Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model

Nemezio, Mariana Alencar; Farias, Sofia Sampaio de Souza; Borsatto, Maria Cristina; Aires, Carolina Patrícia; Corona, Silmara Aparecida Milori

doi:10.1016/j.pdpdt.2017.10.025

Cited by 41 publications

(28 citation statements)

References 31 publications

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“…Incomplete bacterial reduction in biofilms has also been observed in many studies [ 6 , 7 , 36 , 37 , 40 , 41 , 43 ]. Our studies corroborate the literature since the main characteristic of cells organized in biofilms is the greater resistance to antimicrobials due to factors such as restricted penetration of antimicrobials, a decrease of the growth rate, expression of resistance genes and presence of persister cells [ 44 ].…”

Section: Discussionmentioning

confidence: 71%

“…One of the photosensitizers that is already heavily employed in studies involving aPDT in vitro [ 6 , 7 ] and in vivo [ 8 ] is methylene blue (MB). Its use has many advantages such as a high quantum yield, high water solubility, absorption of light at red wavelengths that allows great penetration of light in the tissues, becoming one of the most used photosensitizers in both in vitro and in vitro PDT studies [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

et al. 2018

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Chitosan (CH) is a biopolymer that exhibits a number of interesting properties such as anti-inflammatory and antibacterial activity and is also a promising platform for the incorporation of photosensitizing agents. This study aimed to evaluate the efficacy of antimicrobial activity of chitosan hydrogel formulation alone and in combination with the methylene blue (MB) associated with antimicrobial photodynamic therapy (aPDT) against planktonic and biofilm phase of Propionibacterium acnes. Suspensions were sensitized with 12.5, 25.0, 37.5, 50.0 μg/mL of MB for 10 min and biofilms to 75, 100 and 150 μg/mL for 30 min then exposed to red light (660 nm) at 90 J/cm2 and 150 J/cm2 respectively. After treatments, survival fractions were calculated by counting the number of colony-forming units. The lethal effect of aPDT associated with CH hydrogel in planktonic phase was achieved with 12.5 µg/mL MB and 1.9 log10 biofilm reduction using 75 µg/mL MB. Rheological studies showed that formulations exhibited pseudoplastic non-Newtonian behavior without thixotropy. Bioadhesion test evidenced that the formulations are highly adhesive to skin and the incorporation of MB did not influence the bioadhesive force of the formulations.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

et al. 2018

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“…As bactérias hidrolisam esse açúcar para produzir polissacarídeos extra e intracelulares. Neste contexto, os modelos de biofilme in vitro representam sistemas atrativos para investigar a eficácia de terapias antimicrobianas, visto que a viabilidade bacteriana e os fatores de virulência podem ser facilmente quantificados (Nemezio et al, 2017).…”

Section: Lesões De Cárie Em Dentina Produzidas Artificialmente In Vitrounclassified

“…Em relação ao Streptococcus mutans, a clorexidina se mostra capaz de interferir em sua atividade metabólica e reduzir efetivamente sua viabilidade, confirmando, assim, as suas propriedades antimicrobianas, uma vez que essa molécula é rapidamente atraída pela superfície bacteriana carregada negativamente, promovendo a ruptura da membrana e morte celular (Nemezio et al, 2017). A principal vantagem da clorexidina deve-se ao fato dela se ligar aos tecidos moles e duros da boca, permitindo que sua ação seja por um longo período após o uso de uma formulação que a contém na sua composição (Sharma et al, 2018).…”

Section: Clorexidinaunclassified

“…Embora seja usada como um padrão ouro em comparação a outros agentes antimicrobianos, a clorexidina possui algumas limitações quando do seu uso prolongado como enxaguatório. (Sharma et al, 2018;Cieplik et al, 2018;Nemezio et al, 2017;Fumes et al, 2018b). Porém, a clorexidina tem boa eficácia antibacteriana, efeito bacteriostático e efeito bactericida, devido à precipitação e / ou coagulação do citoplasma (Goud S, et al 2018).…”

Section: Clorexidinaunclassified

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Eficácia do uso de lasers ou clorexidina na redução microbiana em lesões de cárie produzidas artificialmente

Rodrigues¹

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Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

Xin

Kwek

et al. 2023

ChemPlusChem

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The emergence of multi‐drug resistant bacteria strains has been an uphill battle in modern healthcare worldwide, due to the increasing difficulty of killing them. The evolving pathogenicity of bacteria has led to researchers searching for more effective antimicrobial therapeutics to successfully eliminate them without undesirable consequences to the human body. In recent years, antimicrobial photodynamic therapy (APDT), an obsolete technique for cancer treatments, has been reported to eradicate bacteria and biofilm‐related infections. The principle of antimicrobial photodynamic therapy solely relies on the photosensitizers (PSs) generating reactive oxygen species, in the presence of oxygen and light, to destroy pathogens. Thus, it can target a broad spectrum of microorganisms, owing to the indirect interaction between PSs and the bacteria, resulting in the less likelihood for the development of drug resistant bacteria strains. This review will focus on the recent progress of APDT in the last five years and some future perspectives of APDT. The mechanism of APDT against bacteria and biofilms, various PSs used for APDT, and some common multidrug‐resistant bacteria strains will be briefly introduced. The reported in vivo applications of APDT in the several types of bacterial infections that includes periodontitis, wound infections, keratitis, endophthalmitis and tuberculosis in the last five years will be summarized in detail.

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Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model

Cited by 41 publications

References 31 publications

Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

Eficácia do uso de lasers ou clorexidina na redução microbiana em lesões de cárie produzidas artificialmente

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

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