of the eosinophilic apoptotic index. Braz J Otorhinolaryngol. 2012;78(3):32-7. ORIGINAL ARTICLE BJORL

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Effect of mitomocin C in eosinophilic nasal polyposis, in vivo: concentration of IL5 and GM-CSF, RT-PCR

Cited by 8 publications

References 27 publications

Impact of Mitomycin C on the mRNA Expression Signatures of Immunological Biomarkers in Eosinophilic Nasal Polyposis

Impact of Mitomycin C on the mRNA Expression Signatures of Immunological Biomarkers in Eosinophilic Nasal Polyposis

Evaluation of autoinflammatory disease genes in nasal polyposis*

Apoptose em pólipos nasais eosinofílicos submetidos in vitro ao tratamento com mitomicina C

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