Effect of miR‑449a‑mediated Notch signaling pathway on the proliferation, apoptosis and invasion of papillary thyroid carcinoma cells

Hou, Yunxia; Feng, Feiling; Yang, Ronghua

doi:10.3892/or.2019.7443

Cited by 3 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Xu et al [36] found that miR-449a inhibited the motility of breast cancer cells via regulating PLAGL2. As for PTC, Hou et al [18] demonstrated that miR-449a overexpression suppressed PTC progression via inhibiting Notch signaling pathway. We found that miR-449a was downregulated in PTC tissues and cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…As for PTC, Hou et al . [18] demonstrated that miR-449a overexpression suppressed PTC progression via inhibiting Notch signaling pathway. We found that miR-449a was downregulated in PTC tissues and cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…CircRNAs could serve as microRNA (miRNA) sponges to function in human malignancies [13,14]. Accumulating miRNAs were reported to be dysregulated in PTC, including miR-369-3p [15], miR-101 [16], miR-199a-5p [17] and miR-449a [18]. Hou et al [18] found that miR-449a accumulation restrained cellular malignant behaviors and induced apoptosis in PTC cells via inhibiting Notch signaling.…”

Section: Introductionmentioning

confidence: 99%

“…Accumulating miRNAs were reported to be dysregulated in PTC, including miR-369-3p [15], miR-101 [16], miR-199a-5p [17] and miR-449a [18]. Hou et al [18] found that miR-449a accumulation restrained cellular malignant behaviors and induced apoptosis in PTC cells via inhibiting Notch signaling. MiR-449a was a potential molecular target of circMAN1A2.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a

et al. 2022

View full text Add to dashboard Cite

Papillary thyroid carcinoma (PTC) is a common malignancy in endocrine system globally. Accumulating articles have found that circular RNAs (circRNAs) were dysregulated, and they were involved in PTC development. The aim of this project was to explore the function and associated mechanism of circRNA mannosidase alpha class 1A member 2 (circMAN1A2) in PTC progression. The expression of RNA was determined by real-time quantitative PCR. Cell proliferation ability was analyzed by colony formation assay and 5-ethynyl-2′-deoxyuridine assay. Cell migration and invasion were assessed by wound healing assay and transwell invasion assay, respectively. Protein levels were determined by Western blot assay. Dual-luciferase reporter assay and RNA immunoprecipitation assay were applied to confirm the interaction between microRNA-449a (miR-449a) and circMAN1A2 or metadherin (MTDH). Xenograft tumor model was utilized to explore the effect of circMAN1A2 silencing on tumor growth in vivo. CircMAN1A2 expression was elevated in PTC specimens and three PTC cell lines relative to adjacent normal specimens and Nthy-ori 3-1 cell line. CircMAN1A2 silencing inhibited the proliferation and motility of PTC cells. CircMAN1A2 acted as a molecular sponge of miR-449a, and circMAN1A2 knockdown suppressed PTC development partly through upregulating miR-449a. MiR-449a bound to the 3′ untranslated region of MTDH, and miR-449a restrained PTC progression partly through down-regulating MTDH. CircMAN1A2 interference suppressed PTC progression in vivo. CircMAN1A2 contributed to the proliferation ability and motility of PTC cells through enhancing MTDH expression via sponging miR-449a.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a

et al. 2022

View full text Add to dashboard Cite

show abstract

Role of the Notch ligands Jagged1 and Delta4 in Th17/Treg immune imbalance in a mouse model of chronic asthma

Zeng

et al. 2021

Experimental Lung Research

View full text Add to dashboard Cite

MiRNA-449 family is epigenetically repressed and sensitizes to doxorubicin through ACSL4 downregulation in triple-negative breast cancer

Eroles,

Torres-Ruiz,

Garrido-Cano

et al. 2024

Preprint

View full text Add to dashboard Cite

Despite progress in the breast cancer treatment, a significant portion of patients still relapse as a consequence of drug resistance. The involvement of microRNAs in cancer progression and chemotherapy response is well established. Therefore, this study aimed to elucidate the dysregulation of the microRNA-449 family (specifically, microRNA-449a, microRNA-449b-5p, and microRNA-449c-5p) and its impact on resistance to the commonly used chemotherapeutic drug doxorubicin in triple-negative breast cancer. We found that the microRNA-449 family is downregulated in triple-negative breast cancer and demonstrated its potential as a diagnostic biomarker. Besides, our findings indicate that the downregulation of the microRNA-449 family is mediated by the microRNAs-449/SIRT1-HDAC1 negative feedback loop. Moreover, it was found that the microRNA-449 family dysregulates the fatty acid metabolism by targeting ACSL4, which is a potential prognostic biomarker and mediated doxorubicin response through regulation of the drug extrusion pump ABCG2. Altogether, our results suggest that the microRNA-449 family might be a potential therapeutic target for the treatment of triple-negative breast cancer once it is implicated in doxorubicin response through ACSL4/ABCG2 axis regulation. Besides, our results also highlight the value of microRNAs-449 and ACSL4 as diagnostic and prognostic biomarkers in triple-negative breast cancer.

show abstract

Effect of miR‑449a‑mediated Notch signaling pathway on the proliferation, apoptosis and invasion of papillary thyroid carcinoma cells

Abstract: The present study aimed to investigate the effect of miR-449a

Cited by 3 publications

References 28 publications

circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a

circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a

Role of the Notch ligands Jagged1 and Delta4 in Th17/Treg immune imbalance in a mouse model of chronic asthma

MiRNA-449 family is epigenetically repressed and sensitizes to doxorubicin through ACSL4 downregulation in triple-negative breast cancer

Contact Info

Product

Resources

About