Effect of miR-493-5p on proliferation and differentiation of myoblast by targeting ANKRD17

Zhuang, Xiaona; Xie, Fang; Lin, Zekun; Luo, Junyi; Chen, Ting; Xi, Qianyun; Zhang, Yongliang; Sun, J.X.

doi:10.1007/s00441-023-03777-3

Cited by 4 publications

(6 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results indicate that bbu-miR-493-5p promotes buffalo myoblast differentiation. Interestingly, this promotion of differentiation is contrary to the results of miR-493-5p in porcine myoblasts, found in Zhuang et al's study [25]. Therefore, we infer that the function of miR-493-5p is not conserved in different species.…”

Section: Discussioncontrasting

confidence: 93%

“…The CCK8 and EdU assays showed that its proliferation efficiency was increased compared to the control group. This promotion of proliferation is similar to the results of miR-493-5p in a study of porcine myoblasts by Zhuang et al [25]. During buffalo myoblast differentiation, we found that the overexpression of bbu-miR-493-5p significantly up-regulated the expression of the differentiation marker genes MyoD1, MyoG, and MyHC.…”

Section: Discussionsupporting

confidence: 91%

“…In recent years, miR-493-5p has also been found to be associated with skeletal muscle growth and development. For example, miR-493-5p promotes porcine myoblast proliferation and inhibits differentiation by targeting ANKRD17 [25]. Based on this, we inferred that it may play an important role in buffalo skeletal muscle development.…”

Section: Discussionmentioning

confidence: 78%

See 2 more Smart Citations

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Zhang,

Zhong,

Yao

et al. 2024

Animals

View full text Add to dashboard Cite

In recent years, the meat and dairy value of buffaloes has become a major concern in buffalo breeding, and the improvement of buffalo beef quality is key to protecting buffalo germplasm resources and solving the problem of beef supply. MiRNAs play a significant role in regulating muscle development. However, the precise mechanism by which they regulate the development of buffalo skeletal muscles remains largely unexplored. In this study, we examined miRNA expression profiles in buffalo myoblasts during the proliferation and differentiation stages. A total of 177 differentially expressed miRNAs were identified, out of which 88 were up-regulated and 89 down-regulated. We focused on a novel miRNA, named bbu-miR-493-5p, that was significantly differentially expressed during the proliferation and differentiation of buffalo myoblasts and highly expressed in muscle tissues. The RNA-FISH results showed that bbu-miR-493-5p was primarily located in the cytoplasm to encourage buffalo myoblasts’ proliferation and differentiation. In conclusion, our study lays the groundwork for future research into the regulatory role of miRNAs in the growth of buffalo muscle.

show abstract

Section: Discussioncontrasting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Zhang,

Zhong,

Yao

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…Related studies reported that the MED28 gene was associated with live weight in sheep [48]; The hypertrophy and conversion of postnatal muscle fibers largely determine the yield and quality of meat, we found that MED28 gene was related to muscle development [49]; QDPR is an enzyme that regulates tetrahydrobiopterin (BH4), a cofactor for enzymes involved in neurotransmitter synthesis and blood pressure regulation, therefore, QDPR genes are also important genes in the regulation of growth [50].Previous studies have shown that the LDB2 gene located at GGA4 was important to chicken growth traits, and the 31-bp indel was significantly correlated with multiple growth and carcass traits in the F2 resource population and affected the expression of the LDB2 gene in muscle tissue. It was also identified as an important candidate gene for rapid growth in chickens and had the strongest association with late body weight in Jinghai yellow chicken hens [17,21,[51][52][53].…”

Section: Genome-wide Association Analysis Of Growth Traitssupporting

confidence: 54%

Identification of candidate genes for feed efficiency and growth traits in Wenchang chickens

Cai,

liu,

Wei

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Wenchang chickens are one of the most popular local chicken breeds in the Chinese chicken industry. However, the low feed conversion efficiency is the main shortcoming of this breed. Therefore, we urgently need to find a more precise breeding method to improve the feed efficiency of Wenchang chickens. In this study, we explored important candidate genes and variants for feed efficiency and growth traits by genome-wide association study (GWAS) analysis. Results Estimates of genomic heritability for growth and feed efficiency traits, including residual feed intake (RFI), average daily food intake (ADFI), average daily weight gain (ADG), body weight at 87, 95, 104, 113 days of age (BW87, BW95, BW104 and BW113), ranged from 0.05 to 0.44. Important candidate genes were screened related to feed efficiency and growth traits were screened, including PLCE1, LAP3, MED28, QDPR, LDB2 and SEL1L3 genes. Conclusion The results identified important candidate genes for feed efficiency and growth traits in Wenchang chickens and provide a theoretical basis for development of new molecular breeding technology.

show abstract

“…In sheep, the MED28 gene was found to be associated with prenatal and postnatal body weights [ 47 , 48 ]. In pigs, the MED28 gene was found to be associated with muscle development [ 49 ]. There are no reports in the literature about the MED28 gene in chickens.…”

Section: Discussionmentioning

confidence: 99%

Identify Candidate Genes Associated with the Weight and Egg Quality Traits in Wenshui Green Shell-Laying Chickens by the Copy Number Variation-Based Genome-Wide Association Study

Yang,

Ning,

Yang

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

Copy number variation (CNV), as an essential source of genetic variation, can have an impact on gene expression, genetic diversity, disease susceptibility, and species evolution in animals. To better understand the weight and egg quality traits of chickens, this paper aimed to detect CNVs in Wenshui green shell-laying chickens and conduct a copy number variation regions (CNVRs)-based genome-wide association study (GWAS) to identify variants and candidate genes associated with their weight and egg quality traits to support related breeding efforts. In our paper, we identified 11,035 CNVRs in Wenshui green shell-laying chickens, which collectively spanned a length of 13.1 Mb, representing approximately 1.4% of its autosomal genome. Out of these CNVRs, there were 10,446 loss types, 491 gain types, and 98 mixed types. Notably, two CNVRs showed significant correlations with egg quality, while four CNVRs exhibited significant associations with body weight. These significant CNVRs are located on chromosome 4. Further analysis identified potential candidate genes that influence weight and egg quality traits, including FAM184B, MED28, LAP3, ATOH8, ST3GAL5, LDB2, and SORCS2. In this paper, the CNV map of the Wenshui green shell-laying chicken genome was constructed for the first time through population genotyping. Additionally, CNVRs can be employed as molecular markers to genetically improve chickens’ weight and egg quality traits.

show abstract

Effect of miR-493-5p on proliferation and differentiation of myoblast by targeting ANKRD17

Cited by 4 publications

References 68 publications

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Identification of candidate genes for feed efficiency and growth traits in Wenchang chickens

Identify Candidate Genes Associated with the Weight and Egg Quality Traits in Wenshui Green Shell-Laying Chickens by the Copy Number Variation-Based Genome-Wide Association Study

Contact Info

Product

Resources

About